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We narrowed to 519 results for: abo.2

Showing: 301 - 320 of 519 results

  1. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...tagging." Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and...takes a lot of time and energy, Martí Aldea and collaborators have created a set of “innocuous tags” (inntags...Pubmed PMID: 23435261. Resources at Addgene Read about other Protein Tags in our Plasmids 101 Series. Get...

  2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...sequence is unique compared to the rest of the genome.  2) The target is present immediately upstream of a Protospacer...utilized allowing for PAM flexibility. Fig. 2: Prime editor engaging target DNA. The reverse transcriptase... 101 Series Learn about the PAM Requirements of Different Cas9 variants Learn about CRISPR Pooled Library...

  3. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...whether the results include knockout data.    Figure 2: Search options in the Antibody Data Hub. Data reports... antibody worked under the conditions described above, and a short description of how the antibody performed...

  4. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...citizens enter into in order to protect their rights (2). Less formally, governments are the organizations...science education and literacy that this changes.  2) Lumping all scientists (and consequently their results... with a testable hypothesis - an educated guess about how some part of the world functions. Experiments...governments exist in reality and science is how we learn about reality. Formally, a government can be defined ... More concretely, scientists gather information about reality informing people and their governments on...people to rally for science, others are concerned about possible unintended consequences of a march. Three...Democrats more often than Republicans, the March is not about Democrats or Republicans (16). This is a Pro-Science...

  5. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...similar optical quality (magnification of 140X and 2 micron resolution). I have worked many hours in a ...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...After learning about using sound to visualize bacteria in the lab, I was excited to learn about creative approaches... at different ultrasound frequencies. Read more about ‘Using ultrasound to image bacteria in vivo: acoustic...Protein Resources and get started!  I learned a lot about creative ways to do science communication and outreach...outreach - if you'd like to learn more about Science communication check out these SciComm blog posts.... Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter  Resources ...

  6. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    Published
    July 7, 2017, 1:32 p.m.
    ...develop therapies to treat disease in the lung. Chapter 2: Balancing research with medical practice as an MD...promote scientific sharing. Listen to learn more about Darrell, his research, and his focus on open science...Medicine and in the Department of Pathology and Laboratory Medicine. He attends in the Medical Intensive...NIH-funded Principle Investigator in the Kotton Laboratory and is the founding Director of the Center for...Podcast.     Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids...Plasmids from the Kotton Lab Learn more about Stem Cells ...

  8. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ... on the future of the scientific endeavor October 2-3, 2014 Boston University Jacob Sleeper Auditorium...doing science in some form? There is a lot of talk about the state of scientist training in the US and around...

  9. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...SnapGene Viewer (Mac/PC) FinchTV(Mac/PC) Chromas (PC) 2. You should see individual, sharp and evenly spaced...and troubleshooting your DNA sequencing results Above (top right corner of this post) is an example of...

  10. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...Transgenesis." PLoS ONE. 8(12): e83043 (20 December 2013). 2. Carola Engler et al. "A One Pot, One Step, Precision...protein,” he says. “There is nothing you can do about it but be gentle. If it no longer works, just trash...

  11. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...phosphoinositides (Varnai and Balla, 2008) (Figure 2). RNA production can be detected by using a MS2 coat... 296.5569 (2002): 913-916. PubMed PMID: 11988576. 2. Tsien, Roger Y. "The green fluorescent protein." ...deleting residues. Only about 10 amino acids can be deleted from the C-terminus and about 5 from the N-terminus...cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel...based on cofactor binding proteins as described above. The smallest tags are peptides that are engineered...

  12. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...access to the results of federally-funded research. 2)      In June, the White House honored 13 “Champions...study probably doesn’t say as much about open access as it does about the state of peer review. 8)     ...University Open Data Access (YODA) Project, “a collaborative endeavor guided by aspirations to promote open...

  13. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...would label everything but the CD8+ T cells (Figure 2). Even better, we can use the same fluorophore for...gating on the negative population.     Figure 2: Dump gating labels all unwanted cell lineages with..., you may want to look at other methods. In the above cases, a good alternative is using a two-protein... region of the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...to a specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID ...cells will be ready for the spotlight.    Figure 2: Opto-RhoGEFs to control Rho GTPase activity. A) Schematic... Check out our Optogenetics Guide to learn more about how these modules work, and read on to learn how...to the cell membrane isn’t powerful enough, what about altering the chemical composition of the membrane...

  15. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set range in size ...to generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...PUF Assembly Kit Below you’ll find information about what these kits contain, how they can be used, and...encoded sensors enable real-time observation of metabolite production." Proceedings of the National Academy...

  16. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...fusion location, there are many choices to make. 2. Prime Editing: Adding Precision and Flexibility to...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. 5. Five Popular...

  17. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ... a template, copying the base edit. Figure 2: Cytidine deamination takes place on the free strand...SpCas9 base editors with editing windows as small as 1-2 nucleotides. To reduce off-target effects associated...formation. To further decrease indel formation 1.5-2 fold, the team fused bacteriophage protein Gam to ...Homology-Directed Repair Learn about base editing for plants Read about four base editor reporters Additional... The BE3 system increased editing frequency to above 30% for a variety of targets in human cell lines... Liu lab created BE4max and AncBE4max mentioned above, they also made an adenine base editor with improved...cell therapy applications. Dual base editors What about combining the function of both editors into one?...

  18. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...Almo, Institute for Protein Innovation  1:18:45 - 2:01:18 (end) - Panel Alex Tucker (Ginkgo Bioworks)...their curation of citable, linkable figures. Cause #2: Hidden “negative” data We all want to be able to ...easier to find information about research tools. BenchSci collates information about antibodies while FPbase...variety of additional resources and information about organizations promoting reproducibility in this ...that make it easier to share in-depth information about reagents, protocols, analysis tools, and more. Being...from a freezer. As you’ll see in Steve Almo’s talk about work at the Institute for Protein Innovation (IPI... talked to many researchers who have complaints about the unreliability of antibodies. IPI is working ...

  19. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...teaching and mentoring others (see more in Figure 2). Starting early demonstrates not only your interest...application process so you can learn more! Figure 2: The many ways to prepare for a science policy career...learn more about their career trajectory. If there’s one thing everyone loves, it’s talking about themselves...learn more about the field.  Read the CCST Science Policy Career Guide to learn more about science policy...Administration, I could never get one straight answer about what science policy even is! In this article, I ... the #scipol hashtag. Often there will be posts about virtual meet ups and happy hours that you can attend...solving one problem. It often requires thinking about a problem in a new way through the integration of...

  20. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...and trophies are awarded and coveted. I’ve only won 2 trophies over the years, which is either an indication...for the company, but also talking about sports, movies, and just about anything. As our organization grew... with our western blots. Thoughts about sharing Addgene is all about helping scientists share between ...culture of openness and trust that comes from caring about your colleagues on a personal level, and employees...often cite lunch as one of their favorite parts about working at Addgene. We’re a small non-profit, so...indication of the stiff competition or says something about my cooking.   This is a creative (and delicious)...for people in different labs to chat informally about their challenges, successes, and expertise. Who ...
Showing: 301 - 320 of 519 results