We narrowed to 388 results for: HAL;
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TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....and can therefore be cultured in media containing half the normally recommended antibiotic concentration...
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PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....cell lines can be created in about a month and a half, without the need for complicated cloning of homology... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....fluorescent proteins. Visualizing fluorescence can be challenging in plants because of the auto-fluorescence of... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....allergy testing lab, and the grad student down the hall from you have in common? All of them rely on standard... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m.... method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation’... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m.... to amplify away from the insertion site. Encode half the new sequence at the 5’ end of each primer, with... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m.... completing her PhD and medical training in Ophthalmology. Gene therapy was in its infancy, but the eye... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....graphics for visuals. However, it’s much more challenging to design a hands-on interactive activity using... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m.... like other viral vectors, though significant challenges remain (Wold & Toth, 2013). While the pre-existing... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was the broad... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....presents itself even outside of work, consider challenging yourself, take the opportunity, and discover ... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....success” of the journal club. This can be a challengingly vague metric but there are a few things you ... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m.... include: Self-labeling tags (e.g., SNAP-tag, HaloTag, TMP-tag) – These widely used systems consist of... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....functional luciferase only when fusions of the two halves of the luciferase protein interact and enable intein... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....Becoming a grant professional is rewarding and challenging! Jo Miller, GPC, CSMS is the founder and principal... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....and when they come into close proximity, the two halves of EGFP undergo folding, maturation, and fluorescence... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m.... up the pace Our QC team constantly faces the challenge of keeping up with the large amount of QC analysis... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....persisted despite our best efforts. With a little over half of our orders coming from researchers outside the...