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We narrowed to 535 results for: CAR

Showing: 301 - 320 of 535 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...this protein compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated...with this encapsulated enzyme produced 20% more D-glucaric acid, suggesting that MPyV system can improve ...

  2. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...15%, 25%, 40%, and 60% iodixanol solutions are carefully layered and then the viral suspension generated...digested to remove any residual AAV plasmid that was carried over from the AAV production process. Either SYBR...

  4. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... exonuclease, a polymerase, and a DNA ligase to carry out a pooled set of ligations in an hour or less...one to simultaneously complete an ordered set of scarless ligations following digestion with Type II restriction...

  5. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling... 25877186. 4. Prior, Ian A., Paul D. Lewis, and Carla Mattos. "A comprehensive survey of Ras mutations...

  6. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it is expensive...marker can be removed by Cre-mediated excision for scarless tag insertion.   In addition to the obvious advantages...

  7. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...Sharpie to circle 12-48 individual colonies by (carefully) holding plates up to the light, where they appear...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in...

  9. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ... PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, and the locus-specifc gRNA... Contransfect the PITCh vector with the vector carrying Cas9 and the PITCh- and locus-specific gRNAs Step...

  10. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...expect to see for the unknowns in your experiment. Carefully set up a standard curve dilution series that takes...increase the accuracy of your experiment.  Finally, carefully analyze the data and use the trendline that's ...

  12. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...translated protein. As depicted in the accompanying cartoon, they have a multitude of uses including (but not...handbooks for protein purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts ...

  13. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ... in cold conditions and preferably on dry ice. Careful use of your glycerol stocks When your lab adopts...experiments. The term ‘insert’ refers to plasmids carrying each part, ‘vector’ refers to acceptor plasmids...

  14. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...chromosomal DNA. Chromosomal replication in bacteria is carefully regulated to ensure that it occurs at the appropriate...DNA replication in bacteria, so the process is carefully regulated by a mix of positive and negative regulators...

  15. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...This post was originally written by Maria Soriano-Carot in September 2016 and was updated by Rachel Leeson... career...

  16. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...line is maintained for ~20 passages before being discarded and replaced with a new vial of early passage ...produced in the line will be taken offline and discarded. Confirmation of the transfer plasmid Addgene ...

  17. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...problem-solving process?  You may want to start by carving out time to tackle it. It’s easy to get distracted...been building your creativity all along. In my own career, my team has used these techniques to optimize ...

  18. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...Overview An immunoprecipitation reaction is typically carried out in one of two ways. Both methods utilize the...approach appealing, but the disadvantages must be carefully considered before using. For example, the tag ...
Showing: 301 - 320 of 535 results