We narrowed to 868 results for: REN

...light-induced cell ablation with MG-2I-dL5** in two different experimental settings: HEK293 cell culture and...these experiments because developing embryos are transparent, so it’s easy to watch development in real time...using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato, M. A., Onuoha...Chromophore-assisted laser inactivation in cell biology. Trends in Cell Biology,18(9), 443-450. doi:10.1016/j.tcb...

...principal investigators. They also come from many different scientific fields, from social sciences to biology.... We now want to use this potential to make a difference not just against COVID-19, but also in other ...scientist, and proud member of Crowdfight. He is currently based in Spain and works on climate change. You...one of the coordinators at Crowdfight. She is currently based in Spain and works in the field of genetics...

...identifiers (PIDs), a long-lasting way to identify and reference documents, files, or physical reagents. What ...you, we’ll go through examples to highlight the difference between them using a plasmid available through...the lenti-Cas9-VQR plasmid page. Despite their differences, both of these PID formats for lenti-Cas9-VQR...managed by Addgene. How to cite options using the different PID formats The instructions for how to cite any...

... that affect Th2 differentiation and activation, using expression of differentiation factors as the screen...they develop rapidly, they are small, and their transparency allows for whole organism imaging. They created...Gottschalk group hypothesizes that this may be due to different lipid compositions of C. elegans membranes, which...

...personal assistant has to deal with. My inbox currently stands at about 65,000 messages and dealing with... lab and safety manager, researcher, adviser, conference planner, and writer.” Connie Cepko was surprised...every graduate student or postdoc may require different types of mentoring. Some mentees prefer lots of...impossible' or useless.” How do you run your lab differently or the same as your adviser did? Tom Ellis merges...

...Which is why, at the Society for Neuroscience conference in November 2023, we hosted our very first professional... who is my audience, and what do I want to be different for them when I’m done? What are we trying to ...    Here, this image is being used to convey the difference between a gradient tube before and after ultracentrifugation... For instance, you would present information differently in a poster compared to an online abstract or...

...high and your immunogenicity low! References and Resources References Robinson HL. Retroviruses and cancer...infect humans and some other mammals but is not currently known to cause any diseases. Recombinant AAV largely...excellent features for human gene therapy and is currently being used in clinical settings. AAV is also ...

...next few years, with the goal of creating more transparent and reproducible science in the long run (Hohlbein...problem-solving platforms.     Fig. 3: Different workflows involved in sharing open hardware. Upper...microscope. Lower left panel shows an image of differentiating monocyte with planes and hardware to it. Right...image processing and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling...

..., without the FMO, it would be difficult to differentiate the Ly6C negative population from the Ly6C positive...like for a particular marker, you can use the reference plots provided by the antibody companies or check... use the APC IgG2c isotype control). The only difference is that the isotype antibody will not target ...CD8+ T cells and cancer immunotherapies. She is currently a research technician in Arlene Sharpe's lab at...

...variety of educational programs all focused on current questions and experiments in the field of synthetic...adapted from her own MIT curriculum. Drawing on current questions being asked in MIT’s research labs, BioBuilder...exploration, discovery, and collaboration. One module currently under development comes from the Caltech lab of...students include The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically...

...mission. In no situation is this more true than the current global pandemic in which scientists race to find...from Ginkgo Bioworks are available for request? Currently, plasmids containing all SARS-CoV-2 protein coding...requested the plasmid. By leveraging our core strengths of intensive screening and the use of high throughput...Visit our COVID-19 Plasmids and Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped...

...without a DNA double-strand break. But base editors aren’t perfect. They may be slow, can only target certain...culture). This dilution step represents the major difference between PACE and PANCE: for PANCE the phage progeny...editor activity and editing speed. Created with BioRender.com. Introducing ABE8e - a fast and flexible ...much more efficiently and in more contexts. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran...

... biosensor - IISER Bhopal is back in the SynBio arena this year with a fresh team of twenty excited undergrads...extremophiles to survive under these conditions are currently unknown, and it’ll undoubtedly be quite a while...to guess. Think about it like winter tires in different conditions: the more flexible rubber of winter...spirit of scientific innovation amongst school children through summer workshops and interactive discussions...

...experiment moving. Happy editing! References and Resources References Hendel, A., Bak, R., Clark, J. et...talk about their precious RNA samples. RNA is inherently less stable than most macromolecules used in ...region of the gRNA. These gRNAs can be cleaved and rendered useless after less than a minute of exposure to...

...AAV8) enable different patterns of transduction and transgene expression across different cell types (.... Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses...visualizing hM4D(Gi)-mCherry in fixed tissue. References 1. Aschauer DF, Kreuz S, Rumpel S. Analysis of...

...widely utilized across many different cell types and each one requires different elements for vector propagation...Instead, the size and A-T content of the DNA (apparently independent of a known specific sequence) dictate...auxotrophic markers and includes the associated references.  Yeast selection marker What does the marker...

...formal mentoring program at my company, but there weren't enough senior people willing to step up and be ...hundreds of mentoring groups through my work with the renowned Healthcare Businesswomen’s Association (HBA) Boston...commitment of a formal mentoring relationship or there aren’t enough senior mentors available to go around. My...these criteria. If you can, include people from different labs, departments or organizations as it brings...

...stem cells edited to carry mutant versions of 15 different disease-associated genes. Addgene spoke to Musunuru...mutations. Once that's done in stem cells, we can differentiate those mutant and wild type stem cells into any...all have the mutations but they will also have different genetic backgrounds. It becomes more of an apples-to-oranges...suggest TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN genome-editing system...

...sixty seconds of silence–but it was thorough and coherent.  To be honest, if my paper hadn’t been ridiculously...to stop talking until I could present something coherent or to quickly string together all the words that...screeching feedback. You’re muted on Zoom. Your video conferencing software freezes and then crashes. You’re muted... a clear, informative presentation, speak at conferences, or even just provide quick updates during a ...

...if you need a very pure sample. Because binding strength is proportional to the length of the tag, with...Antibodies.) Unfortunately, for some proteins there just aren’t any good antibodies available. In these cases, ...antibody posts from our blog!  Resources and References  Resources on addgene.org Addgene Antibody Guide...ChIP Antibodies 101: Introduction to Antibodies References Hochuli E, Bannwarth W, Döbeli H, Gentz R and...

...but where the kinases are located, their signal strength, and how long their signals last all impact transduction... or “on.” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when on). Figure 1...function of the protein and pdDronpa are preserved. References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, ...28232577. Figures 2-4 were modified from this reference. 2. Regot, Sergi, Jacob J. Hughey, Bryce T. Bajar...

...antibodies Conventional antibodies are a non-renewable reagent - Their production requires animal sacrifice...produce - Hybridomas offer an alternative and renewable source of monoclonal antibodies, but they are ... their use. Each RANbody is detectable by two different tags: a reporter and an HA tag. The reporter comes... link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-...

...SciComm by doing SciComm Writing had always been a strength of Susan’s, but she didn’t know if she liked science... of a team.” It took her two years to find her current position at Fred Hutch because her skill set wasn...connections she made through this program lead to her current job. “One of my colleagues in the class was like...Hutch magazine. Getting pulled in all of these different directions is sometimes at odds with the space...

...mammalian systems has been in question. Three different labs have recently published work that provides... gene activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best...miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1...of up to 10kb but it is also capable of coding concurrent small insertions to allow in-frame deletions ...

...extra care when performing RNA extraction. RNA is inherently not as stable as DNA- it is single stranded and...ultracentrifuge.  Figure 1: An outline of the different steps in RNA extraction. Researchers at the... Reagent®, RNAzol®, QIAzol®  and sold by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol... ensure DNA contamination is not an issue.  References                 Allewell NM, Sama A (1974) The...

... vector team is publishing research, hosting conferences, and are expanding our catalog of materials to...appearance and functionality are happening and this strengthens our image in the world. Addgene is now as much...practices and benefits and these are making a difference. We serve scientists around the globe and our...Thanks to all the Addgenies and congratulations to current and alumni Addgenies on our success. Thanks to ...

...the target strand and the gRNA. Created with BioRender.com. To decrease the electropositivity of the...mutations). Although the precise mutations were different than those found in eSpCas9, the effect on specificity...and/or therapeutic settings. Will combining the different mutations found in each of these great tools further...continued improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered...

...scientific manuscript will require numerous other references to scientific literature to substantiate the ...literature related to the topic. Finding reliable references and sorting out what they mean is no small task...focus in on a particular conclusion. Many papers aren’t always written this way and there is a greater ...inspiration and accountability. There are a few different ways to automate the process as well. Sign up ...

...without permanently altering the DNA, CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) are...study ncRNA, CRISPR systems offer a number of different options. By carefully considering your ncRNA of... you more about its function.  Resources and references Resources on addgene.org Addgene's CRISPR Guide... CRISPR enzymes for transcriptome engineering References Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna...

...CRISPR experiment. Happy cloning! References and Resources References McGee, A. V., Liu, Y. V., Griffith...chosen because of their broad applicability in different model systems. What’s the error rate? The Doench...Table 1 has a list of Fragmid destination vectors currently available. Table 1: Fragmid destination vectors...