We narrowed to 833 results for: Cat
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TypeBlog PostPublishedMarch 17, 2021, 2:40 p.m....where I foster dogs and help screen dog adoption applications. My most recent foster dog was with us for two... small claims court summary process. Julie is dedicated to volunteerism and ran the Mayor’s Midnight Sun...
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The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA ... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection... -
Reflections and Looking Ahead
TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m....accessible and reliable. We continue to expand our educational content supporting the tools in our collection... growing community on Bluesky, our expanding educational resources, and our commitment to global accessibility... -
Editor's Choice, September 2016
TypeBlog PostPublishedOct. 7, 2016, 4 p.m....Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific Enterprise in ... a Lab and a Project That’s it for now. As I indicated in the opening paragraph, I’m currently on my ... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog PostPublishedOct. 31, 2019, 1:06 p.m.... into a pulsating display imitating a moving caterpillar. The worm controls the snail’s behavior, forcing...exposed to predators. Hungry birds spots the “caterpillar” and then poke out the snail’s eyes and ingests... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog PostPublishedJan. 10, 2019, 2:38 p.m....researcher may only know how to prepare phage for one application but not another or they may have only used phage...Directory expands, it can also fill this role by cataloging and archiving information on individual phages... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog PostPublishedJuly 21, 2015, 2:30 p.m....determining meaningful solutions. PI-trainee communication builds a positive lab culture Although they ... communication... -
Science as Art
TypeBlog PostPublishedMarch 2, 2015, 3:13 p.m....problem is that scientists sometimes forget how to communicate about their work with non-academic people.” Daniel... new method to explore the inner structures of delicate plants and other organisms without destroying ... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog PostPublishedDec. 17, 2013, 2:47 p.m.... helps you reach your goals Listens well and communicates clearly and openly Gives honest feedback without...lab. If only every science trainee had such a dedicated person guiding the start of their career. “I want... -
What's New in CRISPR - March 2020
TypeBlog PostPublishedMarch 10, 2020, 1:15 p.m....expression of specific Caulobacter genes using a catalytically dead Cas9 (dCas9) derived from Streptococcus...methylation-silenced genes. Read the paper in Nature Communications Find Casilio-ME plasmids If you have a new... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog PostPublishedOct. 15, 2024, 1:15 p.m....request is approved, you will receive an email notification that your request is ready to order. An “Add...subscribe to the Addgene blog for updates and educational resources. We hope you’re as excited about this... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog PostPublishedJan. 23, 2024, 2:15 p.m.... validation data. We’ll continue growing our educational resources, with the recent release of the Spanish.... Through an expansion of our export license application service, we’re pleased to announce that we are... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m....something doesn’t work, it’s not a failure. It’s an education. Figure 4: Zenodo page showing the IBEX... repository for reagents, protocols, panels, publications, software, and datasets. It’s also a great place... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog PostPublishedDec. 5, 2019, 1:49 p.m....important. It needs to express value, clearly communicate exactly what a site offers to their users, and...hug items closer and do so more animatedly. To communicate that sense of community, Blugene will regularly... -
A Better Way to Get Customer Support: The Help Center
TypeBlog PostPublishedDec. 10, 2015, 3:30 p.m....right ones, quickly, and this is why we have a dedicated support team that can help you through any search...webpage, you can browse through our various FAQ categories or search through the entire FAQ collection. ... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....Gerald Rubin’s lab sought to make complex genome modifications in Drosophila using multiple recombinases. To...offspring carried both the recombinase and GAL4, indicating that the recombinases were inherited normally... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog PostPublishedJuly 29, 2014, 6:16 p.m....,300 plasmids along with image galleries and educational resources to go with them. This collection of...These plasmids are divided into the following categories: Over 300 empty backbones with 100+ distinct... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....interactions for subsequent sheering of DNA by sonification. Though it helps capture weaker or more transient...change offers a significant improvement and simplification of the original technique, specifically in ... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....tape the box shut, and drop off the box at any location where the United States Postal Service picks up... of the container, and drop off the box at any location where the United States Postal Service picks up... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m....Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the...biosensors Resources on Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog PostPublishedJune 29, 2023, 7 p.m....time for me to complete an online social media certification on social media data, and I was given a fantastic... at Northeastern and Addgene co-op studying communications, marketing, and global fashion studies. She... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....put into them in those later stages. Target identification Not all diseases are fully characterized or...simple pathology. In these cases, preliminary identification of potential targets by CRISPR can accelerate... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....problems, develop general technical proficiency, communicate your work, maintain ethical standards and interact...websites listing alumi and it is easy to use publication records, university websites, ResearchGate and... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based...and mature HA-tagged proteins in vivo. Nature Communications, 10(1). PubMed PMID: 31270320. PubMed Central... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m....be a time when you need to speak publicly to communicate your work. One of my 10 tips for giving a great...; Graduate Student Funding Opportunities Open Education Database: 100 Places to Find Funding For Your ... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....in @nchembio Title: Bye bye L-arabinose drive. Dedicated to Edo, Armin and @KhammashLab. I hope you all...reached the level of expression needed for most applications. Figure 1: With blue light, the VVD... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m.... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in...Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....published, knockout validated, and work in your application. But…they are the same isotype and therefore ...are identical for antibodies within certain classification groups called isotypes. Why does isotype matter... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....launching a company without partners. You need a dedicated team that you know well and trust. This will ...Chowdhury and Clinton Buell, who have very diverse educational backgrounds (an essential component to any successful... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m.... interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease...library of characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer Cruz-... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog PostPublishedMarch 6, 2014, 7:12 p.m....will you meet? How does the Mentor prefer to communicate between meetings (email, phone, etc.)? Make agendas...skills Support inclusion, diversity, and open communication Feel valued by giving back Final tips for success... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....brain, which has humanistic and philosophical implications. As an example, a group at Caltech used an optical...a handle on some of these mysteries. Another application is clinical; can we find ways to treat patients... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....generation when bound by the dL5** FAP. The iodine-modifications also shift MG-2I’s excitation wavelength to ...FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation with MG-2I-dL5... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...lines. In the meantime, be sure to browse the publication references and web links from my first post and... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....smaller fragments that are then barcoded for identification. This mixture of barcoded fragments is known...of Toronto. He is an author of 20+ scientific publications and patents. ... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....represent the fraction of that total with the indicated function. One huge reason CRISPR has become such...achieving the gRNA representation recommended in the publications associated with these libraries. Please note... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....sometimes we don't." - Jennifer Tsang, Science Communications and Marketing Coordinator Non-science favorites...times." - Aliyah Weinstein, Marketing and Communications Manager Addgene's book club Indeed, reading... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....plasmids at Addgene Opperman J et al., Nature Communications, 2019. PubMed PMID: 31346176. Using LADL to...light-activated-dynamic-looping (LADL) system. The group fused dCas9 to a truncated CIB1 protein from Arabidopsis (CIBN), which they... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog PostPublishedApril 28, 2021, 1:15 p.m....research papers or dissertations. Phrases are categorized either based on the section of a manuscript they...introduction, methods, results; or phrases are categorized by the goal of the phrase, such as being cautious... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar agreements...portal automates a lot of the process, from notifications and emails to generating the UBMTA link for ...