We narrowed to 610 results for: ACE;
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TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m....self-promoting—No one is going to tell you to pick up the pace Constant worry about grants and renewals What is... can be an issue in getting promoted (especially race and gender) Interpersonal issues can create interpersonal...
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Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....protein phosphatase that responds to rising intracellular calcium levels within the cell. When levels ... wild type (top panels) and cells with high intracellular Ca2+ (bottom panels) equipped with destabilized... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....Therapeutics, which was acquired by Ultragenyx Pharmaceutical in 2017. As one of the original employees at...impactful. At Novartis, Stegmeier deposited the ClonTracer Barcoding Library, a library that “enables the... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....contacting YNP staff to arrange permits to visit places not accessible to the public and retrofitting our...protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable between... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m....level of intimacy that creates the magic of a safe space. No matter why, now is a great time to find a few...Funding Opportunities Open Education Database: 100 Places to Find Funding For Your Research University of... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog PostPublishedJan. 25, 2018, 2:09 p.m....successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently...kinetics using a multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....possible. Instead of crowding the interface with every possible option, we place the most important controls... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....professorship. But recently a downward trend in funding and space in academic research labs has more students looking...industry, and government on social media (Twitter, Facebook, etc.) to learn about available jobs, fields, ... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog PostPublishedJune 1, 2021, 1:15 p.m....help researchers working on COVID-19. Its origins trace back to March 2020, when the first coronavirus lockdown...appealing, join us and help make science a better place. You will receive opportunities to collaborate with... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....movement. When the ribosome moves along an mRNA, it displaces other RNA-binding proteins to allow translation...translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the fluorescent signal:... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m.... our mindfulness group is sticking with their Headspace practice even while working remotely. Social distancing...reproducibility best practices in your research. For a place to start, check out Reproducibility for Everyone... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....Return of Race Science is a terrifying and equally good wake up call about the science of race." - ... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m....susceptibility of biofilms formed on various structured surfaces. Plasmids expressing fluorescent proteins will...biomarkers, and recently, cancer research related to space medicine. Now he aims on tracking pollution in rivers... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m.... hires initially based on those who notice and embrace the “odd and extreme openness” of the lab (i.e ...are engrossed in a task. Things seem to fall into place frequently and you sometimes feel confident in your... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m....amplified to add 5′ and 3′ homology regions to the adjacent fragment or vector. 5′ overhangs are generated...higher temperature at which Gibson assembly takes place may limit formation of secondary structures at the... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....can see from Figure 2 that determining where to place your gate based on the dot plot would be complicated...Conventional vs Spectral Flow Cytometry Beyond Surface Labeling in Flow Cytometry Resource on addgene.... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....also sometimes called unnatural amino acids) are placed in a protein site-specifically to alter its function...using GCE in your experiments, GCE4All is a great place to start. They host monthly webinars, workshops,... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....adjustments in the physical organization of your workspace and your workflow can have a huge impact. Test... experiments at odd hours, used more secluded workspaces, or politely asked to not be disturbed when they... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m....annual flagship workshop. Our 2018 workshop took place in Boston - you can revisit the activities of our...get in touch with ComSciCon, find us on Twitter, Facebook, Instragram, or send an email to [email protected]...