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We narrowed to 777 results for: INA

Showing: 301 - 320 of 777 results

  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...nearly all cell types in the body from otherwise terminally differentiated cells simply by expressing four... a community largely created due to Yamanaka’s seminal work. It didn't take very long to realize that...Hideyuki Okano presented ground-breaking work on spinal cord injuries; Jessica Young shared research on..., a half-brother who is a quadriplegic due to a spinal cord injury, and numerous family friends who had...

  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...recent years, there are many fluorescent protein combinations that can be imaged together, but this also means...microscope(s) you intend to use. An accurate determination of whether two fluorescent proteins can be separated...Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / 561 / 640 nm). In ...try several proteins in your system of interest. Finally, in this set of proteins the green and red proteins...

  3. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...Inflammation and Immunity, as well as various combinations thereof (so called “Immuno-Oncology”) is exploding...tackle the challenge of curing cancer in cross-disciplinary projects and collaborations. Summarized by Professor...the boost it needs to help rid us of cancer in combination with chemotherapy. To dive deeper into this field...exciting development in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor...

  4. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...backbone with a T7 promoter driving multiple combinations of WT, codon optimized, untagged, cleavage sites... sites (thrombin and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include...found in the bacterial expression plasmids. Each combination is present in two sets of backbones which drive...expression system. Mammalian expression plasmids Finally a set of wild type and codon optimized, full length...

  5. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...antimicrobial.  Conjugates And HRP brings us handily to the final topic of this post, the conjugates, or signaling... a brief introduction to the wide, wild, and fascinating field of signaling molecules. Assay Type Westerns..., simple to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics: Antibodies...

  6. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ..., but it’s also found in tissues. IgG is the predominant isotype during a secondary immune response, which...switching is caused by a specialized type of DNA recombination. Heavy chain constant genes are clustered together... Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. B cells start by...heavy-chain constant domain gene cluster. DNA recombination can delete intervening heavy-chain constant ...

  7. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...lot in common. In fact, selections are often inappropriately categorized as screens! Why the confusion? ...Alternative screening techniques While CRISPR and drugs dominate the screening scene, there are other options which.... Non-functional traits will be automatically eliminated, unlike in screens. Selections begin and end ...require there to be a culture condition which eliminates manipulations that do not affect the function...

  8. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...sides of this promoter-gene combo are two long terminal repeats (LTRs). These LTRs define the limit of...respectively, act as RNA polymerase II promoter and terminator regions and are slightly different in sequence...expression constructs. Sandwiched between two inverted terminal repeats (ITRs) is the promoter and cargo, in that... it to evade detection by the host. Of note, recombinant adenovirus is missing the endogenous E1 gene,...

  9. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...continue to produce the specific antibody that the original B cell produced. Because of this, all of the antibodies...hybridomas producing the most specific antibodies. Finally, the selected hybridoma is expanded as a clonal...reproducible experimental results. When you are originally selecting a hybridoma, you can choose one that...from the hybridoma into a plasmid to create a recombinant antibody.     Figure 2: Changes to the...

  10. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...the page. Finally, as previously mentioned, enlist the help of your network to polish the final draft prior...don’t guess. Simply reach out to the program coordinator with your question(s). This precautionary step...notice within 24 hrs, send a note to the program coordinator.   6. Virtual interview prep Prepare for your...

  11. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...well as an efficient mechanism of homologous recombination to facilitate simple gene replacement/mutation...directly into the host chromosome via homologous recombination. Yeast Replicating plasmids (YRp):  These vectors...some antibiotics. Auxotrophy is defined as the inability of an organism to synthesize a particular organic...selection element pairs must be utilized or a new combination needs to be created in advance of the experiment...

  12. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...secondary will bind to all the primary antibodies indiscriminately, giving them the same output signal. In these... Time is a precious commodity in the lab and eliminating the need for secondary antibodies would save ...use a primary antibody labelled with a probe, eliminating the need for a secondary antibody. Other immunohistochemistry... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...

  13. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...convenient site for conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have the...If so, you are not alone. Full-sized antibodies dominate the world of research affinity reagents, and for...not a “Fab” fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide...

  14. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...post was originally written by Tyler Ford in March 2017 and updated by Angela Holmes and Christina Mork in..., spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High or Low Copy... with highly repetitive sequences (prone to recombination in bacteria) such as lentiviral, retroviral,...

  15. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...research focuses on understanding the structural determinants of protein-DNA interactions and how these interactions...functional portion of the Nfix gene using Cre recombinase and to generate Nfix-negative neural stem cells...developmental delay, result in loss of function or dominant negative NFIX phenotypes. Developing a rapid ...

  16. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...temperature, humidity, noise level, circadian effects (inactive versus active phase) Experimenter Interaction... of certain genes. A popular method uses Cre recombinase on transgenic flox animals to introduce modifications...- from basic types like locomotion and motor coordination to more complex situations such as learning ...Sena-Esteves M. Gene transfer to the CNS using recombinant adeno-associated virus. Curr Protoc Microbiol...

  17. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... something completely different than what was originally intended. Sharing unverified plasmids can lead... complete plasmid sequencing using an in-house Illumina MiSeq. The raw sequencing reads are assembled ...particularly difficult to sequence and assemble using the Illumina MiSeq platform, such as GC-rich regions and highly...short 200–250 base pair reads sequenced on the Illumina MiSeq.  Resolving QC issues If the QC team identifies...

  18. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ... proceeding to ultracentrifugation to avoid contaminating your transduction with the packaging cells. ...may be the result of plasmid integration via recombination during transfection, and does not necessarily...PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions...., Michael J. Yezzi, and Barbara R. Young. "Recombination events during integration of transfected DNA...

  19. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ... interest from its production site to its final destination. As an example, most secreted proteins are...pathway to their subcellular or extracellular destination. At the end of their life cycle, proteins may...through the vesicles to the lysosome where they are finally degraded (2, 3). Once you've created your fusion...

  20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...expressed, they predominantly fluoresce one color but slowly mature until they predominantly fluoresce a ...of protein fusions or expressed independently) originally fluoresce green or blue and, when they mature...hallmarks of ideal FP timers: 1. Ratiometric determination of expression time The ratio of mature to immature...
Showing: 301 - 320 of 777 results