We narrowed to 820 results for: APP

...Paul Blainey’s lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi...for microglia research and broader therapeutic applications.  Find microglia-targeting AAV plasmids here...reconstituting coding sequences for a range of applications, including CRISPR activation by dCas9-VPR, prime...

... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...chromatic-opening agents to boost expression to appreciable levels. We wanted to create second-generation...ultrasound imaging of nanoscale acoustic biomolecules. Appl. Phys. Lett. 110, 073704 (2017). Hurt, R. C. et ...

...institutions, or institutions they're thinking about applying to, are doing - for example, your benefits may...get rid of postdocs; that current postdoctoral appointments may shorten in length; that postdoc salaries...trainee workforce has clearly not been a sustainable approach to biomedical research in the U.S. and postdoctoral...

...especially with industry. Today we also have the appropriate tools to verify our cells lines. Even if you ...the results are reliable. With this two-pronged approach, the many mistakes of the past can be prevented...including GSK, AstraZeneca-MedImmune, and Infors & Applikon. Due to his high level of experience he now travels...

... explain how to verify that your cells were appropriately edited for your desired mutation - insertion...frequency is 50%, then you could infer that approximately 25% of cells are likely -/-, 50% are -/+, and...sequencing confirmation. All knock-ins A method applicable to knock-ins both large and small is a software...

...References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled...Scarless Modification of Proteobacterial Genomes." Applied and environmental microbiology (2019): AEM-00990...chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015): 5103-5114. PubMed...

...study its function is one of the most popular applications of fluorescent proteins. These fusion proteins...targeted to the endoplasmid reticulum based on the appearance of OSER whorls. This assay is quantitative and...linker sequence short In the early days of GFP applications, many were concerned with steric hindrance by...

...enjoy a warm meal on a winter day, you should be happy to learn that many DNA-based reagents are named ...aptamer soup by exploring the development and applications of fluorescent RNA aptamers in Addgene’s collection...fluorescence when bound to DFHBI despite being approximately half the length (Warner et al., 2014); its length...

... barrier of the endothelium, but they could be applied to study other actin-dependent processes in cells...) and to introduce unnatural head groups for applications like fluorescent labeling via click chemistry...constructs are great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks...

...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...can be engineered to bind specific RNA bases. By swapping out these different modular repeat segments, you...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature...

...popular choice for a variety of synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A....that they could reprogram the genomic target by swapping the homology arms for new targets. And, importantly... is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger...

...for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi...cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi...expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res...

...to specific DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety... publication at Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy...Ruan K, Wang F, Xu G, Hu R (2016) A CRISPR-based approach for targeted DNA demethylation. Cell Discovery...

...tools Once you’ve selected the class of tool appropriate for your experiment, you’ll need to find a specific...Yizhar, O., & Deisseroth, K. (2012). Principles for applying optogenetic tools derived from direct comparative...

...have been increasingly popular in fundamental and applied research since their first use in the early 90’... at transferring the gene of interest to the appropriate cells, it was obvious that its safety was a disadvantage...either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population of cells might express only the ...

...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...the coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, ...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring...

...cross-team guy” at Addgene, an unofficial title that I happily embrace (and admittedly, I may have started that...identify as one.  But some time around 10th grade, I snapped out of the phase of my life that had me listening...you’re part of a team - it will just make you a happier person. What does a good team need? Besides that...

...huge innovation, there was a limitation to this approach. CBEs and ABEs are base transition editors. They...GBEs), Xueli Zhang’s lab, returned with a unique approach to improving C → G editing. Instead of fusing ...abasic site. In this post, it refers to the same approach as a CGBE. BER Base Excision Repair A cellular...

...chance that all the edits you want to make will happen.  There are many reasons to multiplex gRNAs, some...adjacent to the sites. When digested with the appropriate Type IIS enzyme, the unique flanking overhang...subsequent “bricks” to be added one by one. This approach is especially useful for combinatorial analyses...

...process is favored over the other. Cell conversion happens via: ReprogrammingA differentiated somatic cell...cells into another cell type. However, these two approaches differ in several ways. Below is a table listing...: 26042384. 27. Nejadnik, H., et al., Improved approach for chondrogenic differentiation of human induced...

...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

...wintertime festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the... the New Year. Will your lab be a lucky winner? Happy holidays from all of us at Addgene! We look forward...

... Why #DecktheLab? Help us spread some cheer by snapping a photo of you and your labmates celebrating the... the New Year. Will your lab be a lucky winner? Happy holidays from all of us at Addgene! We look forward...

... when Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast...Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about SITN's work at DayCon ...

...Performing In Vivo CRISPR Screens Using the FITS Approach Caitlin Faulds, who introduced the very first...Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and...

... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...

...reveal the winning entries on Twitter and Instagram! Happy decorating, all, and to all a good light!    🤩 ...set a challenge to my lab mates. They did not disappoint. #deckthelab pic.twitter.com/X4eKUGnQMe— Alex...

... Molecular biologist with Biogen interested in applying CRISPR for mouse genome editing. Post: Generating...Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein College of...

... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

...an organism’s genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...