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We narrowed to 680 results for: Eng;

Showing: 301 - 320 of 680 results

  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...kDa). This system also requires less genetic engineering than some of the other systems reviewed here....PROTAC) was developed for HaloTag. PROTACs are engineered molecules which target E3 ligases to specific...that it requires an additional component to be engineered or expressed beyond the tag and small molecule...

  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated at the... however, are expensive and very technically challenging to use. Alyssa D. Cecchetelli is a Scientist... expression in Escherichia coli: advances and challenges Production of recombinant proteins in plant root...

  3. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into diverse bacteria...antibiotics are often essential genes which creates a challenge when studying them since their complete knockdown...about other CRISPR methods for bacterial genome engineering   Learn about human CRISPRko, CRISPRi, and CRISPRa...

  4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...Adenoviral vector DNA for accurate genome editing with engineered nucleases. Nat Methods 11:1051–1057 . https:/...Stanchina E, Lowe SW, Ventura A (2014) In vivo engineering of oncogenic chromosomal rearrangements with ...Tran K, Li J, Yin H, Anderson DG, Sontheimer EJ, Weng Z, Gao G, Xue W (2015) Adenovirus-Mediated Somatic...

  5. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... Steven Jacobsen and Jennifer Doudna’s labs engineered the tobacco rattle virus (TRV), a bipartite RNA...tissue tropism for gene therapy and ex vivo cell engineering applications and is especially well-suited for...perform neuronal tracing, anterograde tracing poses challenges, from non-selective anterograde transport to ...

  6. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...postings. Also, if you work in industry for any length of time, you’ll know more people in industry, which...in-depth discussion about your project might be challenging. In an academic environment, surrounded by lab...AstraZeneca, Pfizer, Novartis, Biogen, Amgen, New England Biolabs, Regeneron, and Genentech, among others...

  7. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...motivates you!), know their unique skills and strengths (you will likely have reports with different levels.... Personally I found this to be particularly challenging. As PhD students and postdocs we are used to ...was a manager! Second, my direct report was not engaged because I only left him/her with small tasks and...

  8. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...Collection. You can also use the library of full-length zebrafish rab proteins from Rob Parton's Lab (University...separated, most commonly FPs with red and green wavelengths, respectively, are selected (6). These previous...resolution of light microscopy is limited by the wavelength of light and, practically speaking, for common...

  9. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “Highly efficient...Non-homologous End Joining Read About Other Genome Engineering Tools like Sleeping Beauty Mutase Find the Ideal... genome engineering...

  10. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...mosquito vectors of diseases such as malaria and dengue are currently being performed in regions that don't...applications against scourges such as malaria and dengue for many years. Since more than a thousand children...selfish genes as tools for the control and genetic engineering of natural populations. Proc. Biol. Sci. 270,...

  11. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...alterations, bringing about a revolution in genome engineering, with new techniques rapidly being developed....and can be used for most of the current genome engineering applications of CRISPR including: generating ... Addgene.  Andrew's interests include genome engineering and microbiology.     References 1. Chen, Sean...

  12. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...find out! Homology-directed repair and genome engineering The easiest way to make a knock-in cell line ...the right and/or left of the knock-in site. The length of that sequence and the makeup (single vs. double.... A. Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....

  13. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...generating the standard curve for qPCR can be challenging to get right. Here at Addgene, we use a linearized...24328707. PubMed Central PMCID: PMC3991984. Song, Neng, et al. "Detection of circulating Mycobacterium ...droplet digital PCR for vaccine evaluation in challenged monkeys and TB diagnosis." Emerging microbes ...

  14. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...best plasmid for your research can be a daunting challenge. With over 80,000 deposited plasmids from around...That’s why we’ve built a new and improved search engine to help make this task a faster, easier, and frustration-free...sure you’ll find Addgene’s newly launched search engine results much better. Here, I wanted to find a specific...

  15. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...purify proteins can face any number of technical challenges depending on their protein of interest. After...After several decades of trying to address these challenges, researchers have amassed a considerable molecular...tripled increase the tag size quickly – modifying the length a polyhistidine tract does not greatly alter the...

  16. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...ICE on Synthego’s blog. CRISPR-based genome engineering has revolutionized the gene editing field by ...tool is part of Synthego’s full stack Genome Engineering Solutions, which also includes our CRISPR knockout...Browse our CRISPR featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility RNA Editing...

  18. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...share all of these new organisms continues to be challenging. Organism specific viral vector systems would...thus making progress on the NSF “G to P grand challenge.” Creating a productive, new scientific community...not just wait for papers to be published, but to engage in ongoing, real-time dissemination of information...

  19. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find the plasmids...indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color imaging with...

  20. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...genetic knockout  Knock-out cells or animals are engineered by introducing Cas9-mediated frameshift mutations...for PAM flexibility. Fig. 2: Prime editor engaging target DNA. The reverse transcriptase polymerizes...Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science (80-. ). 368, 290...
Showing: 301 - 320 of 680 results