We narrowed to 610 results for: ACE;
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TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....adjustments in the physical organization of your workspace and your workflow can have a huge impact. Test... experiments at odd hours, used more secluded workspaces, or politely asked to not be disturbed when they...
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Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m....legends who not only survive, but thrive, in the face of some of Earth’s harshest environments: corrosive...out rapidly in warm weather. Psychrophilic enzymes face very similar issues when forced to express in mesophilic... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog PostPublishedDec. 12, 2019, 2:22 p.m....information, however, did not improve at the same pace as the quantity and quality of the information. ...down, and zooming out — with an intuitive user interface. The team approached the issue from multiple... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m....endeavor. Due to the COVID-19 pandemic, students will face a reduced supply of internships as a result of the...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m....outside your organization. The mentor in your own workplace is able to: Help you identify specific skills...can tap for email discussions. It is also a great place to practice your mentoring skills. Online mentoring... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m.... on size is ultracentrifugation. Sedimentation equilibrium analytical ultracentrifugation yields an affinity... -
Viral Vectors 101: An Introduction to AAV
TypeBlog PostPublishedMay 30, 2023, 1:15 p.m....in recombinant AAVs (rAAVs) with maximum “trunk” space for genetic cargo. Typically, when researchers speak...dependent switches control gene expression via switches placed in your cell type of interest. Careful combination... -
Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m....communication, be it in your academic lab or your workplace, is an ongoing process. It is never really done...you try any of these approaches in your lab or workplace, let me know. If you have come upon any other ... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....collaboration. The first iteration of these workshops took place at Caltech in Jan 2018 and involved presentations...dedicated website to host all the materials in one place and better serve researchers at all levels of the... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....entails will help keep you motivated when you’re faced with yet another hurdle in your research journey...will always be room to improve. The next time you face a problem, try to be mindful of how you work through... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....Figure 1). This light-induced excitation can take place in a tissue culture dish but can also happen in ... motor cortex (M2). Since M2 is located on the surface of the brain, to deliver light to the channelrhodopsin-expressing... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m....understand so that we could possibly develop pharmaceutical interventions that would inhibit IL-36 release...intensity-based signaling biosensors to monitor intracellular signaling,” says Hébert. Previous research has... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m.... RNA editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting...development and use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m.... et al. (2018). Of course, preprints don’t replace journals and other modes of scientific communication...journal and share it more widely! Not everyone embraces preprints as part of their scientific communication... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....developed to replace helper viruses in AAV production systems. We’re just skimming the surface of the natural... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....from the drop-down and add an explanation in the adjacent Patent/License Explanation field. Filling ...in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of your PI and send the... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m.... Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make....9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More information... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m.... to undergraduate students (1, 2), but educators face significant challenges in designing suitable projects...complementation should only occur when galactose replaces glucose as the carbon source. Student results ... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....SARS-CoV-2 virus depends on its spike protein to bind ACE2, the host cellular receptor. To more easily study...SARS outbreak, Hyeryun Choe’s lab identified that ACE2 is the functional receptor for SARS-CoV. The lab... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m.... specific transcription factor binding sites interspaced with random sequences. Strategy #4 (ProD): ...where lower levels of labeling makes it easier to trace a neuron’s projections. Generating animal models...