We narrowed to 884 results for: ica

...covered in detail here. Classical double-strand break repair (DSBR) In the classical DSBR pathway, the 3’...not be immediately applicable in DSB-induced genome engineering, it has biological importance for the ... PubMed. A Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771...pathways of DSBs, with a focus on repair for genome modification purposes. How does homology directed repair...and Homology Directed Repair (HDR). NHEJ is the canonical homology-independent pathway as it involves the... provided the DNA template used for repair is identical to the original DNA sequence at the DSB, or it...specific mutations into the damaged DNA if these modifications exist in the template DNA. There are several...

...calcium indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs...characteristics, performance, and applications of near-infrared calcium indicators in this recent review (Shcherbakova...expertise is development and applications of fluorescent proteins, genetically encoded biosensors, and optogenetic...Qian, Y. et al. A genetically encoded near-infrared fluorescent calcium ion indicator. Nat Methods 16, ...Shemetov, A.A. et al. A near-infrared genetically encoded calcium indicator for in vivo imaging. Nat Biotechnol...Shcherbakova, D.M. Near-infrared and far-red genetically encoded indicators of neuronal activity. J Neurosci Methods...information on NIR FPs, their properties and applications. At the end, there is a Table that summarizes...

...holding a physical model of the protein in your hand – you will understand the power of a physical model.... researchers use physical models of protein structure? We have been creating physical models of proteins... your hand? At 3D Molecular Designs, we create physical models of proteins using 3D printing technology...and I encourage you to hold one. Why do we need physical models of protein structure? Structural biologists...biologists have always struggled to effectively communicate the nuances of protein structure in a flat 2-D...problem with the development of Byron’s Bender – a mechanical device that could be used to bend 1/8th inch ... example, when Tom Steitz was presented with a physical model of his large ribosomal subunit, he kept ...

...like team building, collaborative critical thinking, and communication among others. They additionally ...therapeutic and pre-clinical problems! Who knows, perhaps the ideas spurred by this “gamification” will lead to...to develop strategies to make our classes more practical and hands-on. Students in these courses get plenty...playing a card game called “Magic”. There were complicated rules that governed how this game was played....upon who your challenger was, you might have to drastically alter your strategy. I realized that similar ... as I try being compelling, interactive, and application-driven to boost curiosity, I have never experienced...engineering and strategic knowledge to solve a clinical disease case. As a teacher, you explain the game...

...presented chronologically. With a resume however, most often people choose a reversed chronological style ... job asks for experience in “statistical analysis”, mention “statistical analysis” (word by word) in your...together a grant application? Are you familiar with the publishing or patent application processes? How ... pages in a Curricula Vitae to showcase your publications, academic performances, and research experiences... applying for. Also remember that a resume is typically no more than 2 pages long. Write your resume for...information. Think "skills," not just "research and publications” What are the skills you developed through your...handled large data sets and are familiar with statistical analysis. If you have done fluorescent imaging...

...circle. Typically, a yellow check mark indicates that a particular parameter was automatically adjusted... The vertical black dotted line represents the cut site. Cutting and error-prone repair typically result...content. Many thanks to our guest blogger, Jessica Roginsky. Jessica Roginsky leads the scientific support ...This article was contributed by Jessica Roginsky, Scientific Support Lead at Synthego. Article source... of robust software tools. As a consequence, a critical step in the gene editing workflow - analyzing ...either one at a time or as a batch of hundreds, and indicate the guide RNA sequence you used. The ICE tool ...and interpret data about these types of edits is critical to the CRISPR workflow. For a multiplex sample...

...preference.   Historically, ICSS was done by implanting an electrode and non-specifically electrically stimulating...the steps and technical considerations are directly applicable to the rat [6]. Technical differences include...A high-light sensitivity optical neural silencer: development and application to optogenetic control of...[2]. Laser activation is usually controlled automatically using motion capture software such as EthoVision...EthoVision. Consult the literature to find all the applications of optogenetics relevant to your field. Ultimately..., regardless of the type of behavior, certain technical considerations still apply. Testing stimulation...Inhibition of neuronal activation is a little more complicated since basal neuronal activity in your brain region...

...detection is crucial for clinical diagnostics, genotyping, and biotechnological advancements. Many methods...method can be used for an array of applications including clinical diagnostics (e.g. pathogen or virus...population for an array of molecular and clinical diagnostic applications. The company Mammoth Biosciences has...activated” Cas13 produces a quantifiable signal that indicates the presence of your targeted nucleic acid. To...amplified using RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-RPA, respectively...RNA for subsequent detection by Cas13. This amplification step in combination with the ssRNA reporter ... introduced in 2017 so the lab made further modifications to improve their detection system. They named...

...intuitive, or logical to a biologist that are not at all obvious, intuitive, or logical to a layperson...from other sources. Mode of communication Different modes of communication take up different amounts of...your audience needs to understand what you’re communicating. While I’m sure that there are loads of ways...new languages!) Jargon has a place in science communication, but the more you use, the higher cognitive ...This can be obvious, in that a lot of science communication is helping people understand new concepts. You...layperson. When you’re communicating ideas that you think are clearly connected, step back and ask yourself, ...pick and choose what concepts and details to communicate. It’s okay (and encouraged) to leave blank spaces...

...ability to specifically recognize and bind proteins. They’re used in a number of research and clinical applications... antibody’s ‘Y’. They are often used in clinical applications. Fab fragments can be created in lab in ...Shahied, 2004). They are often used for clinical applications, including imaging.  Diabodies are ~60 kDa...deeper penetration into a tissue, useful for applications like IHC, or be able to access an epitope folded...that would size-exclude an antibody. In imaging applications, smaller affinity reagents means higher resolution...Designed ankyrin repeat proteins, or DARPins, are genetically engineered small affinity proteins, around 14...proteins known as ankyrin repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity ...