Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 847 results for: CAL;

Showing: 321 - 340 of 847 results

  1. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...environment? Does the group expect each other to be physically present at certain times during the day? We asked...history in science. Potential graduate students typically go through a rotation, which reveals their working...job satisfaction (especially for scientists and technical people) is linked to engagement and access to ... "chaotic" and offer less micromanaging, they typically offer more flexibility in choosing direction of...

  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...such as plasmids) into mammalian cells, a process called transfection. Transfection is somewhat comparable... way. Selection methods, described below, are typically employed when creating stable cell lines and are...respectively). Therefore, it is not always mission critical to make sure the plasmid replicates in the cell...   ***The concentration used for selection is typically more (double) than that used for maintenance of...usually achieved by performing a "kill curve" (basically growing cells in various concentrations of the...resistance; however, like gentamicin, neomycin is typically used to suppress bacterial growth. The selection...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...manipulating interneurons using a viral approach Genetically modified organisms like transgenic mice are important...a synthetic red fluorescent protein variant they call mScarlet. Bindels et al used a combination of deliberate... cell physiology and molecular processes via genetically encoded proteins. PhoCl is a new member of this... such as an NLS or NES, enables control over localization. Inserting PhoCl between an enzyme and an inhibitor...experiment, check out our recent guest blog series: A Practical Approach to Choosing the B(right)est Fluorescent...investigated an aspect of telomere length regulation called telomere trimming and have proposed a role for ..., Li and colleagues observed endogenous TZAP localization at telomeres and found that TZAP binds directly...

  4. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...specific subcellular localization peptide. By combining three different localization peptides with four ... for example).  Given that the liver is an immunologically active organ, this advance is likely to afford...off-target mutations. These plasmids contain catalytically inactive dCas9 and microbial SSAPs (dCas9-SSAP...system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient and less risky.   ...plex mixed-subclone system using mStrawberry, localization peptides, and reporters that drive the expression...

  5. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...variables - your approach, your workflow, or your physical setup - to prevent the mistake, just like you ...differently.  Tools to Try  Checklists Found in surgical suites, air fields, and military bases, checklists...light-sensitive reagents” or “re-sterilize now.” Have a physical copy of the protocol when you work and make a ...time to do them carefully and thoroughly. Have a physical copy on hand as you run your experiments. Try ...your plate maps onto your plates, alongside your physical copy. If you’ve got messy handwriting, reach for...accuracy.  Bench setup Small adjustments in the physical organization of your workspace and your workflow...

  6. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...lower-energy photon; no chemical reaction takes place. Figure 2: Simplified chemical reaction catalyzed...American firefly, Photinus pyralis, catalyzes a chemical reaction in its abdomen to produce yellow-green...bioluminescence, is the production of light by a chemical reaction. In contrast, fluorescence occurs when...BioRender.com. Bioluminescent reporters require a chemical substrate (and sometimes cofactors) to be provided...   Luminescence Fluorescence Source of light Chemical reaction High-energy photons Kinetics of photon...

  7. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...This process is called α-complementation. The system described above was put to practical use in the following...well-characterized bacterial lac operon contains a gene called lacZ that encodes for the enzyme β-galactosidase...β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside...deleting a section from the lacZ gene (a mutation called lacZΔM15) creates a non-functional β-galactosidase...Providing DNA encoding this section of amino acids (called the α-peptide) to a lacZΔM15-mutant bacterial cell...

  8. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...proteins yet they still share many of the same technical advantages that come with working with prokaryotes...mutation. Yeast expression plasmids used in the lab typically contain all the necessary components to allow ...are small independent chromosomes and are thus typically found as a single copy. Unlike the ARS vectors...promote replication.  Selection markers for yeast Historically, scientists have utilized auxotrophic selection...plasmid may be expressed at higher than normal physiological levels due to high copy numbers. This creates...the presence of the selection marker at non-physiological levels. Scientists have tried varied approaches...

  9. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...limitation and allows for the flexible design and small scale production of antibodies. Problems with antibodies...instead of via recombinant DNA, they can’t be genetically tagged and instead are conjugated to reporters...labeled tyramide molecule into a highly reactive radical, which is then covalently linked to residues within...however, compatible with most multi-labeling immunohistochemical protocols where either a directly labeled... required for detection RANbody is detected enzymatically with HRP substrate More sensitive than detecting...RANbodies) as versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional...

  10. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...composed of four peptide chains (two identical heavy chains and two identical light chains) each with a constant...antibodies means that it is relatively easy to physically divide up the different regions, both through...joined by a linker peptide, resulting in a product called a single-chain Fragment Variable (scFv). Size: ...samples more efficiently and can also improve localization accuracy when used in super-resolution microscopy...David vs. Goliath: The Structure, Function, and Clinical Prospects of Antibody Fragments. Antibodies 8(...

  11. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ... audience and convey your ideas clearly and strategically is powerful in almost any career. It allows ...my head. What came out, though, was an incredibly calm: “Excuse me, I seem to have lost my place. I need... the open windows in the middle of your talk. Technical difficulties turn your slide deck into a chalk...everyone! Everybody makes mistakes, encounters technical difficulties, and runs into unexpected stumbling..., to a group of peers who are there to provide critical feedback, often with a semi-international audience...your own native language. And, at least in the biomedical field, with very little text on the slides and...

  12. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...your knowledge at local schools. Or work as a guide in local museums, zoos or botanical gardens. You can...unusual setting: Discuss your latest research in a local pub at a Pint of Science event. Or get on stage ...to reach young people? Try networking with your local education community. Get involved in education advocacy...communication organisation like Building with Biology. Local environmental groups, charities and NGOs are often...

  13. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ... have a quiet place to take your video call.  Expect technical difficulties. To try and circumvent this...ago. I just recently started working in the pharmaceuticals industry after earning my Master’s in 2016 ...presentation as an opportunity to demonstrate technical and transferable skills that make you qualified...perfect science talk Be prepared for potential technical difficulties. Have your presentation on a flash...from HR carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably...

  14. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...very useful in the event that you will not be physically present at your poster at all times, so other...readers can navigate your poster and follow the logical progression of your work, and you can accomplish...offers free poster templates, although most are vertical.  Large central box. Another commonly used and...presentation. Portrait. This style displays a poster vertically rather than horizontally. Instead of displaying.... Shapes. In this type of poster, written and graphical information are inserted into larger circles or... or ovals, that are arranged in some logical pattern across the poster. This style has the advantage of...

  15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ... be used for measuring protein expression or localization in cells, or even single-particle tracking of...named according to the number of strands in the canonical beta-barrel structure. Most frequently, the proteins...fluorescence complementation analysis under physiological conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques...fluorescent proteins as tags and biosensors for multiscale imaging. Shcherbakova et al. Nat Commun. 2016...split-FP options. While the BiFC interaction is typically irreversible and requires some time for chromophore...

  16. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...Mother Nature’s toolbox. Natural plasmids, also called environmental plasmids, are all around us! A plasmid...carrying genes for bacterial toxins like colicin typically also carry the necessary resistance genes to help...cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred...which strains of E. coli contain this F plasmid is critical for successful Gateway cloning. In addition, scientists... Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated gene transfer in...Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology, 24(4), 155–208. https...

  17. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...histone-derived peptide) which can localise the construct specifically to the histone protein of interest...becomes. This prevents the cellular machinery from physically interacting with the genes in the compacted region...grandchildren; in this study,epigenetic changes were statistically significantly associated with reduced life-span... Chi-Wang, Cindy Y. Jao, and Alice Y. Ting. "Genetically encoded fluorescent reporters of histone methylation...methylation in living cells." Journal of the American Chemical Society 126.19 (2004): 5982-5983. PubMed PMID:...

  18. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...several ways. Below is a table listing some of critical differences (adapted from Zhou and Melton, 2008...this process. Moreover, it is much easier to genetically modify cells during the reprogramming process...This opens up a wide range of possibilities in clinical situations. In cases where the objective is to...Kushi) Mukherjee is the Editor of Trends in Pharmacological Sciences, a Cell Press reviews journal. She...from Massachusetts General Hospital and Harvard Medical School. Connect with her on LinkedIn @ https://...PMCID: PMC2854424. 23. Burridge, P.W., et al., Chemically defined generation of human cardiomyocytes. Nat...pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012....

  19. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...systems. FPs have been used to track protein localization, cell structure, intracellular trafficking, ...tagged proteins can lead to improper protein localization, protein aggregation, or disruption of normal...Also, it remains challenging to screen for the genetically modified strains that contain your newly tagged...recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR genomic tagging in C....Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR tagging systems can be ...endogenous proteins for studying protein expression, localization, and protein-protein interactions. Additionally...

  20. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...Chavez, researchers at the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 technology...has facilitated the development of a simple and scalable means of making targeted genetic edits. Cas9 is... a gene of interest, they have been shown to dramatically alter levels of expression. Cas9’s ability to...Lance-Byrne is a graduate student at the University of California, Santa Cruz, where her research is focused on...Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease." Nature biotechnology ...Options for CRISPR Genotyping Cas9 Activators: A Practical Guide Additional Resources on Addgene.org Check...
Showing: 321 - 340 of 847 results