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We narrowed to 811 results for: LARS

Showing: 301 - 320 of 811 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ... (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging...the epigenome may make certain processes, like cellular reprogramming of iPSCs, more efficient. In general...genes of previously uncharacterized enhancers. The popular TALEN-based LITE system, which uses light to regulate...genomic targets. These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9... use. If you want to study the effects of one particular modification for which a targeted editor, like...

  2. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...remains in the cytoplasm. Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor...Guzzo et al., 2020. Caulobacter crescentus is a popular model organism for studying the bacterial cell ...cell cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete ...CRISPR3 module of Streptococcus thermophilus (the popular Streptococcus pyogenes system does not work well...

  3. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...new way through the integration of science with a large set of other societal pressures including economic...p” policy is non-governmental work happening at large organizations who have to develop policies for their...policy to combat sexual harassment in academia or a large company who decides how they are going pursue regulatory...what better place to do that than at the nation’s largest integrated healthcare system? Defining your why...for skill development include leadership of extracurricular organizations, working in student government... impacts of health services research. She is particularly interested in driving scientific and technological...

  4. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ... the Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your STR profiles ...new resources with the scientific community – particularly ones that help address ongoing problems, like...catalogues Literature searches utilizing Google Scholar alerts and the NCBI LitSuggest Tool Following the...CLASTR results by minimal number of markers and similarity score match. This tool’s algorithm can be adjusted...

  5. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...Caveats of SunTag Very large size: a 24x scaffold fully occupied with GFP has a molecular weight of 1,400 kDa...to amplify fluorescent signals. Named for the "stellar explosion SUperNova," SunTag can help you turn ...time to get enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of...thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum et al. observed...

  6. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...bound to the intracellular surface of the GPCR. Binding of a ligand to the extracellular surface of the...environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...number of signaling cascades, inducing different cellular responses. One of the biggest advantages of such...Bioluminescence Resonance Energy Transfer (BRET). BRET is similar to Förster Resonance Energy Transfer (FRET), in...

  7. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...Vectors (or empty backbones) are frequently used in molecular biology to isolate, multiply, or express the insert...fusion protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3, comes...specific mutations. For these purposes Addgene has a large collection of yeast expression vectors with various...studying YGOI's function in worms. Some of the more popular studies involve using a reporter vector to tag ... Addgene: Plasmids 101 blog series Addgene's Molecular Cloning Guide & Other Plasmid Protocols List of...

  8. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...and are ready for a larger commitment, start your own blog. Plan to write regularly and, when you are ready...self-interested reasons, and, given that science is largely funded by tax-payers, there are reasons to feel...Twitter can be a good way to follow news about particular subjects or find out what others are reading....Natural Sciences and the Morehead Planetarium. Similar things are likely happening in your community, ...

  9. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...production can be a frustrating process that requires a large investment of time and resources for labs that don...Suggestion Form. Lentivirus As some of the most popular lentiviral vectors in the repository are used to... phenotype of interest. Optogenetic tools for similarly controlling neuronal activity with light will ... soon. We’re also distributing AAV preps with particular serotypes that can be used to deliver fluorescent...AAVs are getting to the right cells using your particular delivery technique and conditions. Finally, we...

  10. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...incomplete or nonexistent. In the 21st century, many molecular biologists didn’t know the complete sequences ... This algorithm enables one of SnapGene’s most popular aspects - its ability to annotate a raw plasmid...source of common features was our collection of popular plasmid sequences. These plasmids contain features...beginning or end of the feature. With the increasing popularity of gene synthesis, many researchers now use codon-optimized...Scientist at SnapGene, and also a Professor of Molecular Genetics and Cell Biology at the University of...

  11. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ..., SeeDB2 is not powerful enough for large brain samples. For large brain samples, we recommend pretreatment... to achieve highest expression levels. Clearing large tissues - Fluorescent proteins are very stable in...our SeeDB Resources website. We update the site regularly and hope Tetbow can help you in your research....assistant professor at Kyushu University. He has a particular interest in the development of sensory systems...

  12. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...cloned into a plasmid of interest. You can use similar processes to add overhangs to your insert of interest...primer design and the PCR process itself are very similar to those outlined in our restriction cloning post...based cloning     The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base...TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design is similar, but we need to use the reverse complement to get... get PCR amplification. We can start similarly, taking the final 18 bases of the ORF, including the stop...

  13. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... immune cells. Dendritic cells (DCs) are particularly popular whole cell therapeutics because they can...infections have resulted in the onset of cancer. Similar patterns of cancer development are observed in ...through these various signals. Over-production of vascular endothelial growth factors (VEGF) in tumors is...production, and CTLA4 and CD protein blocking. Other molecular targets under research include TIM-3, LAG-3, STAT...cell deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death...

  14. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...screening experiments, so the scale of the DNA prep is larger. And since the library contains many plasmids, ...amplification protocol, but the overall workflow is similar for most libraries. Here are the key points you... For libraries where the plasmids are all very similar sizes, like a gRNA library, this is less of a concern... hope you’ll agree with me that they look very similar to each other - that’s exactly what we want! At...your amplification. I hope this post has helped clarify some of the important considerations when using...

  15. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...colleague who preferred detailed instructions and regular check-ins, which helped them stay on track and ...resource: connecting with mentors who have made similar transitions can provide you with insights, lessons...mentoring a junior colleague. Guiding an intern, in particular, served as a great "baby project," offering a...strengths. By encouraging alignment on goals and clarifying roles, I eased the tension and improved collaboration... his PhD and a couple of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs...

  16. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...Type Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism...Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in Research More...

  17. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...libraries, large collections of biological independent variables, are becoming increasingly popular tools ...Viruses are the SUVs of molecular biology – they can deliver materials to specific destinations, haul...explored as the delivery mechanism for increasingly popular nucleic acid-based vaccines. These vectors provide...advantages over traditional vaccines, including 1) cellular responses in addition to antibody response 2) ...

  18. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...to studying membranes, organelles, and other subcellular compartments is via proteomics--the study of ... norovirus. While this assay is specific to a particular strain of norovirus, it can be tailored for the...segment The human proteome contains a breadth of modular domains that facilitate protein-protein interactions... and mediate biochemical processes. One of the largest and best characterized of these is the Src homology... sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought...

  19. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... Information Resources. ILAR Journal 46:258–268. https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari...for your experiment Monoclonal antibodies are particularly useful when you want to ensure that the antibody... the antibody, meaning that the antibodies are largely identical between lots.  Disadvantages of monoclonal..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are...

  20. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...is an app that helps you find internships and scholarships in different subject areas!    2. Resume...size, legibility, and more). Tips on drafting a stellar resume and cover letter can be found on the Addgene...through. The vast majority of programs tend to ask similar questions, thus devoting some time to writing a... for them, the likelier you will end up with a stellar letter of support.        5. Application submission...number of years as you apply for internships, scholarships, fellowships, and entry-level positions post...
Showing: 301 - 320 of 811 results