We narrowed to 372 results for: RELA

1. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   Published

   Dec. 3, 2019, 11:38 a.m.

   ...helpful information about the subject, link to related guides and protocols, and provide easy to sort ...

2. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   Published

   May 30, 2019, 12:44 p.m.

   ...of the growth factor IGF2 open reading frame to relax imprinting (Zhang et al., 2017). This upregulates...

3. Negative Can Be Positive: Open AAV Data with Addgene

   Type

   Blog Post

   Published

   Sept. 6, 2022, 1:15 p.m.

   ... AAV questions every year on a range of issues related to AAV vectors, many from people trying to answer...

4. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   Published

   Aug. 30, 2022, 1:15 p.m.

   ...maintaining the structure of your samples, the relationships between cellular components, and the accessibility...

5. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   Published

   July 27, 2023, 1:15 p.m.

   ...The genome of viral tools are often not virology related at all, and you will instead get expression of ...

6. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   Published

   July 31, 2018, 12:55 p.m.

   ...X-linked retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing and some will ...

7. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ...is recommended you check your cell lines for HR-related mutations before attempting knock-ins by HDR. ...

8. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ...easiest switch from fluorescent proteins given the relative simplicity of the system and the availability ...

9. Lentiviral Vector Uses and Overview

   Type

   Blog Post

   Published

   May 19, 2016, 2:30 p.m.

   ... deliver a large amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise...

10. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ... necessary control elements for each would be relatively large. Cloning the viral genes in this plasmid...

11. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...future edits. In contrast, since Cpf1 cleaves relatively far away from the PAM, NHEJ might retain the ...

12. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    Published

    Aug. 20, 2015, 2:30 p.m.

    ...close-out a grant. Grant professionals also build relationships with foundations and government grantmakers....

13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ... of the reasons why we have only three biology-related Nobel prizes, out of which only one was done in...

14. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ...neuroscience to reveal insights into the complex relationship between genes, neurons, and behavior. There ...

15. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search to a ~4.5 Mbp region...

16. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    Published

    Nov. 12, 2020, 2:15 p.m.

    ...studies have shown that translational efficiency is correlated with codon bias across all endogenous genes in...

17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...binding specificity: SpCas9 variant Mutations (relative to SpCas9) PAM sequence D1135E variant ...

18. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...Class 2, candidate 1, 2, or 3). C2c1 and C2c3 are related to Cpf1, but they require both a tracrRNA and crRNA...

19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    Published

    July 8, 2025, 1:15 p.m.

    ...in what position do you want your gene to be in relation to the rest of the genes? And do you want it to...

20. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ...Like other transversion editors, this editor had relatively high indel rates ranging from 4.7% to 30% at ...