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We narrowed to 714 results for: MAK

Showing: 481 - 510 of 714 results
  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...exquisite for the so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees... modification of interest, it would be unwise to make conclusions on the basis of the activity of a single...
  2. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...immunoglobulin (Ig) heavy chains and two Ig light chains make up each antibody molecule. The Fc region and part...epitopes on the protein. However, each animal will make antibodies against different protein epitopes and...
  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...during chemical synthesis of these molecules. To make large amounts of pure product in an affordable way...constitutive and inducible promoters, linkers, and tags makes EcoFlex a great option for those interested in recombinant...
  4. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...switch from a dark state to a red fluorescent state make them useful for 2-color imaging with green PA-FPs...fluorescence usually means more photons are being emitted, making it easier to capture a clear image above background...
  5. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...controls needed to pressure-test your FRET assay to make sure it is working? This article addresses these...FRET experiments. However, remember that, if you make an FP fusion, you are inserting about 20-25 kDa ...
  6. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... plateau stage after exponential amplification, making it difficult to properly analyze your data.  To...understanding how the analyses work - you do need to make sure the assumptions in your dataset match the assumptions...
  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...The ease and flexibility of the LACE technology makes this system widely accessible for many potential...interest within the INT location. One plasmid to make note of is the INT construct bearing the "Bunch ...previous studies, and provide new insights into the make-up of multiprotein complexes like the EPC1 complex...
  8. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ... While helpful from a biological standpoint, it makes delivering AAVs to the brain difficult. Traditionally... References Asencor, A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & Chaudhari, N. (2022). Selectively...
  9. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...vectors for many experimental purposes. To help you make sense of all the viral vector information that's...
  10. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...section and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...
  11. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...names as finalists. Now, we’re asking you to help us make the decision between some meaningful names…. Watch...
  12. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...pipette boxes, wire, cable ties) received a festive makeover today✂️❄️🦠thanks to the awesome templates by ...
  13. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...being used for bacterial genome engineering. To make it easier for you to find plasmids for your work...
  14. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...environmentally responsible manner.  Other ways to make the office more eco-friendly The switch to 100% ...
  15. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...suggestions here or by using the hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we...
  16. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...Editors: Modified versions of CRISPR nucleases that make single base changes in the DNA sequence without ...
  17. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...CRISPR X from Michael Bassik's Lab - A new tool for making diverse, localized point mutations. Listen to Our...
  18. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...news (pop culture events of the past year obviously make for good costumes), science and our plasmid collection...
Showing: 481 - 510 of 714 results