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We narrowed to 520 results for: EMB

Showing: 321 - 340 of 520 results

  1. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...and equal protein transfer from the gel to the membrane. A loading control is critical for data interpretation.... Always probe the loading control on the same membrane that the target protein was measured on.    Flow...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute.... While this seems obvious, it is important to remember that the same is true when designing gRNAs for...

  3. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...enhancers. Although the lab used this vector in chick embryos, it also functions in mammalian expression systems...vivo cis-regulatory interactions in the chicken embryo. BioRxiv: https://doi.org/10.1101/135525. Find...

  4. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...selection marker, then electroporate it into mouse embryonic stem cells. After selecting for clones with the...the mutation and inject the ES cells into a mouse embryo to create chimeric mice. This laborious process...

  5. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...Show suitability for an intended application - Remember, just because your antibody performs as expected...is just as helpful as knowing when it will. And remember, antibodies are like puppies. Just because an ...

  6. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...the background. Dyes that can pass through cell membranes can prove useful for live-cell imaging. Experimental...coupled receptors. Biochimica et Biophysica Acta - Biomembranes, 1838(1), 15–33. https://doi.org/10.1016/j.bbamem...

  8. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. Fourth-generation cytosine...Tsang in August 2020 and by Emily P. Bentley in September 2025. References and Resources References Chen...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...via genetically encoded proteins. PhoCl is a new member of this class that works via a different mechanism...ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. These exciting papers ...

  13. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...tagged with #DeckTheLab throughout the month of December are eligible to win a hot chocolate party that...

  14. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...to Addgene, Aavery!       Original post: In December, we introduced you to Blugene’s new virus friend...

  16. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...! Executive Director Chonnettia Jones and Board Member Joanna Brownstein were also on the welcoming committee...

  19. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...across 55 countries.  Our first request was on September 8, 2004 (for pcDNA-p160MBP, if you're curious....

  20. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...gene-editing, the foundations of synthetic biology, DNA assembly, and various applications. I think these papers...
Showing: 321 - 340 of 520 results