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We narrowed to 821 results for: LAS

Showing: 301 - 320 of 821 results

  1. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...both NeuroMab and the Allen Brain Atlas. The comparison with two atlases enables us to see the reproducibility...publicly available 2D/3D mouse atlas from antibodies that we screen. This atlas will enable scientists to ...and uploading verified antibodies onto the mAb3D atlas website. This website can then be used by thousands...distinct imaged pattern should align with what the atlases (mentioned above) and literature suggest, providing...distinct imaged pattern does NOT align with the atlases/literature and is unrelated to the target protein...of Giving As an open-source database, the mAb3D Atlas will foster collaboration, progress, and innovation...closer to resolving the antibody crisis. The mAb3D Atlas is a public service to the science community, and...

  2. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...Addgene.org Find plasmids from the Bethune Lab Find Plasmids from the Roux Lab Find Plasmids for FRET ...BirA* fusion proteins are expressed in cells via plasmids or by the creation of stable cell lines and these...interaction with the BirA* fused bait proteins ceases. Lastly, Split-BioID and BioID identify proteins that interact...Interested in using Split-BioID with your research? Plasmids are available from Addgene! References Schopp... Central PMCID: 5467174. Find the Split-BioID Plasmids Here! Munter, S.D., Görnemann, J., Derua, R.,...site than in Schopp et al. Find these Split-BioID Plasmids Here!   Burke, B., Kim, D.I., Roux, K.J., & Raida...PubMed Central PMCID: 3308701. Find the BioID Plasmids Here! Macneill, S., & Varnaitė, R. (2016). Meet...

  3. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...Fusions Plasmids 101: Secondary Nanobody Toolbox Resources on Addgene.org View the Antibody plasmid collection...The Seeger lab found a way around this by using plasmids to generate sybodies, synthetic nanobodies against...nanobodies can be synthetically expressed off of plasmids in bacteria, and they can target epitopes that...form of mRNA for academic research. The template plasmids for generating your own libraries are also available...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds...efficiently subcloned into the phagemid pDX_init plasmid, using the fragment exchange (FX) cloning technique... subcloned into pBXNPH3 or pBXNPHM3 expression plasmids for the production of tag-free proteins for further...

  4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...transposon system. This system has two parts: a plasmid expressing the Tn7 transposition genes (tnsA, tnsB... tnsB, tnsC, tnsD, and tnsE), and a plasmid that contains the Mobile CRISPRi cassette flanked by left ...copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient cells during a tri-parental...Mobile-CRISPRi ICE element forms a conjugative plasmid-like DNA circle and is transferred to recipient...transposon containing the CRISPRi components and a plasmid with the Tn7 transposition genes are transferred...RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the ability to synthesize diaminopimelic...

  5. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...Cascade-Cas3 plasmids in Addgene's collection! A "complex" system Cas3 is part of the Class 1 CRISPR family...complexity. Figure 1: Schematic of Class 1 CRISPR types. Class 1 includes type I with a Cas3 effector...out the brunt of the mechanical work for editing. Class 1 CRISPR systems make up the majority of CRISPR-...CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different types, with...making up type I, the most abundant type in the Class 1 category. These type Is can then be further broken... Various labs have designed all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann...Koonin, E. V. (2015). An updated evolutionary classification of CRISPR–Cas systems. Nature Reviews Microbiology...

  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...packaging plasmids: the pHelper plasmid containing Ad E2A, E4, and VA RNA genes; and the pRepCap plasmid containing...with your transfer plasmid, carrying your gene of interest, and the two helper plasmids, commonly called...blog Parts of the AAV Packaging PlasmidParts of the AAV Transfer PlasmidAAV Variables That Matter AAV protocols...called “triple plasmid transfection” (Figure 1).    Figure 1: Overview of key steps of AAV production, iodixanol...repeats (ITR). You should always have a high-quality plasmid standard and rAAV reference material of a known...before undergoing titering, to remove residual plasmid DNA carried over from the production process. For...

  7. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...labels! Reusing plastic Historically, scientists washed and reused materials such as glass pipettes or inoculation...that uses this waste as plastic lumber for things like truck bumpers, plastic adirondack chairs, and synthetic...other scientists save resources by sharing plasmids Find plasmids at Addgene ... popular theme in life sciences, some of these plastics are high grade and can be washed and reused. This...tips cost in half, not to mention the amount of plastic you’ve saved. Repurposing regulated medical waste...use this material to produce various grades of plastic lumber and distribute it to industrial and residential...

  8. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...is mediated by ADAR2DD, not PspCas13b. Find the plasmids for the RESCUE RNA editors here! Testing and improving...for viral delivery.  Find the RESCUE RNA editing plasmids here! Future directions Building on previous RNA...Science (2017):pii: eaaq0180. PMID: 29070703. Find plasmids from this paper at Addgene. Abudayyeh, Omar O...550(7675) (2017):280-284. PMID: 28976959. Find plasmids from this paper at Addgene. Abudayyeh, Omar O...27256883. PubMed Central PMCID: PMC5127784. Find plasmids from this paper at Addgene. East-Seletsky, Alexandra...) (2016):270-273. PubMed PMID: 27669025. Find plasmids from this paper at Addgene. Gootenberg, Jonathan...28408723. PubMed Central PMCID: PMC5526198. Find plasmids from this paper at Addgene. East-Seletsky, Alexandra...

  9. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...stability of modified Ti plasmids during plant infection, as well as plasmid yield during preparation ...GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids 101: Origin...Agrobacterium carries a “tumor-inducing” or Ti plasmid, which allows it to transfer genetic material into...in two species: Agrobacterium and E. coli. Like plasmids used in other systems, binary vectors are cloned...It’s a lot of information to fit into a single plasmid, so most first-generation binary vectors are quite...pSiM24 are suitable for many applications, the plasmid can also be customized due to its modular structure...Addgene Check out Addgene’s plant vectors! Find the plasmids from this paper at Addgene. Other relevant blog...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...protein in question: C2c1, C2c2 and C2c3 (short for Class 2, candidate 1, 2, or 3). C2c1 and C2c3 are related...to see this group of enzymes continue to make a splash in the CRISPR field. We feel that the culture of...27256883. PubMed Central PMCID: PMC5127784. Find plasmids from this paper at Addgene. 2. East-Seletsky,...) (2016):270-273. PubMed PMID: 27669025. Find plasmids from this paper at Addgene.  3. Gootenberg, Jonathan...28408723. PubMed Central PMCID: PMC5526198. Find plasmids from this paper at Addgene. 4. East-Seletsky,...) (2017):373-83. PubMed PMID: 28475872.  Find plasmids from this paper at Addgene.  5. Abudayyeh, Omar...550(7675) (2017):280-284. PMID: 28976959. Find plasmids from this paper at Addgene. 6. Shmakov, Sergey...

  11. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... unsure if I should possibly take a composition class in the English Department. He suggested that I sign...writing. Following his advice, I applied to the class. Receiving the email that I had been accepted into...Cotter, Mary O’Riordan, and Victor Di Rita, and, last, homework assignments. The instructors are leaders...microbiology with multiple high-profile publications. My classmates consisted of graduate students, post-grads, and...early career scientists. Over the course of the class, the moderator discussed individual sections of ...The assignments, which consisted of reading that class’s section of a manuscript and critiquing it, were...was time to work! Set up similarly to the online class, we met as a group for sessions covering individual...

  12. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ....12137. PubMed. Find the Plasmids at Addgene Kowarz Lab plasmids Izsvak Lab plasmids  ...the gene of interest (blue) in a plasmid backbone. A separate plasmid contains the transposase gene (red...). The original plasmid is empty following the removal of the transposon; the plasmid is then degraded...16 variants with different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent...for insertion in the correct orientation. These plasmids use a novel synthetic RPBSA promoter, which is...

  13. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    .... pBLADEONLY: This plasmid contains only BLADE and is based on the pTRC99a plasmid. It does not include...AraC and other promoter systems, head over to our Plasmids 101: Inducible Promoters article.) Modifying AraC...reporter downstream of the PBAD promoter. To use this plasmid with your gene of interest, you would replace mCherry...PBAD promoter. If you already have a pBAD33-based plasmid expressing your gene of interest, all you have ... and shared with Addgene. You can find all the plasmids deposited from Di Ventura's lab, including the...nuclear export system (LEXY) here. Optogenetic plasmids from the Khammash lab can also be found here. ...Learn more about inducible promoters Visit the Plasmids topic overview page Resources on Addgene.org ...

  14. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...the cytometer illuminates the passing cell with a laser and detects the light scattered by that cell at ...surface. Bear in mind, the voltage setting of the laser impacts the magnitude of FSC, SSC, and all fluorescent...experienced colleagues.   Figure 1: The laser pulse illuminates the cell and is scattered forward...the time that the cell spent passing through the laser beam. A is simply the area under the resulting curve... Figure 2: As the cell passes through the laser beam and causes it to scatter (FSC/SSC), the detected...), the time the cell needed to pass through the laser (W) and the area under the curve (A).   While... FSC/SSC plot, largely depends on cell type and laser voltage. The voltage describes an electric potential...

  15. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ... resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101 Biotinylation Other resources...insoluble proteins, which can be a challenge for classic antibody based affinity purification/mass spectrometry...vivo studies (Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and... specific locations in the cell.   Find BioID2 plasmids here APEX2: A more sensitive ascorbate peroxidase... They named this mutant APEX2.  Find the APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity...allowing for more precise temporal control.  Find plasmids for TurboID and miniturbo Split-BioID: A conditional...information, read the Split-BioID blog post. Find plasmids for Split-BioID RaPID: RNA-protein interaction...

  16. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...Addgene.org Find Fluorescent Protein Plasmids Check Out Other Hot Plasmid Technologies ...proteins and is driven by an inducible promoter (Plasmid 64542 or 64543) Before translation, both GFP and...contains a CAAX sequence to bind the mRNA to the plasma membrane; this sequence prevents diffusion of the...Since the SunTag-based system tethers mRNAs to the plasma membrane, it’s not appropriate for use with mRNAs... physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation...19213877 PMCID: PMC2746483 Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation...1367-71. PMID: 25792328 PMCID: PMC4451088 Find plasmids from this paper at Addgene. 3. Yan, Xiaowei, ...

  17. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...different plasmids. Harvard’s DRSC/TRiP Functional Genomics Resource Center has various plasmids for overexpression...gene of interest is cloned into a plasmid, you can send your plasmid to a company. For a few hundred dollars...Basically, you place your gene of interest in a plasmid and get the vector incorporated into the germline...filtering by fly as species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic... dollars, they will inject the plasmid into fly embryos and pick out flies where your transgene has been...incorporated into the germline. Outsourcing the plasmid injections is much easier than doing it yourself...Recombination Resources at Addgene.org Browse Drosophila Plasmids ...

  18. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ... ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV genome, ITRs serve as...stable 130 bp version is used in most plasmids. In an AAV vector plasmid, they flank the gene of interest....gene of interest in a cell or animal model. AAV plasmids contain several important elements, but among ...molecule as the gene that they package. In a transfer plasmid, the transgene is flanked by two ITRs, and the ...growth culture and result in a high percentage of plasmid DNA with mutated ITR sequences. Using recombinant-deficient...post for additional tips to reduce the risk of plasmid recombination. How do I validate my ITR sequences...located immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these...

  19. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...effector nuclease (TALEN) kit consisting of 834 plasmids. The researchers showed they could use their TALEN...particular DNA sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that allow you...the genome in rapid fashion. The two remaining plasmids in the kit have the rest of the protein in place...fluorescent proteins used to sort the cells after the plasmids have been introduced by transfection or electroporation...protein that binds those 15 base pairs is take four plasmids out of the library in the appropriate combination... ligation with the four inserts and the master plasmid in one step. The kit also has other unique aspects...you are designing a TALEN on paper to a finished plasmid. With our protocol, it is feasible to produce genetically...

  20. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...1). Plasmids: We generated a series of plasmids that are available from Addgene. These plasmids enable...Resources on Addgene.org Browse all CRISPR plasmids CRISPR plasmids for protein tagging Find validated gRNAs...Detailed protocols describing the use of these plasmids are available here. The Methods section of Nabet...cloning procedures used to construct the deposited plasmids. Molecules: We synthesized dTAG molecules by conjugating...terminus should be tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality...Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene tagging in human cells Resources...
Showing: 301 - 320 of 821 results