Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 571 results for: CHI

Showing: 321 - 340 of 571 results

  1. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...RNA that bind to a cell’s DNA, telling the Cas machinery where to cut.      Fig. 2: Heatmap indicating...when interpreting findings, particularly when researching ancestry groups. And, of course, it’s imperative...

  2. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... Chemistry at Connecticut College who has been teaching and communicating science for over two decades...as pertinent now as it was over 300 years ago. Teaching by example No examination of the state of science...

  3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...fluorophore can emit a limited number of photons before bleaching. The amount of information one can extract from...cell biologist can feel like a college student searching couch cushions for spare change, desperate to ...

  4. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol. 1983;100:293...2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical...

  5. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ... yourself, “I’m not married,” or “I don’t have children, so my life will be under control.” Think again...starve while you are away a few days. Also, your children will enjoy day care and school if you tell them...

  7. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...efficient base rewriting in the genome became achievable in 2016 when two independent groups (Komor et...is either repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or...

  8. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...rate dependence of transfer RNA abundance in Escherichia coli." The EMBO journal 9.13 (1990): 4359-4366...rate of full‐length human proteins expressed in Escherichia coli." Protein Science 19.7 (2010): 1312-1326...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...streptavidin-binding strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin) and Sun ...Spaghetti Monster is a symbol of opposition to the teaching of intelligent design in public schools and was...

  10. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...particular topic. Alternatively, maybe you’re researching a topic for which no relevant or recent review...Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your...

  11. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...Videos Over at the Addgene Youtube Channel   After watching these trends for a few years, I decided to look... brief introduction to the research and, after watching the video to determine if you’d like to learn ...

  12. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...Reddit Science is heavily moderated. I started watching the questions and comments go up early in the ...mechanics of doing the AMA were interesting. I started watching the questions and preparing answers early in the...

  13. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ... a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver and Budak,...plants." Plant Biology 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed...

  14. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...hope that a similar reversal may be possible for children and adults with Rett Syndrome.  Since these discoveries...these approaches, such as delivery of the editing machinery to the brain, minimizing off-target editing, and...

  15. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV...

  16. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Grover M. Hutchins. "Henrietta Lacks, HeLa cells, and cell culture contamination." Archives of pathology...

  17. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...the quantitative increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed to...Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus.   ...

  18. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...protein products, which can prevent the culture from reaching the desired density within a reasonable time-frame...cystosolic protein products, but this is not always achievable. Proteins which contain hydrophobic regions or...

  19. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...biotin, avidin. Avidin was aptly named for the chicken, “Avian”, egg it was purified from. Snell later...Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...

  20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...retrievable, searchable and traceable. However, switching from paper to an electronic lab notebook requires...within your lab? In every lab, there are certain hierarchies and restrictions to data access. If these are...
Showing: 321 - 340 of 571 results