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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 393 results for: ara-2

Showing: 301 - 320 of 393 results

  1. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...acceptable variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes...Cas12a’s ability to knock out multiple genes in parallel. Lastly, this Cas12a-based screening method was...gels. A: individually expressed proteins run in separate lanes. B: individually expressed and coexpressed...containing 150 or 250 kD proteins are also available separately. The proteins, which are purified via histidine...or Cas12a. Despite its small size, CasMINI is comparable in efficacy to dCas12a systems in both gene activation...

  2. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...closely mimicking the future application. In figure 2 we provide an example of the comparison of the practical...your selection on a small-scale head-to-head comparative study under conditions that best mimic the intended...practical brightness takes all application specific parameters into account, including the specs of your microscope...translated from the same open-reading frame and separated from the protein of interest by a 2A self-cleaving...microscopy (University of Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes...

  3. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...higher quenching of mScarlet emission. Figure 2: Ace-mScarlet is a fusion between the voltage-sensitive... was also between 1013 - 1014 vg/kg, a dosage comparable to those currently used in human gene therapy...response and brightness. They also do not spectrally separate from green sensors and blue-light-activated optogenetic...phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In the presence of red light (650 nm... PCB is no longer present, the fusion proteins separate, inactivating Cre. This is critical for reducing...

  4. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...responsible for that differentiation.   Fig. 2: Single cell RNA-seq clustering of cells transduced...road map, and what was known about previously characterized TFs, the Zhang group were able to classify unknown...studied TFs, this functional association of the uncharacterized TFs is a useful finding. Transcriptional program...

  5. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...cellular physiology.  https://doi.org/10.1002/jcp.30064 2. Barman et al. 2020. “A glance at genome editing with...with a dominant mutation in a gene that causes cataracts could be rescued by coinjection into zygotes of... explore its potential in mice with a dominant cataract disorder caused by a single copy of a gene known...

  6. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...and open a new conversation about GMOs.  Challenge #2: Technical obstacles to plant synthetic biology In...transformation to testing and a lack of well-characterized genetic tools make it challenging to engineer...understood. Additional work has also identified and characterized mechanisms and components of the circadian rhythm...

  7. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM Genes Dev. 2015 Jan 15;29(2):197...essential for the structure of paraspeckles. Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess...

  8. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...IgM, IgD, and IgG are deleted.     Figure 2: Isotype switching requires DNA recombination of the...isotype? An isotype determines several key characteristics of an antibody as well as the role it plays... regions result in several unique physical characteristics of these five isotypes, such as the number ...IgG4. IgE IgE antibodies help protect against parasites but also play a role in allergies. They are found...

  9. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...divided by the off-target activity. Figure 2: Specificity is the measure of off-target activity...experimental approaches. One example is CasX, characterized by the Oakes and Doudna Labs, which is both ...research and potentially treatments. Cas12b, characterized by the Zhang (Strecker et al., 2019) and Li ... relative to Cas9. As scientists continue to characterize CRISPR nucleases from a variety of sources, ...

  10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication ...from Pseudomonas aeruginosa to cleave 4 gRNAs separated by 28 base Csy4 recognition sites from a polycistronic...protoplasts. Each pair of gRNAs targeted genomic sites separated by 350-750 bp, allowing them to examine the frequency...different biological systems. Although Xie et. al characterized the system in plants, PTGs will likely work ...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...extraction solution, runs the protocol, then uses around 2–4 µl in each 50 µl PCR reaction. The gDNA extracted... early onset Parkinson Point mutations. Cell, 146(2), 318–331. https://doi.org/10.1016/j.cell.2011.06.019...system, developed by the Zhang lab, which has comparable efficiency to the optimized chimeric design but...for PCR and surveyor. Q15: I used DNA polymerase Takara Ex Taq™ DNA Polymerase for my genomic PCR, but ...successfully used Herc II or Kapa Hifi in PCR. Maybe this Takara enzyme is not very robust in this case for EMX1...processing of the crRNA) is also expressed from a separate promoter. In the pre-processing form, the crRNA...

  12. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag has been ...the need for an antibody. Tags can enable the separation of detection of a mutant protein vs. endogenous...

  14. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method...gradients and ultracentrifuged for up to 20 hours to separate the intact RNA from DNA and proteins. Although...and Sacchi showed that RNA could be effectively separated from DNA and proteins by a simple extraction protocol...thiocyanate solution. However, instead of RNA separation using cesium-chloride gradients, water-saturated...ultracentrifugation!), the layers of phenol and chloroform separate, and RNA is retained in the top, aqueous layer...

  15. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...still depolarize neurons at lower MOIs. Figure 2: ChRgers are delivered by intravenous injection into... “designer” channelrhodopsins with desired characteristics. The model was trained to predict if a channelrhodopsin...these remaining sequences were selected for characterization with patch clamp electrophysiology. All thirty...named ChRger1, ChRger2, and ChRger3, were then characterized further by expression in cultured neurons and...performed well in a tissue culture dish, that doesn’t guarantee their success in vivo. Therefore, the Arnold and...

  16. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...main decisions to make: 1. Which modification? and 2. Which viral vector should be used? Modifying gene...need to be given some time to adapt to a testing apparatus. General stress levels of the test animal may ...the humans’ audible spectrum. The experimental apparatus should be cleaned thoroughly between animals as...beginning. Variability source Standardization parameters Animal Strain, age, sex, housing conditions...order to maximize possible effects. Injection Parameters: The injection needle should be as thin as possible...

  17. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ..., but at a lower efficiency (~20%). Figure 2: Schematic of multicomponent assembly with SLIC. Multiple... other dNTPs. Once the vector and insert are (separately) digested to create 10–12 base “chewed back” ... T4 polymerase (left branch); by annealing two separate PCR products (middle branch); or by incomplete...

  18. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ... and transfer 100 µl of glycerol stock to each of 2 96-well microplates (identical copies, see image above...for processing several sequencing reactions in parallel. We have a few tricks to simplify the process....saves us the step of purifying the DNA and gives comparable success rates and sequence quality, which is ...

  19. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first...10.3389/fmicb.2020.607028  Riley, R., et al., Comparative genomics of biotechnologically important yeasts...
Showing: 301 - 320 of 393 results