960 results

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.

Rationally engineered Cas9 variants eSpCas9 SpCas9-HF1 enhance CRISPR on-target specificity. Learn how in this post.

Dec. 16, 2015, 3:30 p.m.

We've made it easier than ever to get support for our resources - Check out the new help center for answers to all of your frequently asked questions.

Dec. 10, 2015, 3:30 p.m.

Addgene Board Member Connie Cepko gives us the scoop on progress in retinal gene therapies and the development of GFP-activatable tools in her lab.

Dec. 8, 2015, 3:30 p.m.

The Addgene blog has expanded tremendously in 2015. Check out our curated list of our top viewed posts from 2015!

Dec. 7, 2015, 6 p.m.

Joanne Kamens provides highlights from the 2015 Canton Nucleic Acids Forum (CNAF).

Dec. 1, 2015, 3:30 p.m.

Win a tasty winter treat in Addgene's #DeckTheLab holiday cheer lab photo contest. See details inside.

Nov. 30, 2015, 3:30 p.m.

Read our highlights from the Future of Research Symposium Boston 2015 and how scientists are tackling structural problems in academic research.

Nov. 25, 2015, 4 p.m.

Connie Cepko's lab at Harvard University has developed transcription factor and CRE recombinase variants activated by GFP. Learn more inside!

Nov. 24, 2015, 3:30 p.m.

In this blog post, learn about a new FRET-based biosensor for studying epigenetic modifications and the roles they play in inheritance/disease.

Nov. 19, 2015, 3:30 p.m.

Freshers Fairs introduce young scholars to the opportunities available to them at University. Read about some of the scientific societies that attend Freshers Fairs in the UK.

Nov. 17, 2015, 3:30 p.m.

Addgene participated in the 2015 MassBioEd career exploration day to teach students some of the great careers available in biology.

Nov. 13, 2015, 3:30 p.m.

Inntags are new epitope tags designed to minimally interfere with protein folding. Find out more about Inntags in this blog post.

Nov. 10, 2015, 3:30 p.m.

The International Mentorship Program brings together students and mentors across Europe to develop the skills needed to succeed in a globalized world.

Nov. 6, 2015, 3:30 p.m.

It can be difficult to choose the best CRISPR software tool. The CRISPR Software Matchmaker makes choosing a guide RNA design software easier.

Nov. 3, 2015, 3:30 p.m.

A brief summary of the history of plasmids and their use in cloning and molecular biology.

Oct. 29, 2015, 2:30 p.m.

Fluorescent proteins can mis-fold or form aggregates outside of the cytoplasm leading to errors in localization. Avoid these issues with new mOX-FPs.

Oct. 27, 2015, 3 p.m.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Oct. 22, 2015, 2:30 p.m.

The Future of Research Symposium Boston 2015 aims to develop solutions to problems in academic research. Learn how you can get involved .

Oct. 20, 2015, 2:30 p.m.

Cpf1, a newly discovered Cas9 homolog, is smaller than Cas9, can target different sequences, and may more efficiently generate targeted mutations.

Oct. 14, 2015, 2:30 p.m.

In this blog post, learn how Addgene tracks samples and how you could implement a similar system in your lab.

Oct. 6, 2015, 2:30 p.m.

This 3rd and final post of our Primer on Optogenetics has tips to help you design your own optogenetics behavioral experiment.

Oct. 1, 2015, 2:30 p.m.

Learn about pSiM24, a new binary vector drives gene expression in plants.

Sept. 29, 2015, 2:30 p.m.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Sept. 24, 2015, 2:30 p.m.

Learn about how ClonTracer, a lentiviral library for lineage tracing, was used to test if cancer cells have resistance to drugs before or after treatment.

Sept. 22, 2015, 2:30 p.m.

In this 2nd post of our three post series on optogenetics, learn about the hardware you'll need to perform optogenetics experiments in mice.

Sept. 17, 2015, 2:30 p.m.

Addgene recently awarded $5000 in plasmids to rare disease researchers as part of the BeHeard Award. Learn more about the award here.

Sept. 15, 2015, 2:30 p.m.

Learn about key considerations when conducting optogenetics experiments in mice, including how to pick and delivery an opsin and stereotaxic surgery tips.

Sept. 10, 2015, 2:30 p.m.

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.

Sept. 8, 2015, 2:30 p.m.

Learn how the team building atmosphere at Addgene keeps its employees excited about helping researchers share their science.

Sept. 3, 2015, 2:30 p.m.

A laboratory research management consultant and coach provides step-by-step advice on how to negotiate a budget when stating your lab.

Sept. 1, 2015, 2:30 p.m.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Aug. 27, 2015, 2:30 p.m.

Learn about what it takes to start your own business in the biotechnology field.

Aug. 25, 2015, 2:30 p.m.

This blog posts details what it's like to be a grant writing professional and how to get into the grant writing profession.

Aug. 20, 2015, 2:30 p.m.

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Aug. 18, 2015, 2:30 p.m.

Addgene's Advisory Board helps steer and promote Addgene in its goal of sharing science. We welcome CRISPR expert, John Doench, to the Advisory Board.

Aug. 13, 2015, 2:30 p.m.

This blog post explores how CRISPR/Cas9 can be used to visualize multiple intrachromosomal loci in vivo and map the 4D nucleome.

Aug. 11, 2015, 2:30 p.m.

This blog post explores ways to gain leadership experience at professional organizations like the National Postdoctoral Association.

Aug. 6, 2015, 2:30 p.m.

This blog post explores how commonly used GFP variants can undergo Green-Red photoconversion.

Aug. 4, 2015, 2:30 p.m.

Interview with Professors Eric Mendenhall and Richard Myers about their CRISPR- based system for tagging any endogenous gene of interest.

July 28, 2015, 2:30 p.m.

Makerspaces provide a place for self-directed research. Learn how members of a Makerspace in Victoria Canada model worm locomotion.

July 23, 2015, 2:30 p.m.

Angela Depace's lab recently described how Individual development plans (IDP's) help advance their research. Read on to learn more about IDPs.

July 21, 2015, 2:30 p.m.

Developments in light sheet fluorescence microscopy (LSFM) and tissue clearing enable researchers to visualize tissues of transgenic and non-transgenic organisms in 3D.

July 16, 2015, 2:30 p.m.

It pays for scientists to have a great resumes and CVs. Follow these simple steps to help get you and your resume noticed by future employers.

July 9, 2015, 2:30 p.m.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

July 7, 2015, 3:36 p.m.

Learn how Tyler J. Ford, an Outreach Scientist at Addgene, transitioned from graduate school to working at Addgene.

July 2, 2015, 8:37 p.m.

Overview of the Sleeping Beauty transposase system and how they are efficient tools for genome engineering.pl

June 30, 2015, 2 p.m.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

June 23, 2015, 8:37 p.m.

The pCri plasmids available through Addgene allow researchers to multiplex the protein purification process saving time and frustration.

June 19, 2015, 3:08 p.m.

Science in the News (SITN), a Harvard group dedicated to making science understandable, recently hosted DayCon, a science conference for the general public.

June 17, 2015, 9:04 p.m.