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We narrowed to 313 results for: POR C

Showing: 301 - 313 of 313 results

  1. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...in fewer cells being considered for the analysis. (C) A more liberal gate allows more cells to be considered...signals. For this, you’ll need to gate based on the proportion between H and A (or W and A) within the same ... just as fine (Figure 4). As H and A scale in proportion (while H and W do not; see Figure 2), single ...                                                                                                         B.    Figure 4: (A) Using the proportional relationship between FSC-H and FSC-A we can ...

  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason, Lynn C., et al. "Recombineering...merely co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second...

  3. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...3):937-948. doi: 10.1016/j.ymthe.2020.11.009  Guo C, Ma X, Gao F, Guo Y. Off-target effects in CRISPR/...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good... score their probability of off-target events: CRISPOR, Cas-OFFinder, CCTop, and many more. While such...

  4. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...composed of Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase. CalFlux...has dubbed these fusions LumiFluor Reporters. The LumiFluor reporter constructs were designed to be packaged... The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...share your constructs from lab to lab. Promega supports the Addgene mission statement: Accelerate research...(FLuc) and Renilla (RLuc) luciferase. This is important not so much for how bright you can make a reaction...couples NLuc to fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins...host cells. The fluorescent moiety of the BRET reporter was used to sort successfully infected/expressing...

  5. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...10.1016/j.cell.2013.04.025 Zhang X, Liang P, Ding C, Zhang Z, Zhou J, Xie X, Huang R, Sun Y, Sun H, Zhang...enrich and select for cells that had successfully incorporated the desired mutation, and then derive mice from...handling embryos ex vivo, the components can be electroporated into the oviduct of pregnant females, a technique...gRNA, Cas9, and donor oligonucleotide can be electroporated into the mouse zygote (Qin et al., 2015).  ... it a single nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with... microinjectionist, who has the strategically important job of delivering the payload! Last but not least...-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease. Genetics 200:423–430 . https:/...

  6. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...repository 49:45 0 1:18:45 - Reagent Development - Steven C. Almo, Institute for Protein Innovation  1:18:45 -...findings can be predictably applied to future work. Importantly, no one should expect that every study should...of reproducibility for preclinical studies, as reported by drug companies attempting to apply the findings...and use computer based analysis techniques, an important component of reproducibility is making sure that...

  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...4354 . https://doi.org/10.1073/pnas.76.9.4350 Yang P-C, Mahmood T (2012) Western blot: Technique, theory,...while less protein leads to a thinner band. It is important to note, however, that you must include a loading...

  8. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...measure this effect, known as covariance. This is the “C” that differentiates these tests from ANOVA/MANOVAs...most effective and accurate way possible it’s important to select the right test for the job (and, uh,...

  9. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ... Y, Miki T, Fujioka R, Uehara R, Tomioka A, Obuse C, Kubo M, Hiwatashi Y, Goshima G (2012) An Inducible...fluorescent reporter, directly couple silencing of your gene target to silencing of the reporter. This is...application of long or short dsRNA. However, it is important to note that naked and unmodified dsRNA elicits... Together, this excellent work highlights the importance of using orthogonal methods to confidently conclude...fusing your gene target sequence to a fluorescent reporter sequence (discussed below). Examples of these ...genome, whereas shRNAs are exogenously derived. Importantly, instead of the assortment of siRNAs generated...contains a target sequence against the fluorescent reporter in an inverted repeat orientation. Therefore, ...

  10. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ... my senior year isolating pheromones from various C. elegans mutants by myself, I would have slowly gone...orchestrate the formation of high-functioning-but-temporary teams that have a specific purpose and finite ...year, or a few months, or even a few weeks. The important thing to remember is that every team should have...when I do come across one. Also, remember, one important feature that all projects have in common - and... What does a good team need? Besides that all-important sense of purpose, there are many more things necessary... function is certainly necessary, but equally important is making sure all team members understand what...their responsibilities are. That’s a subtle but important distinction. If I invite someone to be on a project...

  11. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...920-931 (2005). PubMed PMID: 16299477. 3. Eggeling C, Willig KI, Sahl SJ, Hell SW. Lens-based fluorescence...Harvard University. No matter whether you are a sports photographer at the Super Bowl, a medical technologist...the first fluorescence microscopy technique to incorporate optical sectioning. Optical sectioning refers...their fast movements. Depending on the sample, a temporal resolution on the millisecond scale may be needed...plane of excitation light that is thinner than the portion of the sample that is in focus. This technique ...resolution due to the lack of a requirement for high temporal imaging speeds. Standard fluorescence widefield...sectioning, imaging over large distances and high temporal resolution. Traditionally, this type of sample...

  12. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals an essential role... methods allowed immunologists to develop a spatiotemporal view of an immune response, but the study of...transgenic mice expressing fluorescent protein reporter constructs in the immune lineage. By specifically...than the standard Ti:Sapphire laser. It is also important to finely tune the excitation wavelength as it...using 2-photon microscopy as this hasn't been well reported for many fluorescent proteins. Additional fluorescent...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...doi.org/10.1371/journal.pone.0197185. Chamness, James C., et al. “An Extensible Vector Toolkit and Parts Library...sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014). This kit is a great starter...upon (its ‘basics’ MoClo plant kit buddy). It is important to understand your experimental needs before choosing...into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing of sgRNAs...basic biology of and gene regulation in the opportunistic pathogen Acinetobacter baumannii (Brychcy et...used successfully for protein localization and transport studies in HEK cells, recombinant protein purification...Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports, vol. 9, no. 1, Springer Science and Business Media...
Showing: 301 - 313 of 313 results