...an entire classification scheme, known as the Baltimore replication classes, has been developed around...around how they replicate (Figure 2). Figure 2: Baltimore replication classes. Image credit: Louten, 2016...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...And now that you know what viral vectors are, it’s time to dive into how they’re used! We have a growing...

...outstanding material transfer agreement (MTA) turnaround times: less than two days for material requests and less...agreement must be put in place. And subsequently, every time that material is requested, a material transfer ...By default, your TTO must sign an agreement each time a material is being requested or deposited. But,...future requests/deposits. This reduces the processing time of request MTAs and deposit agreements to basically...

...in utero electroporation. Then at your preferred time points, sacrifice the animals and fix the samples...AAVs, it typically takes a few weeks to achieve optimal expression of XFPs. Any longer and the cells may...concentrations or AAV titer for the best results. The timing of sacrifice when using Tetbow AAVs - You will ...Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016): 2718...

...individuals for green cards because of the cost, long timeline and uncertainty associated with filing a permanent... a series of stages to refine and build skills overtime for success. Many people will have different roles... Ph.D. trained scientist. If the candidate is ultimately looking for a role in a different functional ...must be named in the media report (NPR, New York Times, respected online sources) and the media should ...must show that your company employs at least 3 full-time people engaged in researcher activities. The EB-...

...Use the Viral Service Tips for 1st Time CRIPSR User (By a 1st Time CRISPR User) Tips for Titering Your...from 7.4 to 6.8). It’s possible that a longer vent time would be sufficient to return the sample back to...handling recommendations Not all O-rings function optimally at -80°C. For samples that are shipped on dry ...

...a high titer Cas9 lentivirus prep takes a bit of time and effort that, for many groups, may not be worthwhile...enough lentivirus for you to both perform an optimization-scale infection and at least one screening-scale... robust. What you don't need to do is spend your time troubleshooting reagent production, because that's...make new lentiviral preps on your own, fear not - anytime you order ready-to-use lentivirus from Addgene,...

...repository, they remain accessible during these times. Centralized reagent repositories, also known as...access to validated reagents, especially during a time when these materials may not be available from their...Addgene and has already been requested dozens of times in 2 weeks. This is a striking example of the contributions...Microbiology 14:299–303 . https://doi.org/10.1016/j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol...

...visit   Design and communicate a research plan Your time at the new research site is limited, and we all ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...research and teach you new techniques. Show up on time (don’t make your bench mentor wait on you!), make...amazing career opportunity, but also a really good time! If it sounds right for you, start thinking outside...

...have been able to study the effects of various stimuli on global translation, but a visual technique to...by tracking the translation of a single mRNA over time. Both are helping researchers explore the complexity...that translates the mRNA. To permit tracking over time, Yan et al. devised a system that labels both mRNAs...fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the SunTag sites on ...

..., new Cas9 variants are being discovered all the time and the requirements of these different systems ...' NAAAAC  The synthetic guide RNA or gRNA (sometimes sgRNA) In the native Type II CRISPR/Cas system...direct repeat, combined with its adjacent spacer, ultimately encodes a single crRNA. The direct repeat regions...about all the different components of CRISPR, It's time to start thinking about how you can used them in...

... the hall, it is always a good idea to take some time to confirm that you are working with the correct...of all the plasmids we distribute. This method is time-intensive, so we recommend a variety of ways to ...enzyme. Consult the manufacturer’s manual for the optimal working conditions for each enzyme. Watch out for...The dyes also run at predicted sizes so you can estimate how far down the gel your bands have traveled ...

...enhanced signal of eNL allows their use for the first time in single molecule imaging. Nano-lanterns and your... al. “Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging.” Proc Natl Acad... variants of bright luminescent protein for real-time multicolour bioimaging.” Nat Commun. 7 (2016): 13718...Fluorescent Proteins Check Out Fluorescent Protein Timers Use Rosella to Study Autophagy Resources at Addgene.org...

... of these “variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we ...acid change based on maps of protein structure. Sometimes this provides a great deal of clarity. But, often... other cellular functions and phenotypes. Since time is of the essence in clinical settings, looking ...that could enable future clinicians to improve real time decisions when rare variants are discovered in clinical...

...world of precision genome editing. When it comes time to (hopefully) validate successful mutation of your... we found these assays frustrating to work with, time consuming, and minimally informative. In this blog...Instead of tracking the reaction progress as in real time PCR, each reaction partition is individually assessed... duplexed primer probe assays often used in real time PCR. These assays can be designed to detect either...

...synthetic biology is still in its infancy. A long timeline from transformation to testing and a lack of well-characterized...plants are being investigated on limited funding and time. While institutions that focus on creative problem...like a lot of specialized science, these tools sometimes have limited distribution. We hope that will change... 2014. Outside of the lab, Keira enjoys spending time with her husband and two Jack Russell Terriers. ...

...goals. These programs also serve as a great first-time mentoring experience. They make mentoring less mysterious... all group members to lead and learn at the same time Broadens the network of the participants Can be ...However, they find it awkward to ask someone for the time commitment of a formal mentoring relationship or...organizations as it brings a diversity of perspective. Sometimes it is helpful to find peers that share a similar...

...some of their time. There are many professional coaches who charge for their advice and time. Consider this... or who your new contact will connect you to. Sometimes, it is not who you know but who knows about you...share their wisdom and they might just not have the time. Thank them anyway and move on. If they say yes,...

...cells (iPSCs) has been around for 10 years. In that time, scientists have used almost all available approaches...relatively simple to implement and doesn’t require time-consuming production of virus. In theory this is...efficiencies and they tend to express for longer periods of time than traditional plasmids due to lower activation...therapeutic applications, but it can take a long time to reprogram cells. In Kim et al, cells were exposed...rigorously assesses pluripotency, but they also take less time to complete. Morphology is a simple and fast to ...

...allowed scientists to track multiple genomic loci over time using all the colors of the CRISPRainbow. ...telomere dynamics throughout the cell cycle. To optimize the signal to noise ratio of the system, they ...monitor the spatial relationship of the two genes over time, at a range of distances from 2-75 Mbp. Labeling...Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System.” Cell 155 (2013): 1379-1491...

... It’s time for another edition of “What’s New in CRISPR” - where we highlight a few of the newest CRISPR...recently shared some exciting plasmid tools to optimize prime editing systems. Engineered pegRNAs improve...upon knowledge of the biology of the system to optimize its outcomes. In their most recent work, the Liu...and improved editing efficiency. The lab also optimized the architecture and amino acid sequence of the...