We narrowed to 801 results for: DES

...the Streak Plate Challenge, Westenberg’s course includes Winogradsky columns and characterizing the microbes...

...Plasmids 101 blog series Learn about the different grades of DNA  Read about the various ways to quantify...

...community and support a wide range of experimental designs. Here are nine great additions, including our first...

...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics Adesnik New viral vector pAAV-nEF-Con/Foff DREADD Gi-...

...Breakdown (Time : Topic) 0:00 - 0:28 : Niroshi and Ben describe their projects 0:28 - 1:12 : Introduction from...

... which results in neutralization or targeted destruction.  Antibodies are composed of two light chains...

...Addgene's Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench...

...travels from 2015, including a great European photo slideshow from our UK team member Benoit Giquel. Bluegene...

...information in our lab software, labeling stabs with barcodes as they go. Stabs are organized by shipment and...

...that the most cited work in history is a 1951 description of an assay to determine the amount of protein...

...other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...

...,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs targeting intergenic regions. The...

...our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral preps! Download...

...job of providing skills training for scientists destined to continue in academia. It seems it’s largely...

...work with any group of trainees interested in designing career exploration programs or peer mentoring ...

...are usually unresolved and small, so I suggest designing your primer at least 50bp upstream of the sequence...

...press release. "In the future, it could allow us to design versions of these editing tools that are more specific...

...University Center for Regenerative Medicine. Darrell describes his career goals and what drives him as a praticing...

...databases, DNA sequence manipulation, and primer design. Best wishes for the new year and your research...

...think is the most important resource that Addgene provides the scientific community? Access to plasmids which...

... who started the BALSA group, a nonprofit that provides short-term consulting services. Initiative and...

...University Technology Managers (AUTM). This MTA provides many benefits for both the providing and receiving...

...only be expressed in neurons. Indeed, this AAV provides you with a means to specifically activate expression...

... brand friendly. I introduced the team to a new design program, Canva, and I was able to create cute and...

... blog posts on prime editing, IgnaviCas9, and Nanoblades), but we hope that this blog series will help...

...communication between all members of a team. As described here in a 2012 Intuit blog post, a study coming...

...     Science’s John Bohannon published an article describing a sting operation in which he submitted a phony...

... that 2014 will be a year of lab BBQ parties - despite BBQs being wishful thinking in England. The Cepko...

...rare diseases. These underrepresented individuals deserve a voice. Check back with Addgene often to learn...

...internationally COVID-19 Resources Canada is a database that provides information about reagents, funding and volunteer...

... whose expression varies depending on transgene design and delivery. They generated hPSC lines with AAVS1...

...accessibility updates include a printer-friendly design and alt text on all images. We have also made accessibility...

...will need to be accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG...

...PAM sequences required by each ortholog, a gRNA designed for one dCas9 should be specific to that ortholog...

...multiple recombinases can be used together to designate specific patterns of recombination. Finally, Rubin...

...He charged the postdocs to “Grab ahold of your destinies” and also suggested some concrete actions that...

...with genes knocked out in the insulin signaling cascades in only 4 months compared to a year or greater...

...already made for them,” Baird said. “They are all designed in the same vector backbone with very similar ...

...deposited at Addgene by over 250 scientists in Europe. Despite these fantastic numbers, a lot of European scientists...

...entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped away from...