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We narrowed to 798 results for: OMP;

Showing: 301 - 320 of 798 results

  1. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... research compiled in the ELN. Without such references the record of research is incomplete, making it...commercialization office, the CIO’s office, and research computing. The two guiding principles emerging from the ...the record of research captured in the ELN is incomplete. Thus, to support reproducibility an ELN should...select and deposit individual documents or more complex datasets into the desired repository. They also...

  2. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...brainstorm session is to start with something completely unrelated to the issue and consider how it could...unique solutions to the same problem. If your lab/company/team is still working towards a culture of encouraging...just as valuable! In 2019, Addgene implemented a company-wide New Ideas Survey. Anyone could fill out the...of which, when implemented, impacted the entire company. It is important to note that the person who submitted... is an incredibly innovative group. Our entire company benefits from their policy! Perfectionism When ...

  3. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...sgRNA targeting the genomic site of interest. We compared the pCas/pTargetF system with the updated pEcCas...system.   Advantages of the pEcCas/pEcgRNA system Compared with the original system, the pEcCas/pEcgRNA is...strategy for plasmid curing, providing users with a complete editing process. The MUCICAT system While the ...dehydrogenase expression that is 2.6 times higher when compared to the industrial strain. In our metabolic engineering... pQCascade) with 4 rounds of transformation to complete the N-acetylglucosamine strain library construction...

  4. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...applying to the specific company or lab. Take some time to research the lab or company via their papers and...place to state why you’re applying to the job, company, and/or lab, and how you feel connected to their...apply for [title of the position] at [name of the company/organization]. I am a [adjective that describes... are interested in working for this lab and/or company. Remember to proofread your cover letter, and ...

  5. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...necessarily mean that you are a future employee of the company. It does, however, offer you an opportunity to ...like to work, get online and take a look at the company or institution’s career page, where you’re likely...openings. A good approach is to do this first for companies at which you’ve already made a personal connection...result in a recruiter giving you a call. Many companies enlist the assistance of recruiters for identifying...development center, if one exists. Get online! Review company and institution career pages. Make yourself visible...

  6. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...fields in order to provide useful solutions to complex challenges. As a cross-disciplinary researcher,...Many of the challenges in science today are too complex for any single discipline to solve them on their... disciplines leading the way, the future of complementing published research with video overviews was ...may never access online journals or even use a computer.  Currently, two of the more popular biology videos...derived from research. The platform uses video to complement traditional print journals and expand our ability...

  7. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...in the targeted brain region after stimulation compared to controls without Chr2. c-Fos is a reliable ... to proceed with your behavioral experiments. Comparison of c-fos amongst animals that receive different...Inhibition of neuronal activation is a little more complicated since basal neuronal activity in your brain region...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow...applying optogenetic tools derived from direct comparative analysis of microbial opsins." Nature methods...

  8. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...revolutionised the way we apprehend molecular complexes and is still a very useful tool.    Like a guitar... These issues can be overcome by choosing a complementary pair of fluorophores. To maximize the FRET signal...to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian...Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest fused between...especially when you investigate unknown molecular complexes. But it is a parameter that you have to keep in...

  9. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... of the policy comprised of numerous objections and concerns. Perhaps least compelling of these were those...to individual researchers. Yet accurate and comprehensible organization of data is essential for efficient...numbers of figures such that many have multiple, complex panels. PLOS, itself, has not finalized its definition...collections and interpretation of data, publishing complete data, rather than just a single projection of ...

  10. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...such as cancer since mice better recapitulate the complex interactions between cancer cells, therapeutic ...Scientists can make a new line of flies in only 6 weeks compared to months to make a transgenic mouse line. Over...be used to easily screen multiple therapeutic compounds in one experiment. Zebrafish (Danio rerio) Since...embryonic development studies as their embryos are completely transparent. Thus scientists can easily observe...model organisms, the C.elegans genome is also completely sequenced and over 60% of human genes have an...

  11. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... (Sandomenico et al., 2020). Figure 1: Comparison between the IgG antibody and scFv.   What...their lack of an Fc region. Disadvantages scFVs:  Compared to antibodies, scFvs tend to have lower affinities...intensity of a tagged protein. SunTag has two components: 1) a protein of interest that’s tagged with ....0000000000000145  Gąciarz A, Ruddock LW (2017) Complementarity determining regions and frameworks contribute...antib2020193  Khantasup K, Chantima W, Sangma C, Poomputsa K, Dharakul T (2015) Design and Generation of ...

  12. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...sword Microtubules are one of the most important components of the cytoskeleton. They provide cells with ...you’re interested in that other most-important component of the cytoskeleton – actin? Actin dynamics control...manipulating Rho GTPases based on BcLOV4. These single-component systems take advantage of BcLOV4’s light-induced...powerful enough, what about altering the chemical composition of the membrane itself? The Baskin Lab previously...K. H., & Chow, B. Y. (2022). Designing Single-Component Optogenetic Membrane Recruitment Systems: The ...

  13. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...for a Faculty Position" eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science... research is about and what you think you can accomplish. No matter how many times I say this, candidates...specific questions and experiments. Nobody EVER complains that a seminar was too easy to understand. Make...detail. Finally, state how successfully completing the research will advance the field, create a...yourself to me by email or in person. If you feel compelled to repay me in some way, I encourage you to help...

  14. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...size, while SSC values depend on the structural complexity inside the cell or on its surface. Bear in mind...on the cell’s size, morphology, and structural complexity.   Height, Width, and Area Within FSC and...each coming with a different size and structural complexity. After several steps of tissue processing prior... for a single cell gate. However, make sure to compare H and A (or W and A).   Live/Dead Gating Having...cells stain positively for both markers. When comparing two markers simultaneously, we commonly describe...

  15. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...lots of hustle and bustle, but the lab was very competitive and not very communal. That didn’t suit my collaborative...choosing a training lab, I strongly suggest you complete a work rotation in the lab if at all possible.... of effort, intimidating use of discipline or competence procedures Threats to personal standing – including...atmosphere makes you successful. Collaborative Competitive Papers have multiple authors One author plus...choose a grad school or postdoc lab. Having a comparison can be very helpful in uncovering strengths and...

  16. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ... a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold...yield, brightness and improved maturation time compared to similar orange fluorescent proteins. In their...more than 2-fold brighter signal from Antares as compared to Fluc in this model. The construct for Antares...transfer of energy from one fluorescent protein to a compatible fluorescent protein. FRET can be measured by ...fusion protein called CalFlux VNT. CalFlux VNT is composed of Venus fluorescent protein, the Troponin C Ca...

  17. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...programs. The software has two components - a database of pre-compiled register designs and a search function...sites, promoters, genes, terminators, etc) are composed. Fear not – the state machine design space is ...circuits in general would benefit from a more comprehensive characterization of the recombinases at our ...the Lu Lab at MIT. He is broadly interested in biocomputing and biological circuits.     References 1. N...

  18. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics to treat neurodegenerative...law. He said, “you know, the patent lawyer at my company seems to really like what she does.” I thought ...patent counsel and ran a group at two fairly large companies, Genzyme and Biogen. They’re very different jobs...corporate work, and negotiating contracts with other companies. It's one of those "every day is something different...

  19. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ..., in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment... cells, an important immune cell type, in more complex and physiological microenvironments. These screens...immunogenic, a necessity for in vivo studies; (3) is compatible with single gene KO and pooled screening approaches...facilitate the isolation of modified cells; and (5) is compatible with the introduction of unique molecular identifiers...

  20. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...Beth Kenkel To complement our RNA Extraction Without A Kit blog post, we've compiled five methods for...methods to do this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about ...features two mechanisms that cause plasmids to be incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow...
Showing: 301 - 320 of 798 results