We narrowed to 783 results for: INA

...nature can inform us on how to create better recombinant vectors. In this blog post, we’ll define plasmid...harness those features to create broad host range recombinant vectors.  A plasmid’s host range is determined...phylogenetic groups (Jain and Srivastava 2013).  Recombinant vectors that are made from broad host range plasmids...host-independent   Building broad host range recombinant vectors Replicons, which consist of a plasmid...broad host range plasmids can be used to create recombinant vectors that can replicate in different bacteria...to incorporating broad host range replicons, recombinant vectors can be modified further to increase the...cloning sites (Keen 1988).  Find broad host range recombinant vectors at Addgene!   References and resources...

...not. The lack of an exemption for postdocs in the final ruling was, for a lot of us, frankly quite surprising...which leads to many complications, including the inability of some institutions to know exactly how many ...of 10.4%. The Icahn School of Medicine at Mount Sinai in New York is raising the minimum from $43,692 ... funding climate combined with the lack of a sustainable research enterprise, there is no easy way for...cheap trainee workforce has clearly not been a sustainable approach to biomedical research in the U.S. and...we must make real changes to make the system sustainable. For years, there have been recommendations that... they are necessary to produce a healthy and sustainable research ecosystem and that we cannot ignore ...

...guests, Melina Fan, Kenneth Fan, and Benjie Chen! Melina Fan is a co-founder of Addgene. Melina received... podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and Benjie Chen. We learn what it...University, where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge, ... led business efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to ...

...markers and homologous recombination As an alternative, endogenous homologous recombination enzymes can also...enzymes and those that use endogenous homologous recombination. Modifying bacterial genomes using exogenous...exchange requires two sequential homologous recombination events, but because these events are rare, each... ability to grow on sucrose, perhaps by sacB inactivation.  Bacterial toxins for counter-selection In ...mix. Introduction of the strong transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to ...genetic engineering in bacteria using homologous recombination." Current protocols in molecular biology 106.1...

... requires an N-terminal consensus sequence (MG-) and prenylation requires a C-terminal consensus sequence... filter sets on a fluorescence microscope, and finally (iv) good toleration in fusion proteins. Since ... introducing two mutations (F64L, S65T) in the original jellyfish GFP (AvGFP) to create enhanced GFP (... loop-structure (Querido and Chartrand, 2008). Finally, a nuclease-deficient Cas9 can bind loci in the...GFP is fused to these sequences. When N- and C-terminal fusions are not tolerated the alternative is to... subunit fusions that do not tolerate N- and C-terminal fusions (Adjobo-Hermans, 2011). Structural information...

...focus on defining these resources and not on the financial aspects. Money often carries an emotional weight...administrative services, managers, and techs’ financial books.  To find personnel salary ranges, go to...using animals, then that’s a cost that you can eliminate from your budget. Every cut you make signifies...signifies that you've made a conscious effort to eliminate some of the excessive costs in your lab. That in itself...specific aims. 2. Then make decisions based on financial values. All too often, we see early investigators...save you money in the short term. However, the financial risks associated with old equipment, like service.... Always focus on the science When negotiating finances, instead of solely discussing numerical values...

...single-stranded to double-stranded, and finally, drop once more during a final temperature increase. If there ...are cases in which it is an appropriate choice (Panina, 2018). Look for studies specifically aimed at ...real-time RT-PCR. J. Immunol. Methods 283:291–306.Panina, Y., Germond, A., Masui, S. et al. Validation of...M; Schwarz G; Pfaffl MW. 2003. Standardized determination of real-time PCR efficiency from a single reaction...Nucleic Acids Res. 31:e122.Thelwell N; Millington S; Solinas A; Booth J; Brown T. Mode of action and application..., Dong X, Zhang M, Hu G, Fang F. Fudenine, a C-terminal truncated rat homologue of mouse prominin, is ...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...

...Executive Director, Joanne Kamens, and founders, Melina Fan, Ken Fan, and Benjie Chen. Last week, the Addgene...weren’t able to join us in person. Joanne Kamens, Melina Fan, and Ken Fan each spoke about what Addgene ...mentioned Addgene’s incredible growth and our determination to keep materials as affordable and accessible...without whom Addgene could not do what it does.  Melina talked about her grandfather’s humble beginnings... heartfelt portion of the entire night was when Melina and Ken called their parents up to the podium to... great (maybe too great!) time. Ten years ago, Melina Fan had an idea and her family helped bring it ...

...genetic tools: activators, inhibitors, sensors, recombinases, and more. With the help of adeno-associated...continuously refined and developed so there are many combinations to choose from for your experiments! Late last... aimed at brainstorming strategies for using combinations of AAV-based molecular tools for targeted neuronal...specific expression of molecular tools and sensors Combinations of classical genetic tools, for example Cre/...restricted light exposure to a specific area in combination with delivery of AAV at the injection site results...specificity of the targeted cell population.  Combinatorial strategies can also be used for retrograde tracing...

...these devastating afflictions. Elia Di Schiavi - Spinal muscular atrophy Dr. Elia Di Schiavi is a Research...Bioresources in Naples, Italy. His group works on spinal muscular atrophy, a disease in which disruption...of the smn1 gene results in the degeneration of spinal cord motor neurons. This disease is estimated to...While disruption of the smn1 gene is known to cause spinal muscular atrophy, the molecular mechanisms behind...clinic and thereby restore motor neuron function in spinal muscular atrophy patients. Noushin Nabavi - Peritoneal...Systematic review of incidence and prevalence of spinal muscular atrophy (SMA)." European Journal of Paediatric...