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We narrowed to 807 results for: APP

Showing: 481 - 510 of 807 results

  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...popular choice for a variety of synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A....that they could reprogram the genomic target by swapping the homology arms for new targets. And, importantly... is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger...

  2. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...tools Once you’ve selected the class of tool appropriate for your experiment, you’ll need to find a specific...Yizhar, O., & Deisseroth, K. (2012). Principles for applying optogenetic tools derived from direct comparative...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi...cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi...expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res...

  4. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...the coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, ...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring...

  5. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...to specific DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety... publication at Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy...Ruan K, Wang F, Xu G, Hu R (2016) A CRISPR-based approach for targeted DNA demethylation. Cell Discovery...

  6. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...have been increasingly popular in fundamental and applied research since their first use in the early 90’... at transferring the gene of interest to the appropriate cells, it was obvious that its safety was a disadvantage...either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population of cells might express only the ...

  7. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...cross-team guy” at Addgene, an unofficial title that I happily embrace (and admittedly, I may have started that...identify as one.  But some time around 10th grade, I snapped out of the phase of my life that had me listening...you’re part of a team - it will just make you a happier person. What does a good team need? Besides that...

  8. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...huge innovation, there was a limitation to this approach. CBEs and ABEs are base transition editors. They...GBEs), Xueli Zhang’s lab, returned with a unique approach to improving C → G editing. Instead of fusing ...abasic site. In this post, it refers to the same approach as a CGBE. BER Base Excision Repair A cellular...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...chance that all the edits you want to make will happen.  There are many reasons to multiplex gRNAs, some...adjacent to the sites. When digested with the appropriate Type IIS enzyme, the unique flanking overhang...subsequent “bricks” to be added one by one. This approach is especially useful for combinatorial analyses...

  11. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

  12. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    Published
    Nov. 30, 2015, 3:30 p.m.
    ...wintertime festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the... the New Year. Will your lab be a lucky winner? Happy holidays from all of us at Addgene! We look forward...

  13. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ... Why #DecktheLab? Help us spread some cheer by snapping a photo of you and your labmates celebrating the... the New Year. Will your lab be a lucky winner? Happy holidays from all of us at Addgene! We look forward...

  14. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ... when Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast...Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about SITN's work at DayCon ...

  15. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...Performing In Vivo CRISPR Screens Using the FITS Approach Caitlin Faulds, who introduced the very first...Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and...

  16. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...

  17. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...reveal the winning entries on Twitter and Instagram! Happy decorating, all, and to all a good light!    🤩 ...set a challenge to my lab mates. They did not disappoint. #deckthelab pic.twitter.com/X4eKUGnQMe— Alex...

  18. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... Molecular biologist with Biogen interested in applying CRISPR for mouse genome editing. Post: Generating...Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein College of...

  21. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...an organism’s genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...

  22. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

  23. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ... in 100 µL aliquots, so the volume options will appear as 10, 25, or 50 units.  Once your request is received... you find this service useful in your research. Happy vectoring! ...

  24. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ...awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...scientist's molecular biology needs and why Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:...

  25. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ... certain mystique that makes them difficult to approach for the youngster just starting in the lab, but... a graduate student? 4:50 - 8:00 : How did you approach doing rotations? 8:01 - 11:25 : How did you decide...

  27. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...when budgets are constrained. This collaborative approach eliminates redundant work, saving time and allowing...be listed as direct costs from a CRO in grant applications, addressing the new funding structures and indirect...

  28. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...night, so why leave your lab equipment on? This applies to water baths, computers, and more. Consolidate...to the Addgene blog Learn about other creative approaches for lab sustainability Resources on Addgene....

  29. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ... shipped! That's a 1/2 million plasmids sent to happy scientists around the world in ~10 years! We're ... as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team recently...

  30. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...talks, symposiums, poster sessions, exhibit booths, happy hour socials, and late, late night socials. As a...depositing scientists at #SfN14. And we've been so happy to hear that we've been able to help their labs ...
Showing: 481 - 510 of 807 results