We narrowed to 527 results for: set

...list of posters of interest, then you’ve already set yourself up for success. But sometimes, attendees...

... the same target. In the 1980s, Munro and Pelham set out to address this issue by developing generic strategies...

...future in under 200 pages is an impressive task to set oneself. As a scientist who enjoys learning a little...

...have the idea that if you don’t get your PhD you’re set up for failure. Listening to Kenkel’s story has helped...

...convex scaffold and are randomized with a defined set of different trinucleotides (corresponding to different...

... notice for the first meeting so that people can set aside time in their schedules and read the paper ...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out...

...chromoproteins are actually a subset of the fluorescent protein family. What sets them apart is the way they...

...allow for two sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus...protocol for MMEJ-mediated knock-in of a GFP-Puro cassette into a given locus, just upstream of a stop codon...microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary for...for knock-in: one on either side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies...of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA...creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific gRNA cleaves ...and 3’ microhomologies are added to the GFP-Puro cassette via PCR, and this construct is inserted into the...

... brightness is compared to that of EGFP which is set as 1. Some proteins are very dim (e.g. TagRFP657,...

...discovered in 1856 by William Perkin. His discovery set off a flurry of activity and the majority of the ...

.... suggest that it could be valuable in medical settings where antibiotic resistant bacteria have previously...Even when researchers develop new antibiotics, the onset of resistance is swift, as few as five years after...

...This modular approach combines an interchangeable set of fusogenic and targeting components to reprogram...Every few months, we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

...it’s simple. The RT protein is made by a separate set of instructions in the same producer cells as the...

...sales-y”. The work includes reviewing customer data sets, troubleshooting protocols, analyzing information...

... the use of a given timer in many experimental settings. Monomeric fluorescent protein timers Since the... simplifies cloning and makes your experimental setup less cumbersome. 3. Portability across multiple ...

...nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

...have become a powerful tool to address a diverse set of biological inquiries. References Ogura, Teru...death. The team used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed...for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be ... selects for those cells that have removed the cassette through a second recombination event between the...

...Either SYBR green technology or a TaqMan primer/probe set can be used and samples are quantified by comparing...

...difficult to use in a multicolor/multichannel imaging set-up. In contrast, smFP tags can be recognized by distinct...

... used successfully in preclinical and clinical settings for ocular conditions. However, AdV was abandoned...responsible for an autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...