We narrowed to 388 results for: HAL;

1. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...

2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

   Type

   Blog Post

   Published

   March 8, 2017, 3:15 p.m.

   ... (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic...

3. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   Published

   Aug. 30, 2016, 2:30 p.m.

   ...symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome. In response, the...

4. The Materials Science of Optogenetics Experiments

   Type

   Blog Post

   Published

   Sept. 17, 2015, 2:30 p.m.

   ...validation experiments we discussed last week are only half of the battle when using optogentics to answer a...

5. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   Published

   July 23, 2015, 2:30 p.m.

   ... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...

6. Hot Plasmids: Summer 2025

   Type

   Blog Post

   Published

   Aug. 28, 2025, 1:15 p.m.

   ...perform neuronal tracing, anterograde tracing poses challenges, from non-selective anterograde transport to ...

7. Hot Plasmids: Winter 2025

   Type

   Blog Post

   Published

   March 11, 2025, 1:15 p.m.

   ... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....

8. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   Published

   Feb. 4, 2025, 2:15 p.m.

   .... V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J...

9. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   Published

   Nov. 5, 2024, 8:32 p.m.

   ...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...

10. Antibodies 101: Flow Compensation

    Type

    Blog Post

    Published

    May 16, 2024, 1:15 p.m.

    ...Compensating between your fluorescent markers can be challenging! But we hope this guide can help you get started...

11. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    Published

    April 18, 2024, 1:15 p.m.

    ... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

12. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...for instance in inherited disorders such as B-thalassemia, Sickle Cells Disease, Duchenne Muscular Dystrophy...

13. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...

14. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...technologies currently available, you now face the challenge of trying to figure out what system might work...

15. PCR Applications for Copy Number Variation Assays

    Type

    Blog Post

    Published

    April 26, 2022, 1:15 p.m.

    ...available, ddPCR may be a better option for more challenging cases.  Price point If budget is a concern...

16. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...

17. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    .... Personally I found this to be particularly challenging. As PhD students and postdocs we are used to ...

18. Academic vs. Industry Postdocs

    Type

    Blog Post

    Published

    Nov. 13, 2018, 1:22 p.m.

    ...in-depth discussion about your project might be challenging. In an academic environment, surrounded by lab...

19. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ...in these species, they found that the N-terminal half of the antigen differs significantly between human...

20. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...different perturbations. Consider, for instance, the challenge of attempting to simultaneously and selectively...