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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 552 results for: PPL;

Showing: 301 - 320 of 552 results

  1. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...connection you made at the job fair when you formally apply for the position. This post on Business Insider ...those relationships. Then when you are ready to apply for your dream job, you’ll be in the best possible...

  2. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    .... Given this large sum, one might expect that applicants would work for months to hone a chalk talk. Yet...of common questions, check out this eBook about applying for faculty positions. You need to practice with...

  3. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking...thinking” in the position to which she was applying. I probably did not respond to her question as well then...

  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...for microglia research and broader therapeutic applications.  Find microglia-targeting AAV plasmids here...reconstituting coding sequences for a range of applications, including CRISPR activation by dCas9-VPR, prime...

  5. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...ultrasound imaging of nanoscale acoustic biomolecules. Appl. Phys. Lett. 110, 073704 (2017). Hurt, R. C. et ...

  7. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...They generated variants optimized for different applications (v82 has higher affinity for glutamate but slower...lower binding affinities to broaden the range of applications. Image reproduced from Torres Cabán et al. 2022...

  8. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...world, there is a myriad of tools this could be applied to. When it comes to altering gene expression, ...is an umbrella term for a number of different applications that involve different types of small RNA molecules...

  9. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef...

  10. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...indiscriminate ssDNA cleavage activity which cleaves the supplied fluorescently labelled ssDNA substrates. Upon ...fluorescence can be visualised. Another approach was to apply the same concept to lateral flow strips that contain...

  11. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...earlier analogy, no amount of shouting will make an apple fall up. As scientist and politician Rush Holt said...are split along party lines (18). Feeling like an apple will fall up instead of down when dropped will not...

  12. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...Scarless Modification of Proteobacterial Genomes." Applied and environmental microbiology (2019): AEM-00990...chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015): 5103-5114. PubMed...

  14. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature...

  15. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...sequencing. While this may be unnecessary for many applications, it is the only comprehensive method available...you’ve tried everything listed here. For some applications, this is ok, but it still must be controlled...

  16. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...choosing a lab, but almost all of these guidelines can apply to any workplace or job. It amazes me when the most... Is the work (topic and/or technical aspects) applicable to industry if this path interests you  (e.g....

  17. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...Franklin L., et al. "Revisiting phage therapy: new applications for old resources." Trends in microbiology 23.4...functional genomic and metagenomic screening." Applied and environmental microbiology 74.5 (2008): 1649...

  18. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...extensively characterized and validated, with the application data from IPI and other labs easily viewed in...2021). Directed Evolution: Methodologies and applications. Chemical Reviews, 121(20), 12384–12444. https...

  20. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...sequencing is laborious, time consuming and cannot be applied to heterogenous populations (Bell et al. 2014)....Genotyping Options Technique Detection Limit Application Advantages Disadvantages GUIDE-Seq 0.1% Cell-based...
Showing: 301 - 320 of 552 results