We narrowed to 360 results for: CRISPR

1. Learning Technical Skills at a Lab Central Workshop

   Type

   Blog Post

   Published

   Feb. 20, 2018, 2:30 p.m.

   ...Lab Management Software for Biology Learn about CRISPR Pooled Libraries Resources at Addgene.org Find...

2. Improved Plasmid Maps Powered by SnapGene

   Type

   Blog Post

   Published

   June 29, 2017, 1:06 p.m.

   ...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display...

3. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   Published

   May 25, 2017, 2:30 p.m.

   ...genes for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging...

4. New and Upcoming Viral Vectors - May 2020

   Type

   Blog Post

   Published

   May 28, 2020, 1:15 p.m.

   ...now available as a lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human...should be come available in the next few weeks. CRISPRi pooled library The Dolcetto pooled library is now...

5. Predicting Adverse Reactions to Monoclonal Antibody Drugs

   Type

   Blog Post

   Published

   Feb. 13, 2024, 2:15 p.m.

   ...Resources Antibodies 101: Introduction to Antibodies CRISPR in the Clinic   ...

6. K. phaffii: Rising to the Occasion

   Type

   Blog Post

   Published

   April 4, 2023, 1 p.m.

   ...Resources on Addgene.org  Yeast-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids...

7. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   Published

   Dec. 15, 2017, 2:07 p.m.

   ...interactions with Alzheimer's, I found others using CRISPR to generate new knockout mice, using adenoviral...

8. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ...determine if a variant is causal for a given disease. CRISPR has made genomic SDM relatively straightforward...

9. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ...Blog Read the Plasmid 101 blog posts Check out CRISPR Meets Synthetic Biology: A Conversation with MIT...

10. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even...

11. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...

12. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type

    Blog Post

    Published

    June 20, 2017, 2:30 p.m.

    ...Service Tips for 1st Time CRIPSR User (By a 1st Time CRISPR User) Tips for Titering Your Lentivirus Preps ...

13. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type

    Blog Post

    Published

    June 1, 2017, 2:30 p.m.

    ... In mammalian cells this would be possible with CRISPR/Cas-based genome editing. Selecting a bright fluorescent...

14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type

    Blog Post

    Published

    Sept. 15, 2020, 1:15 p.m.

    ... comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit...

15. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ...efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system...

16. All in a Twist: dsRNA

    Type

    Blog Post

    Published

    Feb. 2, 2023, 2:15 p.m.

    ...021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA Interference ...

17. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic systems ...

18. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...testing in the lab will be greatly facilitated by CRISPR reagents and other specific gene sequences available...

19. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency...

20. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    Published

    May 30, 2023, 1:15 p.m.

    ...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics...