We narrowed to 801 results for: DES

...Allewell and Sarma, 1974). Additionally, ThermoFisher provides a protocol on how to integrate RNAlater® with ...

...to the development and spread of cancer cells. Besides these stromal cell modifications, stromal cells...

...novel RNA-protein interactions, Ramanathan et al. designed an RNA bait containing three well conserved but...

...these difficult pieces: “It’s probably the best.” Besides working through the tough assignments, the skills...

... phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally PHO8 ...

...the postdoctoral population which keeps growing despite a lack of available academic positions.   Many...

...down your own results; are they the same as the description the authors give? Ask yourself if the results...

...Activation of membrane receptors and signaling cascades conceivably allows the genome to sense the impact...

... Parts. This library delivers 2 randomly paired guides per construct and allows a reduced scale in screens...

.../35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic...

... I can have on an organization beyond my job description.” Our resident bagel provider, source of energy...

...v) HCl Adjust to pH 2.0–2.2 For the mildest stripping solution, omit the Tween 20. Once your...

...that are built into how something functions, were designed for this purpose. One such control is dividing...

... and to work in a variety of applications. The desired specificity of your secondary antibody depends ...

...ELISA is a modification of the sandwich ELISA as described here, the direct and indirect ELISA (more details...

...uncomfortable places, from sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct...

...guanidine moiety at the end of its side chain. Decades ago, other labs discovered that the in vitro functions...

...for faster detection of RCA. 2nd Gen AdVs were designed to have increased cloning capacity, decreased  ...

...specific protein-protein interactions, SplitBioID provides a candidate list of proteins that are part of ...

...Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find...

...to a lay audience. My lay paragraph typically includes a couple of sentences of introduction to my area...

...materials required for each [1, 2]. This protocol [3] provides, in exquisite detail, the various steps required...

...communication and learning to work with people despite the distance improved my verbal and written communication...

...Derek Jacoby from Makerspace Victoria, CA Over the decades science has become increasingly restricted to academic...

...familiar with the minute details of your work. Describe the problem by stating the facts and explain how...

...Variant for Your Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional...

...specific details about the individuals, each example provides a scenario where both people get something valuable...

...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,...

... (about three to four days), this second round provides the added benefit of producing a stock P1 virus...

...Maria were encouraged by previous reports that described magnetic responses of cryptochrome proteins or...

...This will make it difficult to properly gate your desired cell population. By compensating between fluorescent...

...and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers...

...investigators to identify specific clones with desirable properties, either initially, or using secondary...

...(the Gal4/UAS system), and the third post will describe how you can make your own mutant flies. Find Drosophila...

...components into mammalian cell lines is quite broad and includes several different methods, so we've summarized...

...the site. Like Labguru, Quartzy offers software designed with scientists workflow in mind and can offer...

... to a new plate containing the mastermix which includes primers and a ddPCR supermix. Note that the supermix...

...minigenome system Minigenome systems, such as the one described here, are extremely useful research tools. In ...

...activation. Including a prey-only control also provides a baseline level of reporter activation. Vary ...

...that extra two miles which is way more fun. And despite those who may wish to tell you so – towel corners...

... plasmid delivery to a cell. In contrast, TREE provides a read out for plasmid delivery and subsequent...

...an abasic site. Like in most other base editor designs, the Cas9 nicks the non-edited strand to encourage...

...will identify a ‘scientific mentor’: someone who provides career advice and offers direction on your scientific...

...collaborators than you realize. Being at a university provides the perfect platform for practicing initiative...

... this re-inversion issue. It became possible to design FLEx vectors (Flip-excision vectors) that only ...

...with a site mutation (R11G). This site mutation destabilizes the ligase, allowing for active biotin molecules...

...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...

...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

...Biol 24, 758-766 e753 (2017). Baloban, M. et al. Designing brighter near-infrared fluorescent proteins: insights...

...if I had planned my day extensively, my lack of desire to go sit in the tissue culture hood may have prevented...