We narrowed to 568 results for: CHI
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TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....decide what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere...weight of your library plasmids. As long as you achieve sufficient coverage from your transformation, any...
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Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...be distinguished using spectral flow since the machine uses the full spectral signature, not just a narrow... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....setting—especially if job applications require teaching experience or high numbers of publications, which...developmental biology and is applying this towards researching neurodegenerative diseases. Additional resources... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production and purification... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m....components of the transcription and translation machinery. These include ribosomes, RNA polymerase, tRNAs...Expression Systems Recombinant protein expression in Escherichia coli: advances and challenges Production of recombinant... -
PCR Applications for Copy Number Variation Assays
TypeBlog PostPublishedApril 26, 2022, 1:15 p.m....and departments will have qPCR machines available for use. ddPCR machines are not as commonly available... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....research that aligns with your interests and goals. Reaching Out to Potential PIs Even if you’re applying to...you determine if the program is the right fit. Reaching out to PIs also means doing plenty of mini-interviews... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....still virgins. Females begin mating ~6 hours after hatching into their adult phase. It is important to clear...before the progeny begin eclosion, the process of hatching from their pupal case (~9 days after setting up... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....is to make it detectable. Visualization can be achieved using fluorescent microscopy - even in living ...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m....behavior of cancer cells, attacking the cellular machinery necessary for division. This approach also kills... University of California, San Francisco, is approaching the Ras problem by exploiting the different mutant... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m.... of "The world’s biggest and best children’s museum", The Children's Museum of Indianapolis. I drove passed... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....Determine what type of outcome you are trying to achieve: Do you want to permanently knock out or knock-... All of these outcomes can be most effectively achieved with different CRISPR components. Select the appropriate... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....with Golden Gate MoClo. The barrier for those switching to Golden Gate is a conceptual barrier. Sometimes... of the university impacts the number of women reaching senior positions. There has been a lot of engagement... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....limited in the level of resolution that they can achieve. This means that regardless of how powerful of ...evenly in all directions, aka isotropically. But achieving isotropic expansion depends on how well the gel... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....opportunities for his students. But he also found that his teaching responsibilities left him with limited time for...the U.S. Army, and hockey coach all shaped his teaching style, with the former offering excellent training... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab. gRNAs can be in vitro transcribed from ssDNA...publicly available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....experience. Can combines cons of multiple medias. Switching from one media type to another is cognitively ... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m.... Chemistry at Connecticut College who has been teaching and communicating science for over two decades...as pertinent now as it was over 300 years ago. Teaching by example No examination of the state of science... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....RNA that bind to a cell’s DNA, telling the Cas machinery where to cut. Fig. 2: Heatmap indicating...when interpreting findings, particularly when researching ancestry groups. And, of course, it’s imperative... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m....fluorophore can emit a limited number of photons before bleaching. The amount of information one can extract from...cell biologist can feel like a college student searching couch cushions for spare change, desperate to ...