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  2. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...line. When expressing a viral construct transiently care needs to be taken to ensure that the proper cell...

  4. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...the themes in the field and to learn what people cared about. I also used reviews to get a list of research...

  5. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... clones. The entry clone and destination vector carry different antibiotic resistance markers (indicated...

  9. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within...

  10. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...Figure 1: Addgene’s Agile Approach Sharing is caring There are many moving parts to our software, which...

  11. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...followed bylanding site recognition and integration of cargo via Cas9-directed integrases.   Use PASTE can...

  12. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted by the amount...

  14. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...Wang, Haoyi, et al. "One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated...

  17. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ... are many, many different types of experiments carried out by scientists every day. Although the designs...

  18. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...and cannot be used for dynamic measurements. Extra care should be taken so as not to destroy the donor molecule...

  22. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...fluorophores, more are in development, including Carrot and Radish from the lab that developed Corn. Aptamers...

  24. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ..., the rat is frequently used to study human cardiovascular disease, specifically stroke and hypertension...

  26. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...fluorescence signal intensity. However, you need to be careful when selecting the primary antibody host species...

  27. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ... robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA...
Showing: 481 - 510 of 523 results