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Showing: 301 - 320 of 569 results

  2. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ... question to ask is how the efficiency of PITCh-based genomic insertion would compare to CRISPR sticky-end...Mathieu, et al. (2015). “A Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human...

  3. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...and fluorescence. Biosensors: A wide array of GFP-based fluorescent biosensors has been designed to detect...separates mixtures of cells into distinct populations based on fluorescent signal. Thus, FACS can be used to...

  6. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...less photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases...specific antibody binding sites and primary antibody-based detection, smFPs create much brighter signals for...

  7. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...different ideas. We are a huge fan of the Golden Gate based MoClo system. The vectors mentioned in this blog..."The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...money. They also usually have a good event-planning base and will know how to reserve space, time events,...I think many of the students learned something. Based on the initial guidelines I received from the teachers...

  9. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...allowing for in vivo biotinylation through a plasmid-based biotin ligase. Biotinylated dCas9, with bound DNA...genomic region is another example of the many tools based on targeting of a genomic locus through a specific...

  10. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...arises here. Notwithstanding access restrictions based on permissions, however, in some cases it would ...provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged...

  11. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...extrapolate the concentration of the unknown sample based on its measured response. An example of this is ..., the more accurate the curve. Though it varies based on the assay, the ideal %CV is less than 15%. You...

  12. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources... Shokri A, Chotteau V. Antibody capture process based on magnetic beads from very high cell density suspension...

  13. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ... Other fields, often highly technical or skills-based ones, may not use them very often. Similarly, entry-level...or sharing relocation plans. They can be skills-based: if you're switching fields, you can use your cover...

  14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...demand model with weekend and holiday effects. Based on these observations, we considered the following...enable next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four...

  15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...genome and bind target DNA sequences using RNA-DNA base pairing. Separately, TnsABC forms a transposase ...tools such as Cas9-recombineering, CRISPRa/i, and base editing. Leveraging a unique marriage between distinct...

  17. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...consistent development and improvement of CRISPR-based technologies over the past decade. CRISPR screens... fact supported by the wide array of lentiviral-based CRISPR libraries available at Addgene. The large...

  18. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity Purity of...Schematic of the barcode matching system and PCR-based confirmation of transfer plasmids used in Addgene...

  19. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...additionally allow for visual selection of transformants based on expression of RFP in the seed (Emami, Yee and...inflorescence (the flowering portion of the plant) near the base of the stem. This buys you a few days until new ...

  20. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...1990s, researchers developed viral vector systems based on retroviruses like Moloney murine leukemia virus...actively dividing cells. Another type of vector, based on adenovirus, could infect non-dividing cells, ...
Showing: 301 - 320 of 569 results