We narrowed to 632 results for: Ncl

... infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and ...can also carry different types of CRISPR cargo including RNPs for indel formation via NHEJ, precise modification...

...order to see an effect. Hemizygous mice can be included in breeding strategies identical to those used...with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan, or infertility...

...sequences in DNA resulting in a variety of outcomes including excision or insertion, inversion, translocation...Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato...

... species of bacteria.  A note on anti-CRISPR nomenclature Acr family proteins are named for the type of...from pathogenic bacteria’s CRISPR-Cas system. Conclusion CRISPR is a great way to make those genome edits...

...plasmids! This category includes genes that help bacteria invade hosts, including attaching to host cells...

...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test... negative control to a sample with the primary included to confirm that the signal is from the primary...

...Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. These are ...are wells on the same ELISA plate that include known concentrations of your protein of interest. The optical...

...destroy. One treatment currently in clinical trials includes Cas3 (the shredder member of the Cas family) as... Other treatments currently in clinical trials include, but are not limited to, ones targeting muscular...

...of the Retroviridae or Retrovirus family, which includes the gamma-retrovirus and lentivirus genera, have... field of mouse transgenics. Experimental uses include: Cre-dependent gene expression, gene knockout, ...

...Arg-mutant proteins in living baker’s yeast cells, including Arg-mutant yeast homologs of human proteins that...frequently found in genetic testing for syndromes that include developmental delay, result in loss of function...

...Various soCoChR plasmids described in this work including the soCoChR-GFP plasmid as well as several derivatives...expresses N-demethylases and other required enzymes, including a glutathione S-transferase homolog from a Janthinobacterium...

...widespread use of FRET with fluorescent molecules, including fluorescent proteins, has led to the alternative...measuring the change in FRET efficiency, from which conclusions regarding the proximity of the two proteins can...

...plasmids use Rho-independent terminators, which include naturally occurring terminators, such as T7 and...mammalian terminators (SV40, hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes both polyadenylation...

...quantification tend to be overestimates since they can include free p24 and defective viral particles. Direct ...infectious virus in a prep as the measurement will include defective particles. Studies have found that values...

...biologists working together on a variety of projects including the OpenWorm project. The main goal of OpenWorm... track them. We have a number of microscopes, including an automated microscope but we need to develop...

...introduce yourself and what you do. You might want to include your university and your advisor’s name for some... uploading your work to a preprint server (and include this information on the poster) so your visitors...

...gRNAGFP. Gonçalves says that advantages of AdVs include their episomal nature and very efficient introduction...using the Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: ...

...website and also for offline use via GitHub (which includes prediction in batch mode). To predict the best...passion for computational biology. His expertise includes applying machine learning techniques to make sense...

...compensation between each other. We can therefore conclude that this population is double-positive for both...that there is no issue with compensation here. Conclusion Compensating between your fluorescent markers...

...possible, it uses standard tools and databases, including primer3, biopython, bwa, samtools, bowtie, UCSC...CrispyCrunch, be sure to check out the How To Guide. Conclusion CrispyCrunch is now publicly-available for high-throughput...