We narrowed to 379 results for: ENA;
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TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....1997) with the goal of making the antibody more amenable to protein engineering. As a result, you can now...
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Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m.... other unique features. If your protein is not amenable to being tagged, e.g. tagging some proteins can... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....compared to a non-treated control group. In this scenario, gRNAs that are “enriched” correspond to genes... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....with techniques that make tissues transparent are enabling microscopes to visualize the cellular architecture... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....biology and genetics because the underlying advance enabling RNA-guided gene drives is Cas9 genome editing.... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....interest at high levels. Unlike vectors that express screenable or selectable markers from a unique promoter,... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....program uses a smaller word size and lower scoring penalties for mismatches and gaps in the alignment. If you... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....substitute for sound techniques, routine machine maintenance, and critical interpretation of the results. ... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m....of green fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly, cell biologists... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....beta-actin promoter of the pHLsec vector. In this scenario, IVA can be employed by cutting the vector with... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....making pretty pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....directed localization of the fluorophore, and thus enabled labeling of particular structures (Table 1). Each... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....et al. 2020); however, the technology potential enabled by CRISPR-transposons has yet to be fully realized... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....chemistry and evolution to illuminate biology and enable next-generation therapeutics. Prime editing, base... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....come to you (a TikTok scicomm account aimed at octogenarians is going to be a hard sell!) Digital platforms... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....Increasing the elongation time to 2 minutes and the denaturation time to 1 minute has been shown to increase ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....two halves of the luciferase protein interact and enable intein splicing (5). Screening with yeast two hybrid... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....-toxic and could be expressed in living cells, enabling the study of dynamic, physiological processes.... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....home no matter how busy I am. Believe it or not, teenagers just go to bed and you’ll never know it so don... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....plus additional automation in the backend, has enabled the QC to keep up with the pace of deposits while...