Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 809 results for: TES

Showing: 301 - 320 of 809 results

  1. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...complex, a model may be most effective. Once drug candidates are isolated, they can be validated for specificity...even be used to generate patient mutations and then test both individual and combinatorial drugs on for effectiveness...and validate the hit compound. The standard is to test the drug on models which are genetically proficient... can save time and money to make sure quality candidates are selected.      CRISPR aids in multiple...

  2. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    Published
    Oct. 18, 2017, 1 p.m.
    ... at eScience Labs, an educational company that creates hands-on science lab kits and digital curricula..., how to design good lesson plans, how to design test questions, and how to design lectures. I really ...happenstance. It was on Indeed or one of the job search sites. It's  a rare trek these days to go in cold turkey...

  3. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    Published
    Oct. 10, 2019, 12:41 p.m.
    ....  To develop HA frankenbody, the Stasevich lab tested several scFvs scaffolds previously proven to fold...frankenbody is that it remains bound to HA epitopes for minutes in living cells, meaning that scientists can track..., D. (1972). Localization of antibody-combining sites within the variable portions of heavy and light ... the National Academy of Sciences of the United States of America, 69(9), 2659–2662. PubMed PMID:4560694...

  4. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...(pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium... for imaging of small neuronal processes like dendrites and axons. Find the 7b variant of jGCaMP7 in AAV1...is the primary inhibitory neurotransmitter in vertebrates, but methods for directly imaging GABA have been...pAAV.hSyn-FLEX.iGABASnFR.F102G) is noticeably dimmer than iGABASnFR, and accumulates in the endoplasmic reticulum, but exhibits larger...

  5. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...Addgene is thrilled to announce the launch of our latest community resource: Recombinant Antibodies! In ...which the recombinant antibodies are derived were tested for specificity in knock-out lines or animals.  ...include information about immunofluorescence, conjugates, isotypes, and more. How You Can Participate ... represent the official views of the National Institutes of Health....

  6. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...sessions. “At big conferences, you could walk 20 minutes between one session to another,” Bachle says. “...note-taking doc where participants could share their notes together. Figure 1: Can you draw Spongebob...and making sure you stay connected. If possible, test out the technology before the conference begins....after the meeting, I received a lot of LinkedIn invites.”Bachle pointed out that networking at virtual ...

  7. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...was locked down, a South African company wanted to test if a type of antibodies from an Andean mammal called...preprint repository BioRxiv. This example clearly illustrates the power of collaboration, which despite the...volunteers are extremely diverse. Some of them are undergraduates or PhD students who are just starting their...trial-and-error. A recommendation for the correct statistical test can save a retraction. Today, collaborations are...

  8. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...during Zoom lab meetings to chip in ideas and take minutes that can be accessed by anyone at any time. This... be written by several authors from different institutes, disciplines, and cultural backgrounds.” This...simply moves away from the desk to switch off a few minutes. The host could also set aside Breakout Rooms to... allowed with a different host each time that moderates conversations. This can help team building and...

  9. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds,...can be counteracted by growing the cells on solid plates, where each transformant forms its own colony, ...solid media. The colonies are then scraped off the plates and pelleted for DNA extraction. Prepare library...using both pre- and post-amplification DNA and generates PCR sequencing products from both. These products...

  10. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...dissociation rates and discovered that the catalytically active form of the nuclease dissociates very slowly...our surprise, we measured identical dissociation rates for catalytically inactive dCas9 molecules. We followed...precisely spaced region allowed sequence replacement rates of approximately 1%. This is by no means a large...

  11. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...is a challenge. To help keep you abreast of the latest fluorescent proteins, I maintain an interactive...match to the filter set but the excitation filter excites more of the peak of the TagRFP excitation and the...to be extremely bright. In S. cerevisiae, we've tested a number of green and red fluorescent proteins ...optimal for all organisms and that if you want the brightest signal, you may need to try several proteins in...

  12. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ... want to buy, it is worthwhile to test-drive a couple of candidates. Before you buy a new microscope, ...summarizes properties of fluorescent proteins on websites. To make the best choice, the fluorescent protein...than theoretical brightness for selecting the brightest fluorescent protein. Theoretical brightness Two...

  13. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...or PolyPhen to approach this challenge, using attributes of cancer variants such as evolutionary conservation...have been two huge challenges preventing us from testing this hypothesis: There was no large scale, broadly...whether gene-agnostic bioassays could accurately test for molecular signatures of variant function and...the second major challenge, we developed assays to test large numbers of cancer variants across a large ...

  14. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ... PAM specificity, allowing for the targeting of sites that were previously unavailable. Figure ... with a broader PAM specificity can target more sites. Created with BioRender.com. Specificity Specificity...variants that were able to target different PAM sites (NGG, NGAN, NGNG, NGAG, and NGCG). Since then, the...eSpCas9 and SpCas9-HF across a number of different sites. Using a combined random and directed mutagenesis...

  15. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...cleavage, and suppresses neuronal transmission within minutes. Constructs using the photodimerizer system CRY2...Escherichia coli and Salmonella Typhimurium. This work demonstrates engineered gas vesicles as the first reporter...is regulated in the 3’ UTR by four miRNA target sites and thereby serves as a sensor of miRNA presence...population, or identify differentiation and disease states. This collection of reporter plasmids is amenable...

  16. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ... 49,000 times! Their long-lasting popularity illustrates the utility these tools have in many different...pyogenes with an invariant sgRNA scaffold and cloning sites for inserting the guide sequence (pSpCas9(BB)). ...notice the “V2.0” at the end of this plasmid which indicates that there was a prior version of this plasmid...retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev...

  17. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...fantastic K12 education initiatives like BiteScis (https://bitescis.org/) and amazing graduate student writing...writing collaboratives like Oceanbites (https://oceanbites.org/) have already grown out of our workshops...

  18. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...These small, but mighty type VI-D enzymes are the latest tools in the transcriptome engineering toolbox....sequence! In bacteria, Cas13d-mediated cleavage promotes collateral cleavage of other RNAs. As with other...efficiency varies widely across guide RNAs, each guide tested with CasRx yielded >80% knockdown. It seems that...maintains its RNA-binding properties, Konermann et al. tested its ability to manipulate RNA species through exon...

  19. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...control AAV in smaller aliquots of 20ul for serotype testing to help you pick the best AAV for your experiment...GABA-ergic interneurons (83895). See our Serotype Testing AAV inventory for the entire collection! Plasmid...activated, this DREADD acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor...functional Cre enzyme that induces recombination of lox sites. This system takes advantage of the extensive supply...

  20. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...none of these constructs have been functionally tested by Ginkgo Bioworks or Addgene. If you used plasmids...combinations of WT, codon optimized, untagged, cleavage sites (thrombin and TEV), and C-terminal tags (StrepII...first pass QC. The lab team manually transforms, innoculates, and performs DNA minipreps for small batches...mutation.  Once the samples arrive, each group of plates will have select samples prepared and screened ...
Showing: 301 - 320 of 809 results