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We narrowed to 821 results for: LAS

Showing: 161 - 170 of 821 results
  1. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...podríamos estar más emocionados! Después de recibir la traducción, Addgene contrató a una traductora científica...
  2. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable repression and activation of ....org/10.1038/nmeth.2600 Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) .../10.1038/s41598-019-54179-x Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD (2014) A Protein-Tagging..., Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE...Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your research ...
  3. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...by an optimized CRISPR/Cas system. Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park ...expression and fluorescence imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 23;159....1420024112. Epub 2015 Feb 23. Pubmed. Find the plasmids from this publication at Addgene. Dynamic imaging...10.1016/j.cell.2013.12.001. Pubmed. Find the plasmids from this publication at Addgene. A protein-....2014.09.039. Epub 2014 Oct 9. Pubmed. Find the plasmids from this publication at Addgene. ...
  4. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...programs. I spent one day at Addgene last summer, through the La Vida Scholars summer program. I was unsure... Amy was, which was the impression I got from the La Vida Scholars tour. After some time had passed, I...something wrong. My capstone project was based on plasmid cloning and I, the researcher, got to choose what... we found with the correct bands was the right plasmid, so after discussion, we sent it for sequencing...
  5. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...RNA-binding exonuclease protection factor La. They found that La, which is ubiquitously expressed in eukaryotes...tail of pegRNAs (Yan et al., 2024). The team fused La to the C-terminal end of PEmax to produce PE7. PE7... ~ ~ ~ ~ ✖ Fuse RNA-binding protein La to the prime editor Improve RNA stability   ...this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally...the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection ...for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration...
  6. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...Goldberg GW, Duportet X, Fischetti VA, Marraffini LA (2014) Exploiting CRISPR-Cas nucleases to produce...Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable repression and activation of ...04901-6 Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA (2013) RNA-guided editing of bacterial genomes using....1038/s41564-018-0327-z Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) .... citrea scarless editing plasmids Sheng Yang’s lab describes a two-plasmid system that combines recombineering...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...engineering functions in bacteria, but have no fear - plasmids from Addgene depositors are making it easier than...
  7. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...This editor includes the fused RNA-binding protein La to protect the end of the pegRNA. Adding 3′ polyU... epegRNAs) can improve either endogenous or fused La binding (Yan et al., 2024). Figure 9: epegRNAs...the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection ...
  8. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...–567 . https://doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead...doi.org/10.1038/nchembio.1753 Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD (2014) A Protein-Tagging...Last updated Oct 7, 2020 by Gabrielle Clouse. This post was contributed by guest bloggers Marcelle Tuttle...Inspired Engineering. CRISPR/Cas9 is an enormously plastic tool and has taken the scientific world by storm...et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences from a given...
  9. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...work is held by The Museum of Jurassic Technology in LA, where it is currently on display.   Many universities...Competition - a 40 year old international competition that last year received over 2,000 entries. Do you mix your... Source Lab Resources on Addgene.org Try the Plasmid Land Game ...
  10. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...then a postdoc at The Scripps Research Institute in La Jolla, California. She joined Elsevier after her ...everyone in your lab has been referencing for the last 10 years, and everything will be fine. It won’t....
Showing: 161 - 170 of 821 results