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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 534 results for: CAR

Showing: 301 - 320 of 534 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...this protein compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated...with this encapsulated enzyme produced 20% more D-glucaric acid, suggesting that MPyV system can improve ...

  2. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure 1: Left: recombined...and adopts an open conformation in the absence of cargo. bioRxiv 2024.12.20.629623; doi: https://doi.org...

  3. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...can be transfected with your transfer plasmid, carrying your gene of interest, and the two helper plasmids...undergoing titering, to remove residual plasmid DNA carried over from the production process. For more details...

  4. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...15%, 25%, 40%, and 60% iodixanol solutions are carefully layered and then the viral suspension generated...digested to remove any residual AAV plasmid that was carried over from the AAV production process. Either SYBR...

  6. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it is expensive...marker can be removed by Cre-mediated excision for scarless tag insertion.   In addition to the obvious advantages...

  7. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling... 25877186. 4. Prior, Ian A., Paul D. Lewis, and Carla Mattos. "A comprehensive survey of Ras mutations...

  8. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ... PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, and the locus-specifc gRNA... Contransfect the PITCh vector with the vector carrying Cas9 and the PITCh- and locus-specific gRNAs Step...

  9. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...expect to see for the unknowns in your experiment. Carefully set up a standard curve dilution series that takes...increase the accuracy of your experiment.  Finally, carefully analyze the data and use the trendline that's ...

  11. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...translated protein. As depicted in the accompanying cartoon, they have a multitude of uses including (but not...handbooks for protein purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts ...

  12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...invaluable reagents with the scientific community. Note: Cary Valley and Mary Gearing contributed to the update...PMC4660269. 7. Joung J, Ladha A, et al., ‘Point-of-care testing for COVID-19 using SHERLOCK diagnostics....

  13. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...antibiotics is certainly a topic of great concern in healthcare today, but it doesn’t have to be some looming...resistance from the CDC. Antibiotic resistance can be scary to think about, but we can all do our part to learn...

  16. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...problem-solving process?  You may want to start by carving out time to tackle it. It’s easy to get distracted...been building your creativity all along. In my own career, my team has used these techniques to optimize ...

  17. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...Overview An immunoprecipitation reaction is typically carried out in one of two ways. Both methods utilize the...approach appealing, but the disadvantages must be carefully considered before using. For example, the tag ...

  18. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ... in cold conditions and preferably on dry ice. Careful use of your glycerol stocks When your lab adopts...experiments. The term ‘insert’ refers to plasmids carrying each part, ‘vector’ refers to acceptor plasmids...

  19. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...line is maintained for ~20 passages before being discarded and replaced with a new vial of early passage ...produced in the line will be taken offline and discarded. Confirmation of the transfer plasmid Addgene ...

  20. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...chromosomal DNA. Chromosomal replication in bacteria is carefully regulated to ensure that it occurs at the appropriate...DNA replication in bacteria, so the process is carefully regulated by a mix of positive and negative regulators...
Showing: 301 - 320 of 534 results