Skip to main content
Addgene
Showing: 301 - 320 of 511 results

  1. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...produce ultrasound contrast. That is, until earlier members of our lab - the Shapiro Lab - developed Acoustic...student studying bioengineering. They are both members of the Shapiro lab at Caltech.   References and...

  2. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...for Excellence in Molecular Plant Sciences since December 2020. His research focuses on the efficiency of...for Excellence in Molecular Plant Sciences in September 2019 as a Ph.D. candidate. Her research focuses...

  3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...cleaving genomic DNA in mammalian cells and zebrafish embryos, and they can be used to modify genomic loci that...SpCas9 (Amrani et al., 2018). When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic...

  4. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...with plasmids encoding FPs tagged with the plasma membrane targetin sequence derived either from p63(1-29...non-specific labeling. For labeling the plasma membrane, they tested different target sequences for improved...

  5. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...Members of the bacterial world produce an assortment toxins to claim territory or kill competing microorganisms... (hok), a long-living toxin that damages cell membranes; Suppression of killing (sok), a short-lived RNA...

  6. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...https://youtu.be/EHHhiawIdWM Saving salmon, one embryo at a time – A video by LeeAnne Thayer, a Ph.D. ...University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science: http...

  7. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...harboring this mutation are deficient in outer membrane protease VII, which reduces proteolysis of the...pLemo (CamR) Expression of toxic, insoluble, or membrane proteins  T7 Express   IPTG-inducible expression...

  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...cells.   GFP is big GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter...Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells." Science 296.5569 ...

  9. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...recommendations.  As you optimize your protocol, always remember to include controls to show that your antibodies...sample).  When choosing a primary antibody, first, remember that antibodies recognize very specific 3-dimensional...

  10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College of Medicine. Several... guest blogger! Daria Shcherbakova is a faculty member at Albert Einstein College of Medicine. Her expertise...

  12. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...empathy, a willingness to listen, and an ability to remember the right stories at the right time. Like many...morefamousquotes.com A good listener absorbs and remembers and makes the teller feel heard. I am not always...

  13. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ... Symposium 2015. I personally participated as a member of the Career Paths Panel on the second day of ...people from all backgrounds are truly included as members of their research communities and are treated equitably...

  14. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...and equal protein transfer from the gel to the membrane. A loading control is critical for data interpretation.... Always probe the loading control on the same membrane that the target protein was measured on.    Flow...

  15. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...selection marker, then electroporate it into mouse embryonic stem cells. After selecting for clones with the...the mutation and inject the ES cells into a mouse embryo to create chimeric mice. This laborious process...

  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute.... While this seems obvious, it is important to remember that the same is true when designing gRNAs for...

  17. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...enhancers. Although the lab used this vector in chick embryos, it also functions in mammalian expression systems...vivo cis-regulatory interactions in the chicken embryo. BioRxiv: https://doi.org/10.1101/135525. Find...

  18. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...Show suitability for an intended application - Remember, just because your antibody performs as expected...is just as helpful as knowing when it will. And remember, antibodies are like puppies. Just because an ...
Showing: 301 - 320 of 511 results