Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 572 results for: CHI

Showing: 301 - 320 of 572 results

  3. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...components of the transcription and translation machinery. These include ribosomes, RNA polymerase, tRNAs...Expression Systems Recombinant protein expression in Escherichia coli: advances and challenges Production of recombinant...

  5. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...relatively high efficiency. These alterations are achieved through DNA degradation, thanks to the unique .... Cas3 or Cas9? Large genomic deletions can be achieved using Cas9, but Cas3 may be a better option. To...

  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J., Blouin-Tavel, ... Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R., Conlon, T.,...

  7. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...decide what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere...weight of your library plasmids. As long as you achieve sufficient coverage from your transformation, any...

  8. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...research that aligns with your interests and goals.  Reaching Out to Potential PIs Even if you’re applying to...you determine if the program is the right fit. Reaching out to PIs also means doing plenty of mini-interviews...

  9. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...be distinguished using spectral flow since the machine uses the full spectral signature, not just a narrow...

  10. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...setting—especially if job applications require teaching experience or high numbers of publications, which...developmental biology and is applying this towards researching neurodegenerative diseases.   Additional resources...

  12. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...behavior of cancer cells, attacking the cellular machinery necessary for division. This approach also kills... University of California, San Francisco, is approaching the Ras problem by exploiting the different mutant...

  14. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...Determine what type of outcome you are trying to achieve: Do you want to permanently knock out or knock-... All of these outcomes can be most effectively achieved with different CRISPR components. Select the appropriate...

  16. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...with Golden Gate MoClo. The barrier for those switching to Golden Gate is a conceptual barrier. Sometimes... of the university impacts the number of women reaching senior positions. There has been a lot of engagement...

  17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab. gRNAs can be in vitro transcribed from ssDNA...publicly available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins...

  18. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... Chemistry at Connecticut College who has been teaching and communicating science for over two decades...as pertinent now as it was over 300 years ago. Teaching by example No examination of the state of science...

  19. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....experience.  Can combines cons of multiple medias. Switching from one media type to another is cognitively ...
Showing: 301 - 320 of 572 results