We narrowed to 631 results for: Rel

...proper controls are invariably inconclusive and unreliable. Proper controls provide the constant variables...Regardless, the amount of reporter protein activity correlates to both the amount of DNA transfected into the.... Replicate controls: technical and biological Reliable results are reproducible from properly controlled...

...can do and, in this way, science has always been related to government and, consequently, politics. More...Inclusion of data informing policy specifically related to the following subjects: Climate change Vaccines...Healthcare Science education To make scientists relatable (17, 20). This is an effort to show the general...

... questions in the sections below. Mentoring relationships can help identify your priorities and needs,...stay around? Is it too long or do they leave prematurely? Do lab alumni stay in the same field taking...Does the PI keep up on the literature and pass on relevant papers? Will you have access to information from...

...only a small number of glutamate molecules are released in each synaptic event and they are rapidly taken...Institute for Protein Innovation (IPI) recently released a set of highly-specific recombinant antibodies...efficiency and lower indel formation than methods relying on homology-directed repair (HDR), and insertion...

...for dinosaurs wreaking havoc in a theme park.  Released in 1997, GATTACA is an American SciFi film starring... breaks (DSBs) in the genome. These technologies rely on the cellular DNA repair machinery, which can ...the adenine base editor (ABE) enzyme in 2017. ABEs rely on a similar strategy as CBE but use a ssDNA adenosine...

...Biochemist, we geneticists are a lazy bunch who love to rely on the awesome power of genetic selection. Knock-in...recovery of these edits. Initial work in C. elegans relied on selective markers such as drug resistance (Chen...geneticist, I developed a co-selection approach relying on repair of a conditional-lethal mutation to identify...

... give advice and guidance to those that ask. Relationship building is key in science policy. Luckily, ...happy hours that you can attend to help build relationships.  Reach out to a science policy professional..., communications, diplomacy and international relations, advocacy, and writing legislation (see Figure...

...ligase, conditions and suitable probes, LDR can reliably detect single-base differences. The original creators...interrogation of multiple potential SNP sites (15). The related LCR technique uses two sets of probes to achieve...DNA library preparation. However, when a method relies on accurate ligation of nicks lacking gaps or mismatched...

...Many intracellular sensors of events like calcium release have been made using fluorescent resonance energy...biosensors, the intensity of the FRET signal can be correlated to specific concentrations of biological molecules...et al. (7) wished to trigger intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring...

...vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic liposome) or electrical...Quickly after transfection (4-8h), cells start releasing viral particles into the culture supernatant and...viral capsid antigen p24, the FACS dosing method correlates viral titre to the number of cells transduced...

...2011. 12(2): p. 79-89. PubMed PMID: 21252997. 37. Merrell, A.J. and B.Z. Stanger, Adult cell plasticity in...2016. 17(7): p. 413-25. PubMed PMID: 26979497. 38. Thorel, F., et al., Conversion of adult pancreatic alpha-cells...Models for Disease & Open Science: Interview with Darrell Kotton Four Factors that Differentiate the Stem...

...inhibited by the unfolding of LOV, allowing the release of Cas9. A noteworthy application of the CASANOVA...in U2OS cells. Upon light activation, dCas9 is released within minutes and red foci formed 20-40 min later...Niopek D (2020) Optogenetics and CRISPR: A New Relationship Built to Last. In: Methods in Molecular Biology...

...Videos can save researchers time by quickly relaying key points about an unfamiliar topic or lab process...page is to make it easier for you to find content relevant to your research and career goals, or to answer...

...Cervical Cancer is the leading cause of cancer related morbidity and death among females in Ghana and ...for cervical cancer development and also better correlate the presence of certain HPV subtypes with risk...

... is in a top-tier publication. Accessibility is surely also behind the increase in citations for older...scientific discourse has increasingly moved online, surely times have changed. Altmetrics.org calls to expand...

... may need to sonicate your lysates in order to release the proteins from their binding. If you’re seeing...technical replicate (ie, leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget...migrate…so don’t try eliminating one component entirely. If you are trying to transfer proteins of vastly...

...protein domains from non-vertebrate systems. Preliminary testing indicated that inntags IT5, IT6, and ...Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells."...

...may help you identify relevant protocols and experimental data Find other related plasmids in the "Associated...

...beneficial effects on the brain,” Ruas said in a press release. “We actually found the opposite: well-trained ...doi: 0.1016/j.cmet.2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...

...those optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to 20. A ...modifications in the genome,” Zhang said in MIT’s press release. In an accompanying Nature News & Views Andreas...