We narrowed to 457 results for: val
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TypeBlog PostPublishedDec. 20, 2018, 1:15 p.m....controversy. Briefly, there are two overlapping, valid critiques being offered of He’s work: CRISPR/Cas9...
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Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog PostPublishedNov. 20, 2018, 8:17 p.m...., description, how the plasmid was generated, validated sequence information, and more. The plasmid page... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....to see what happens, but there isn’t really an equivalent of a “chemistry kit” for topics in biology, especially... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog PostPublishedOct. 26, 2017, 1:59 p.m.... Topic Page Learn How We Use NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....turned off quickly and with high precision - a valuable optogenetics feature when studying neuronal activity... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog PostPublishedJuly 21, 2020, 1:15 p.m.... DNA concentration. Generally, A260 of 1.0 is equivalent to 50 ug/ml pure dsDNA. Use the following formula... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....these widely studied signalling pathways. Find Validated gRNAs to use with your Cas9 Virus Pooled libraries... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....al. verified that translation is necessary for removal of GFP from the TRICK reporter. Delayed translation... -
Science Career Options
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m....Patience, accuracy and attention to detail are valuable skills acquired from a science degree that can... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....tuned” with mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....nucleofection as well as the sequences of our validated editing reagents can be found on our plasmid pages... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....biophysics from UCSF in the laboratory of Ronald Vale, after which he was a fellow at the Bauer Center... -
Advice for Scientists Starting a Lab
TypeBlog PostPublishedApril 29, 2014, 1:43 p.m....getting everyone talking to each other cannot be undervalued.” Connie Cepko points out the limited choices... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....shifts in expression, using a histogram plot and MFI values to characterize your samples may allow for better... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....easily sort them by other parameters, such as E-value for protein sequence searches. Additionally, the... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....provide resources, knowledge, and most importantly, validated protocols (using readily available reagents!) ... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....evolutionarily optimized gene delivery devices. Their survival depends on their ability to deliver and permanently... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....interpretation of results. Take extra care when evaluating how this serotype works in your system. Chemogenetic... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....for analyzing time-lapse images, quantitatively evaluating antibody staining, or counting cells are now ... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites and activity (Knudsen et al...