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We narrowed to 680 results for: Eng;

Showing: 321 - 340 of 680 results

  1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...blog post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient...prevented. The loxP and FRT target sites have been engineered to avoid this re-inversion issue. It became possible...of the asymmetric target site core but its 8 bp length that is critical for Cre and FLP function. Because...

  2. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...pyogenes (SpCas9), is truly an exceptional genome engineering tool. It is easy to use, functional in most species...bacteria) Cas13a targeting with a single crRNA The Feng Zhang lab used Cas13a from Leptotrichia shahii (...cleavage with spacers from 20-28 nucleotides in length and does not require a specific PFS, making it ...

  3. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...of 340 institutions with postdocs in science, engineering and health from the NSF’s 2014 Graduate Student...what they are doing, however, may face greater challenges. Current salary information that we have been...current postdoctoral appointments may shorten in length; that postdoc salaries will have to double at some...

  4. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...and significance, these receptors have proved challenging to study. Cells often express multiple integrins...affinity. This extended-open state is key to strengthening ligand binding, mediating cell adhesion and ...adhesion. The results surprised the investigators, challenging the long-held belief that αVβ3 would be the most...

  5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... This test can be performed in two steps for challenging samples as in Two-Step-SHERLOCK or in a fast,...Highlights: To detect non-PAM regions, PAM can be engineered into the primers. This method does not depend.../doi.org/10.1101/2020.04.07.028167 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao...

  7. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...technology has made it easier than ever both to engineer specific DNA edits and to perform functional screens...really due to the intended edit rather than some passenger variant that came along with the single cell clone...development of SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers additional PAM options...

  8. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...12259: pMD2.G (depicted on the left) is ~5.8 kb in length. The plasmid can exist on as a dimer, depicted ...tandem, resulting in a plasmid that is twice the length of the monomer (~11.6 kb). Image generated by SnapGene...and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43. doi: 10.1002/bit.22347...

  9. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...introduces a ssDNA nick, a dsDNA break, or is engineered not to cleave DNA and instead activates/represses...acknowledged as a potential limitation in a study. Strength in numbers If you assess your off-target frequency...Off-target effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389...

  10. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...Preps at Addgene 2) Comparison of genetically engineered receptors (i.e. chemogenetics vs. optogenetics...transduction efficiency, and safety profiles should strengthen its appeal for the scientific and medical communities...techniques: exploiting the offspring of genetic engineering." Journal of neuroscience methods 235 (2014):...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...then flow cytometry can help you overcome these challenges and make these types of experiments easier and...fluorescent signal that is captured by detectors. The strength of the emitted photons varies from cell to cell...fluorophores Fluorescent antibodies get around the challenges of fluorescent protein fusions by binding to ...

  12. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

  13. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...enabling a broad spectrum of donor fluorescence wavelengths for FRET experiments. However, remember that,...amino acid at a stop codon (often amber i.e. UAG) engineered into a desired site. During translation, the ...molecules to each Cy dye, as well as by extending the length of the histidine tag from 6 to 10 residues. While...

  15. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...the second day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. ...CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth day of CRISPR, Addgene...

  16. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...temperature reduces background noise but increases the length of your assay. The most common options are 4 °C...background noise as well, and multiplexing is challenging and limited. Direct westerns typically rely on...waste. However, they also add time to an already lengthy protocol, require the use of hazardous chemicals...

  17. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    Published
    Sept. 5, 2018, 12:01 p.m.
    ...and Jillian Parker! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...

  18. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...-defined contours. (Many of them are software engineers, surprisingly, who have deep technical knowledge...editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also writes Codon, a blog...

  19. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ...twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  20. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...Bacterial Expression Systems. Check out our Genome Engineering Overview and our CRISPR guide to find out how...how CRISPR is being used for bacterial genome engineering. To make it easier for you to find plasmids ...
Showing: 321 - 340 of 680 results