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Showing: 801 - 831 of 831 results

  2. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ... jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting state and its calcium-bound...

  3. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management...

  4. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...transfection into mammalian cells is fairly straightforward and the resultant cells can either express ...

  5. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled ...

  6. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...neuroscience with ever more efficient tools for light mediated neuronal control. The advantages of optogenetics...

  7. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ... an antibody labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region. ...

  8. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...

  12. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...

  17. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...for a lay readership, and working with middle and high school students. One of these organizations was ...

  22. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...requestors use the plasmid in their own labs. We highly encourage depositors to provide plasmid maps whenever...

  23. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...constructs, ADAR2DD(E488Q/T375G) (REPAIRv2) had the highest on-target efficiency and the lowest amount of off-target...

  24. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...vectors all have a genomic capacity that is much higher than other viral vectors (tens of kilobases; compared...

  26. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...bacteria deploy proteosomes to degrade what the cell might consider junk protein. Though there are a number...

  29. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...hosts. Also, self-transmissible plasmids may have high copy numbers and reduced metabolic load (Meyer 2009...

  30. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...externally. Fruit flies are also relatively straightforward to manipulate genetically. Scientists can make...

  31. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...transduce multiple tissues. So how do you choose the right serotype? Keep asking questions!  Table 1: AAV Serotypes...
Showing: 801 - 831 of 831 results