We narrowed to 856 results for: IGH@

...faculty and introducing the kits to clients who might initially be doubtful of online science lab classes...

...productivity by providing world-wide access to curated, high-quality data and reagents Addgene makes it easy ...

...pinpoint exactly when I realized I had made the right decision to choose Addgene for my co-op, but I know...

...Antibody dependent cellular cytotoxicity (ADCC) is a slightly more direct method than opsonization. In ADCC,...

...recommended names and synonyms will all get you to the right entry.) You can look up the cell lines you are currently...

...consider. Antibiotic selection Selection is straightforward in cases where the inserted DNA includes an...

...document may sound like something out of your nightmares, shared ownership of materials produced from ...

...troubleshooting tips to make plasmid usage as simple and straightforward as possible. If you have a question about a...

...an opportune time or go all out with constitutive high level expression? Finally, you can add bells and...

...and center, lending our look some continuity. Our bright color palette was chosen to help convey our personality...

...-enveloped virus (left) and an enveloped virus (right). Created using biorender.com   If you’re new to...

...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields are...

... the same protein, their gene sequence may have slight or large variations depending on how closely related...

...fertilizer, seeds and a growth space with fluorescent lights. Other experiments require only seeds, petri dishes...

...of troubleshooting failed experiments. Pick the right strain While common E. coli strains like DH5-alpha...

...biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions...

...elements Coding sequence features are relatively straightforward to define, but for control elements such as...

...amplify your insert DNA. It is important to use a high fidelity polymerase to minimize mutations. The fidelity...

...Then ask for their honest opinions -- this part might hurt, but if you pitch to enough people, you will...

... and human embryonic stem cells, they found very high recombination percentages (78-79% after two rounds...

...Watson-Crick base pairing, allowing for rational, straightforward design. Since the technique relies on base ...

...knowledge to impart. An undergraduate can mentor a high school student, a graduate student can mentor an...

...business consulting, mathematics, and more. That’s right, we have non-traditional scientists working in our...

...and, depending upon who your challenger was, you might have to drastically alter your strategy. I realized...

...back actual magic (which had been lost for an age) fight in the Napoleonic Wars and against something even...

... jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting state and its calcium-bound...

... Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management...

...transfection into mammalian cells is fairly straightforward and the resultant cells can either express ...

...makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled ...

... an antibody labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region. ...

...neuroscience with ever more efficient tools for light mediated neuronal control. The advantages of optogenetics...

...NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...

...In many cases, CRISPR may be more efficient. You might often start with CRISPR/Cas9, but TALENs do have...

...is important to know that the student presenting right before me had been assigned a structure paper with...

...needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their...

...actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice thing about...

...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...

...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...

...western blot as normal! Stripping your membrane Alright, so you’ve dried your membrane and returned it ...

...education. If you suspect that science communication might be for you, don’t wait. Start communicating now....

...for a lay readership, and working with middle and high school students. One of these organizations was ...

...species one is working with, the protocol will slightly differ. For example, Girardia sp. and Dugesia ...

...your desired genes. Remember Punnett squares from high school biology? They will come in handy here! Crossing...

...supplementation with grant funds, money is always tight. Of course, unlike your traditional biology lab,...

... job appealed to us. However, since the lab was highly focused on protecting intellectual property, it...

...Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “Highly efficient CRISPR mutagenesis by microhomology-mediated...

...requestors use the plasmid in their own labs. We highly encourage depositors to provide plasmid maps whenever...

...some classic protocols on Protocol Online that you might find useful for your research. LabGuru Website: ...

...vectors all have a genomic capacity that is much higher than other viral vectors (tens of kilobases; compared...

...constructs, ADAR2DD(E488Q/T375G) (REPAIRv2) had the highest on-target efficiency and the lowest amount of off-target...