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... and suggest follow up experiments regularly? Can you work on some experiments of your own choosing? Does...scheduled or only ad hoc? Does the mentor keep appointments with group members or are they frequently cancelled...the department? Are they successful at getting grants and funding? Beware of bullying Your mental health...harassment or bullying. Even with the rise of movements such as the Future of Research Symposium and #...develop transferable skills by attending career events or being involved in a grad or postdoc association...there will be a way to grow and learn. Graduate students and postdocs in particular must care about this...

...different points in the pathway. Photoswitches are great for teasing out important branch points along a... to know about them to be successful in your experiments? In this post, we will discuss some broad classes...flexibility in using optogenetic tools in their experiments. Some tools turn on and off rapidly with light...determine which one(s) are candidates for your experiments.  Wavelengths Most vectors have an optimal wavelength... light stimulus. This can make or break your experiments!  First, closely read the publication, including...number of factors that can wreak havoc on your experiments, so it is important to understand the details...

... results with reference sequences To design experiments for further plasmid verification Identifying...process for incoming plasmids can vary based on the contents of a plasmid, the first step is always to send...for next generation sequencing. We then copy the contents of the resulting FASTA file into the desktop version.... Using Snapgene for additional verification experiments: Primer and restriction digest design Some regions...These regions require additional verification experiments that can be partially designed using the Snapgene...create custom annotations and design additional experiments like restriction digests. We’ll have a tutorial...

...methods and the types of experiments they are commonly used for.  Table 1. Points to Keep in Mind When Performing...Visualize components of individual cells Separation/identification of fluorescently-labeled components Fluorescence-activated...fluorescent protein for single and multi-color imaging experiments. Finally, what kind of system will you use to... Performing Biosensor Experiments Experiment  Detection Method Notes Quantification of a process ...

...weeks or every month is long enough that some experiments can get done and data analyzed between each meeting... from the lab members who are conducting the experiments to the PIs. Remote meetings can be tricky, and...communication tools such as Slack. Share data and documents While having multiple scientists edit the same... slower, sequential communication of emailing documents to one person at a time. Just be sure there is...preoccupied with other work or prioritize other experiments. Therefore, it’s crucial that everyone meets ... Some things to figure out are timelines for experiments and manuscripts, who is responsible for which...

...for this project, what would be your critical experiments and why? What techniques and skills do you ...another in a way not done before in your field?” He points out to “not be shackled by your own subject - maybe...applied to fungal biology.” Connie Cepko advises students to consider their goal of PhD training when selecting...” Professor George Church encourages graduate students and postdocs to follow their passions and dive...Research projects are designed to train graduate students and postdocs to think analytically and critically...elucidate which project is right for you.   Acknowledgments Thank you to Dr. Tom Ellis (Imperial College...

...useful for a wide variety of high-throughput experiments. There are currently 390 pooled libraries available...to identify specific cells — as well as their descendants. This makes them useful for lineage tracing, ...both mouse and human use. Each of these libraries accounts for chromatin, position, and sequence features...designed for all kinds of different high-throughput experiments. Our most popular screening library is the MORF...regulatory sequences ready to be assembled with other elements using golden gate cloning. This toolkit is designed...know which pooled libraries you’ve used in the comments! Resources Additional resources on the Addgene...

...as antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et...different parts together. By combining two optimized elements of an antibody probe, scientists can now more ...the widely used HA epitope in diverse cellular environments. They called this probe the HA frankenbody (... HA (smHA) which contains a 10xHA tag. These experiments demonstrated that the HA frankenbody can be used...tool-kit at Addgene to get started with your experiments! What else can you do with the HA frankenbody...

...highlights TTOs that accelerate science by processing agreements quickly! If your TTO receives a Golden Stopwatch...agreement. Below, you will find some easy talking points for broaching this subject with your institution...professionals: Describe to your TTO how unprocessed agreements slow down your research; provide specific examples...against one’s own record Mention Addgene Master Agreements. By default, your TTO must sign an agreement ...respective Master Reception and Master Deposit Agreements, your TTO signs each agreement just once, covering...the processing time of request MTAs and deposit agreements to basically instantaneous in many cases.  Suggest...

...extranuclear agents of heredity were thought of as everything from parasites, to symbionts, to genes and...Wollman and became the widely adopted name for these elements. At the time, the use of episome seemed appropriate...pSC102, with EcoRI and selected for E. coli transformants that were resistant to both. When this proved... vectors that could be used in these cloning experiments. Although plasmids started as a somewhat niche...that can be diversely applied to many different experiments. Addgene was founded in order to store, QC, curate...

...funding and space in academic research labs has more students looking for other options. While graduate programs...academic career development, it's often left to students to identify and build the necessary skills for...research from a broader viewpoint may expose which experiments are crucial in demonstrating the true innovation...entering the workforce increases, EAs allow graduate students and postdocs an opportunity to tailor their career...interests, and skills? Curious to know where graduate students and postdocs are currently getting hired? Let ...more about next. Leave your suggestions in the comments section.  ...

...viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should be used to clean...from workspaces. Speed: AAV transduction experiments are relatively quick. We have found that expression...benefits make AAVs useful for a wide variety of experiments, care still needs to be taken when selecting ...complexes are so robustly expressed that anatomical assessments can be easily performed too. While I implied ...transport of AAVs is a potential problem in many experiments, a group of researchers has identified this feature...used to treat disease in humans and additional improvements are being made to make them even more useful...Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional Resources on Addgene.org Find AAV...

...new NEB kit includes a step that both fragments the DNA and prevents it from forming concatemers later. ...services and expertise without breaking their bank accounts. When I heard about how LabCentral aims to enable...must first fragment a DNA sample into many small components. Without proper processing, these small DNA strands...produced high yields of quality libraries from small amounts of DNA i.e 100 pg. At the start of the workshop...itself. This was great because it forced workshop participants from different biotech companies to interact...

...smaller fragments that are then barcoded for identification. This mixture of barcoded fragments is known...help unlock the potential of modern sequencing instruments by enhancing the efficiency and simplicity of...known as a *library*. Parallel sequencing of the fragments within a library allows researchers to reconstruct...together to create a single library containing fragments carrying sample-specific barcodes. Rather than...is an author of 20+ scientific publications and patents.   ...

... none of the participants had preconceived opinions about “adeolitis.” The participants in my study were...used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight under the covers... affirm or challenge their beliefs. I asked participants to read short abstracts comparing the mortality...ideas about the controversial medical issues. Participants gave significantly more favourable ratings to...texts were otherwise identical. Likewise, when participants read an abstract for a study with findings that...

... important to know what kind of reagents you work with in experiments. Hence, I’m including this MSDS ...do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made by New England...AMINO can be beneficial for new researchers or students beginning to learn about biochemistry. The least...have a favorite lab app, please share it in the comments section below! Many thanks to our guest blogger...Pseudomonas aeruginosa. Follow him on twitter @100percentSDS.     Additional Resources on the Addgene Blog...

...collaboration - a sense of dialogue and community among participants, clarification - new and useful information,...them manageable. At Addgene, teams can add as many points as they like to the written agenda, but each only...and Accountability Agendas: Send around talking points for the meeting a day in advance. This gives everyone....  During the meeting, take notes on the agenda points. At Addgene, we like to do this live on the screen...for talking, not reading: Send around relevant documents, like proposals to review or project plans, before...send around a Google doc where people can make comments. At meetings, we just focus on the parts where...

...reported brightness measurements. Here, EGFP outperforms the improved folding variants, presumably due to.... A common requirement for live cell imaging experiments is the ability to follow multiple fluorescently... are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent...In mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best...channels used for more abundant proteins or marking compartments. I hope this sheds some light on multicolor ...

...substrates can permit measurements of luminescence from live cells. Luciferase variants with incompatible ...proteins offer different kinetics, substrate requirements, and photon emission wavelengths. In addition...analysis to investigate the effect of regulatory elements — such as promoters, enhancers, or untranslated...binding interaction brings the two luciferase components together. A similar approach can test protein-protein... substrates can also allow multiplexing with experiments that supply both substrates and measure luciferase...

...response elements (TRE) in a promoter. Tetracycline and its derivatives serve as inducing agents to allow...inactive because a bound repressor protein actively prevents transcription. Once an inducer binds the repressor...turned ON. Types of inducible promoters Chemical agents, temperature, and light are all examples of factors...HSF-1), which subsequently binds to heat shock elements in the promoter, thereby activating transcription...various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters and there is very...