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We narrowed to 743 results for: TRI

Showing: 301 - 320 of 743 results

  1. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...difficulty with NgAgo, but a few successes have trickled into the survey. In response to a bit of a Twitter...to make single base edits in DNA. The first, contributed by guest blogger Kristian Laursen from Cornell...mutated plasmid constructs. The second post was contributed by Addgene Scientist Mary Gearing and discusses...

  2. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...The following post was contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and...frameshift mutations. However these may require restriction fragment length polymorphism analysis, allele-specific... you to our guest bloggers! Daniel Bauer is a pediatric hematologist and principal investigator at Boston...

  3. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...Yeast. 1998 Jul;14(10):953-61. Note: Marcy Patrick contributed to the writing of this article....isolation of successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining...

  5. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... a repair template, using Gibson Assembly or restriction enzyme cloning. Streptomyces bacteria are more... presence of just a single organism, such as Clostridium difficile, could be targeted and preferentially...base editing in Streptomyces Some of the most industrially and clinically relevant bacterial species reside...CM, Ousterout DG (2020) In Vivo Targeting of Clostridioides difficile Using Phage-Delivered CRISPR-Cas3...

  6. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...Recycle plastic packaging Packaging is a huge contributor to the increase in plastic manufacturing, accounting...accept and to print a free shipping label. Recycle nitrile gloves Terracycle is a company that takes items... a box with the prepaid label and fill it with nitrile gloves, affix the prepaid label, and divert these...

  7. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...heavy restrictions on what I could and couldn’t do as an intern. I expected that these restrictions would...choose what gene to focus on. The gene choice had restrictions. It couldn’t be a Covid gene, a toxin, or an...

  8. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...NEBCutter2: For finding restriction enzyme cut sites Webcutter 2.0: For finding restriction enzyme cut sites ... different types of cloning methods, such as restriction enzyme cloning, Gibson assembly, or In-Fusion...

  9. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...Minigenomes - A Safe Way to Study Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of...leaving virulent components behind. Minigenomes are stripped down viral genomes containing only the sequences...fantastic depositors and guest writers. Your contributions make Addgene and the Addgene Blog useful resources...

  10. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ... This post was contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. ...general, I think that this is an (e.g. great contribution to the community because of..." This first couple...on-line repository (like GitHub, Zenodo, Figshare, Triad) while the wet-lab resources, like constructs or...

  11. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ... such as Stbl3s. ITR loss can be detected by restriction digest using enzymes, like SmaI, which have recognition...organism or tissue type-specific, which can help you restrict the expression of your transgene to a specific...result is a permanently flipped transgene. By restricting the availability of Cre, lox sites lets researchers...

  13. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...This post was contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School...thanks to Addgenies Pamela McA'Nulty and Zachary Tribbett for helping compile the data used in this work..., computing performance, and randomized control trials in innovation. Visit his website at: http://www.neil-t.com...

  14. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ... research community. At Addgene, we’ve helped distribute many of these technologies in plasmid form and...libraries. Please note, the pooled libraries we distribute in lentivirus format only deliver gRNA and therefore...like to thank all those who are allowing us to distribute their CRISPR tools as ready-to-use virus and,...

  15. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...developed in terms of the collection, storage, and distribution of the plasmids and their ability to identify...plasmids so scientists aren’t bogged down with distributing them and spending more time on research. What...LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S...

  16. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...sample. Practical brightness A protein with a high intrinsic brightness that does not fold well or cannot be...explanation of the assay is depicted in figure 1. The strict correlation between the fluorescent protein of ...

  17. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ... This post was contributed  by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad...works. But, those darn cancers have revealed many tricks up their sleeves and most patients still don’t ...or PolyPhen to approach this challenge, using attributes of cancer variants such as evolutionary conservation...

  18. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...These viruses have been distributed in over 200 packages to more than 20 countries. With this initial success...in transgenic animals, where cre expression is restricted to a particular cell type. The human synapsin...

  19. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...with biorender.com. ITR structure  WT ITRs are symmetrical 145-nucleotide sequences that flank the ends ...identification of non-dominant ITR sequences.  Restriction digests with the enzymes SmaI and SrfI can also...deletions and point mutations will go undetected by restriction digest methods.    Resources and references...

  20. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...your antibody.  At some point in your long and illustrious lab career, you will likely accidentally leave...Brightness Chart. Boston University Flow Core. Retrieved January 31, 2022, from https://www.bu.edu/flow-cytometry...overlap. Syracuse University Flow Core Facility. Retrieved January 31, 2022, from https://flowcore.syr.edu...
Showing: 301 - 320 of 743 results