We narrowed to 711 results for: ang

...and numeracy on perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547... “motivated reasoning,” the catchall term for a range of neat little tricks our minds perform to avoid...

...to explore this role, how to seek out such an arrangement, and how to operate once in that position. What... plan as well. Making logistical and funding arrangements Visiting scientist positions take commitment...structured visiting program, you will have to make arrangements on your own. This will include, but is not limited...

... tremendously complicated entities with a large range of characteristics (color, brightness, photostability...FPbase.org FPbase is a project written in Python & Django, using a popular database management system (PostgreSQL...users to see when a given page has unmoderated changes and to view previous versions.  Currently, all ...

...MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas ...In addition to publishing in Science, Professor Zhang deposited the associated plasmids with Addgene. ...School of Management. His research focuses on a range of topics including biotech and research tools, ...

...can feel lost. Use this post to explore the wide range of careers available to scientists and open your...carry out in-depth research is applicable to a wide range of industries and is a highly desirable quality ...SAS, HTML, Matlab etc) are highly desirable in a range of roles, including: Science Reporter Technical...

... these proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury... Tsakanova to image cortical neurons and study changes in neural activity after ischemic brain injury.... diverse groups of scientists working in a wide range of research fields and geographical locations. In...

...This guest post was contributed by Ally Huang is a 4th year PhD student at MIT. While I had always enjoyed...make learning biology more fun – bit by bit. Ally Huang is a 4th year PhD student at MIT in Biological Engineering... can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A modular synthetic...

...protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1), some of the exciting...recombination. Find PhoCl plasmids at Addgene. Zhang W, et al. Nature Methods. 2017. PubMed PMID: 28288123... also provided a detailed protocol at Protocol Exchange. Plasmid maps can be found at Addgene and in Figure...flame depositor David Liu’s lab increase both the range and specificity of base editing. Kim et al. used...

...microbial SSAPs. Image from Wang, et al, 2022.  Find dCas9-SSAP plasmids here! Wang et al. Nat Cell Biol....target. Across all loci tested, error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error...

...heavy (blue) chains as driven by two CMV promoters (orange). Figure from Andrews, et. al., Elife  A limitation...step would be to validate your new construct by Sanger sequence analysis. But for this research, it was...express multiple subclasses of IgG antibody. Broad range secondary antibodies are commonly used in research...

...these reporters, editing typically results in a change in the fluorescent readout. In this blog we will...for easy screening of different gRNAs by simply changing the gRNA used when transfecting your cells.  A...GEmCherry2. The Denham lab made several other small changes creating 2 other versions but these did not surpass...

...related plasmids in our collection, we’ve relied on Sanger sequencing as a first pass QC. The lab team manually...that process is complete, QC scientists prepare Sanger reactions by mixing this isolated plasmid DNA with...sequencing primers, and hand deliver them to our Sanger provider. These results come back within 8-12 hours...

...bacteria out of their optimally suited temperature ranges and make them grow better at suboptimal ones, which... of Earth’s harshest environments: corrosive pH ranges, crushing pressures, near-melting temperatures,...discussions. Not only are such endeavors a welcome changeover from conventional academic settings, these are...

... the feature its characteristic T-shape. The arrangement of the B-B’ and C-C’ palindromic sequences determines... ITR sequences?  Next-generation sequencing and Sanger sequencing might fail to correctly read through...Chen, Y., Hu, S., Lee, W., Walsh, N., Iozza, K., Huang, N., Preston, G., Drouin, L. M., Jia, N., Deng, ...

...articulating why the results you've measured should change established theory. So, what can you do to ensure...you receive generalizes.  If someone suggests a change to a slide, think about whether the same principle...explain why the work is important and how it should change how the members of your audience think or act.   ...

...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome...tracrRNA? Yes No dsDNA cleavage Blunt end 5' overhang PAM sequence NGG TTTV PAM site preserved? ...Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease...

...enzymes function across a much broader temperature range than BioID, which only functions optimally at 37... Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way we detect viral outbreaks Article contributed...equipment. What’s more, detection is based on color changes to the assay paper, so no special equipment or ...

...the VSV-G envelope provides the widest tropism or range of cells a virus can infect. Note: While both lentiviruses...and γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (... vice versa. Envelope plasmids may be used interchangeably. Are γ-retroviruses replication incompetent...

...overlaps with the XbaI cut site (depicted as an orange line) because the restriction site is followed ...mutated plasmids which can later be verified by Sanger sequencing. How can I tell if my enzyme will cut...will be blocked by methylation and how that may change your expected digest results. If Dam methylation...

...upon themselves to teach me Python, a programming language favored by biologists. Almost as soon as I began...like a wet lab experiment I use the text editor TextWrangler to record the following, which are later printed...that further helps you keep track of your exact changes. Alternatively, you can simply make a note in your...