Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 814 results for: app

Showing: 301 - 320 of 814 results

  1. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...research. This year, we put out another call for applications. We had an overwhelming response and were excited...many creative uses of fluorescent proteins. We’re happy to announce the three winners of this year’s Michael...does that mean? Previous research shows that approximately 75% of all protein-coding pathogenic human variants...artificial leaf replica system. “This bottom-up approach will allow me to create a fully controllable and...

  2. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...erupted in controversy. Briefly, there are two overlapping, valid critiques being offered of He’s work: ...public involvement, research, and consensus can happen. We just have to look to the United Kingdom’s Nuffield... technique was feasible, three separate things happened nearly simultaneously: The Wellcome Trust funded...biosecurity, and the entire 2018 saga of “what’s happening in genetics this time?” She lives with her partner...

  3. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ... related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated ...fluorescence microscopy and FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will... the river back to the user.   At Addgene we're happy to be able to support diverse groups of scientists...collaborations. Many thanks to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and...

  4. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...in pairing with base editing and prime editing approaches. Recently, Xue Sherry Gao’s lab at Rice University...packaged into viral vectors for potential in vivo applications or multiplex CRISPR screening.   Using tRNAs...and efficient in plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided...construct DAP array for multiplex gene editing applications, we provide detailed manual and online design...

  5. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...spCas9 was the only Cas enzyme widely available and applied by researchers for gene targeting. Fast forward...properties, previously established uses, and intended applications. Within CasPEDIA, there is a classification ...multiplex ability Experimental guidelines and applications Protein structure and properties Links to ...you want to read literature on the less common applications of an enzyme for your non-traditional experiment...

  6. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...small, avoidable mistake. Or worse, when you're trapped in a pattern of small errors that significantly...of ways you could change your variables - your approach, your workflow, or your physical setup - to prevent...workflow can have a huge impact. Test different approaches, like whether it's better for you to use cold...resistant to. Just like any other experimental approach, use small, incremental changes to understand ...

  7. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ... with an intuitive user interface.   The team approached the issue from multiple angles: the overall user...of information so we were able to fine tune our approach much more than if we were dealing with general...” variations and synonyms of the same term that appear in our data into a single filter value. For example...our search engine to be so specialized that it appears to “understand” biology.  We hope you will give...

  8. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand...favorite FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if...got a useful tool, there’s likely someway you can apply it in neuroscience. AAV tools from Addgene are already...targeting genes to specific cell types in the brain, mapping neuronal networks, and selectively activating signaling...

  9. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ... are here to discuss the ins and outs of both approaches. Screens and selections The common goals The...lot in common. In fact, selections are often inappropriately categorized as screens! Why the confusion? ...details - and that’s where the differences in these approaches can be found. As an example, say you are considering...require there to be a culture condition that can apply enough pressure to select for variants of interest...

  10. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...review system. Peer review in traditional journals happens before the manuscript is accepted for publication...at the same time. Take your time picking the appropriate journal and explain in your cover letter why ...why you think your manuscript is appropriate for that journal. Argue with an editor or reviewer over their...ignored their comments, they will not be inclined to approve your paper. Rush your submission or revision. A...

  11. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...with the same genetic background and do an apples-to-apples comparison of all those mutations. If you ...take four plasmids out of the library in the appropriate combination and do a restriction enzyme digest...different genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare...

  12. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...previous post in this series described a practical approach to selecting a bright fluorescent protein. In ...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates...proteins. To avoid diffusion, the proteins are (i) trapped in microdroplets in a water/oil emulsion, (ii) ...

  13. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers...researchers to better understand the processes that happen inside the cell,” says Pedre Pérez. Figure...construction of the dual virus and the experimental approach. (B) CR-Cre mice demonstrate pain response due...

  14. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...systematically mutated it. Using a robotic picking approach to select cells with variant characteristics they...” vector cloning system - a Gateway compatible approach to simultaneously clone two genes-of-interest ...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...the photoswitchable proteins offers a powerful approach to control protein-protein interactions in cells...

  15. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, social...listing “Submitted” articles in their CV or grant applications. A number of organizations (such as ASAPbio)...and preprints are a great way to do that. We are happy to receive deposits of plasmids that have been described...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

  16. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...several methodologies currently available for approaching it. These include the traditional restriction...introduction.html demonstrating the principal behind the “swapping out” of ccdB with the investigator’s gene of interest...which can then be used for downstream cloning applications. While these two strains ultimately perform ...important to ensure that you choose the most appropriate method for your own experiments. Please check...

  17. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...high-throughput screening are vastly useful for mapping the combinatorial genetics that underlie complex...research questions. CombiGEM has been successfully applied to functionally characterize combinatorial gene...realization of a variety of perturbations and applications in functional genomics, cell reprogramming, ...He is particularly interested in developing and applying new technologies to interrogate and understand...

  18. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...mutated; elements that are difficult to sequence; approaches to confirm plasmid sequences without a reference...program called Geneious. Here, the raw reads are mapped against a reference sequence, if one was provided... determine if there are any notable QC issues. Mapping the raw NGS reads to a reference sequence can also...confirm their presence with confidence. If this approach does not resolve the identified differences, the...

  19. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods...to increased intracellular calcium. It has been applied to mark active populations of neurons in vivo to...expression and downstream monitoring. This targeted approach allows scientists to focus on specific cell populations...Vectors 101: Biosensors Viral Vectors 101: Viral Applications Viral Vectors 101: An Introduction to AAV ...

  20. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... the end goal, it is best to work with FDA/EMA approved drugs so that your results are viable for translation... be mitigated by using low doses and running appropriate controls (Jendryka et al., 2019), they have resulted...brain and some, such as varenicline, are already approved for clinical use. Varenicline has been further...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...
Showing: 301 - 320 of 814 results