Skip to main content
Addgene
Showing: 301 - 320 of 619 results
  1. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...assembled or theoretical — it does not have to be entirely verified by sequencing. Upload any support files...easier for other scientists to find information related to your plasmid. It is optional to include the ...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations...
  2. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...inhibited by the unfolding of LOV, allowing the release of Cas9. A noteworthy application of the CASANOVA...in U2OS cells. Upon light activation, dCas9 is released within minutes and red foci formed 20-40 min later...Niopek D (2020) Optogenetics and CRISPR: A New Relationship Built to Last. In: Methods in Molecular Biology...
  3. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ...Videos can save researchers time by quickly relaying key points about an unfamiliar topic or lab process...page is to make it easier for you to find content relevant to your research and career goals, or to answer...
  4. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ...Cervical Cancer is the leading cause of cancer related morbidity and death among females in Ghana and ...for cervical cancer development and also better correlate the presence of certain HPV subtypes with risk...
  5. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ... is in a top-tier publication. Accessibility is surely also behind the increase in citations for older...scientific discourse has increasingly moved online, surely times have changed. Altmetrics.org calls to expand...
  6. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ... may need to sonicate your lysates in order to release the proteins from their binding. If you’re seeing...technical replicate (ie, leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget...migrate…so don’t try eliminating one component entirely. If you are trying to transfer proteins of vastly...
  7. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...protein domains from non-vertebrate systems. Preliminary testing indicated that inntags IT5, IT6, and ...Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells."...
  8. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...beneficial effects on the brain,” Ruas said in a press release. “We actually found the opposite: well-trained ...doi: 0.1016/j.cmet.2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...
  9. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...those optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to 20. A ...modifications in the genome,” Zhang said in MIT’s press release. In an accompanying Nature News & Views Andreas...
  10. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ... I actually started at a later date and worked entirely virtually. Stepping into this new virtual world... departments of the company. I asked questions relating to their roles at Addgene. I had a great time ...
  11. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...up hobbies (for her its gardening) that help you relax and enjoy life. “I love academics because it offers...other's accomplishments? Any tips on making time to relax and/or enjoy time away from the bench? We would ...
  12. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...mentors and mentees have fulfilling, productive relationships. I will be sharing what I’ve learned along the...many had examples of very productive mentoring relationships with advisors and others who helped shape their...
  13. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...thinking about how I could fill this time. I was relieved to find out that the two hours were split up between...gives a presentation on what they do and how it relates to other teams at the company. This provides new...
  14. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...inhibitory feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors....2019.02.037 Kagiampaki, Z., Rohner, V., Kiss, C., Curreli, S., Dieter, A., Wilhelm, M., Harada, M., Duss,...
  15. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...  It was with a sense of relief that I attended the amazing, postdoc-driven Future of Research Conference... all of the research centers in Boston.  I was relieved because it finally feels to me like the postdocs...
  16. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ... for two-color imaging in animals. The approach relies on ultrasound waves as opposed to light to produce...when in doubt, read the methods sections of the relevant papers, and then read them again. Most of the ...
  17. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that..., Susanna, Tenisha, Thomas, Tony, Tracy, Tyler, Tyrel, Wogenie, and Xuan Additional Resources on the Addgene...
Showing: 301 - 320 of 619 results