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  1. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...differ by only a few nucleotides, such as the fluorescent proteins EGFP and mEGFP. Many of these features...the gene, the name of its gene product, and a description of its function. You can also click one location...

  3. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ... articles reporting chromatin-related research describe the production of plasmids that might be useful...colleagues and guest bloggers have written posts describing new methods and tools for studying chromatin ...

  6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...lentiviral packaging methods. The three components described above (envelope, packaging, and transfer) are ...nomenclature of the plasmid backbone is meant to be descriptive of the components found within the backbone. ...

  8. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...immediately write a text document with a brief description of the directory contents, at a minimum. This...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...

  9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...diverges from previously discussed methods. As described above, you design two primers, each containing...vitro recombination. Across all modifications described in Trehan et al., REPLACR-mutagenesis’ median ...

  10. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ... for over 10 years. Many successful scientists describe having a “posse” of mentors as one key to their...Ellen Daniell. In “Every Other Thursday,” Daniell describes a group of 7 women scientists who met every other...

  11. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...mentoring questions I set out to answer. First, I will describe some of the many ways you can approach finding...Watch for my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle...

  13. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...talking about here? Many of the Fab fragments described above can be used for similar applications and...tissue-specific molecules identified by a double-label immunofluorescence technique for monoclonal antibodies.  J Histochem...

  14. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ..., non-dividing cells.   Figure 2: DNA rescue and transduction of a conventional single-stranded...Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually packaged...

  15. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA... and repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular...

  16. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...simple LED light. Lighting up your gRNA with fluorescent dyes In a scientific world where it often seems...seems like everything glows, why not spread the fluorescence to your gRNAs? Dye labeling the RNA in your ...

  17. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

  19. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...dividing and non-dividing cells. More detailed descriptions of the lentiviral elements and the 2nd and 3rd... deletion (Cre), together with expression of fluorescent or enzymatic reporters for cellular assays and...
Showing: 301 - 320 of 546 results