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We narrowed to 821 results for: LAS

Showing: 321 - 340 of 821 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...problem of sharing plasmids, providing researchers with a one-stop shop for plasmids and vectors, and making...other tissues. At the Nuclear Receptor Signaling Atlas (NURSA), one of our primary goals is to promote ...progress that has been made in the NR field over the last thirty years or so would not have been possible ...development of a vast number expression and reporter plasmids and the free exchange of these vectors between...increased the burden associated with identifying plasmids in the research literature and acquiring them ... feature direct links to listings of available plasmids for our curated NRs and coregulators. We look ...edits the website of the Nuclear Receptor Signaling Atlas (NURSA) at www.nursa.org. Bert O’Malley, M.D.,...

  2. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...lentiCas9-blast. A549 cells were transduced (MOI = 37) and selected with 1 µg/mL blasticidin for 9 days...18007609. Addgene Materials Used 1. lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described...BRDN0000561167) (Plasmid #80034) and BRAF gRNAs (BRDN0001145345 and BRDN0001146266) (Plasmid #76479 and #76480...transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some cells were then used ...expression in A549 cells transduced with lentiCas9-blast. Anti-Cas9 Western blotting was performed on whole-cell...then expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed... PubMed Central PMCID: PMC4486245.2. pXPR_011 (Plasmid #59702) was a gift from John Doench & David Root...

  3. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...analyzing the plasmid and viral DNA sequences and expression profile at multiple steps from plasmid amplification...to flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While this strategy greatly...recombination that are typically employed during viral plasmid amplification (Fischer, KB. 2019). This means that...of this finding, we now include guidance on the plasmid page of DIO/FLEx AAV vectors where this occurs ... mechanism of spontaneous reversion during AAV plasmid amplification. On the left homologous pairs of ...specific” promoter, such as the truncated tyrosine hydroxylase (TH) promoter for catecholamine neurons or glial.../FLEx vectors for off-target expression.  Some classes of neurons transiently express TH during development...

  4. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...at the cells on the plate. For example, if your plasmid contains a gene encoding a blue chromoprotein, ...colonies to determine which cells have taken up the plasmid. Of course, other follow up methods like sequencing... chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed, asPink, spisPink, scOrange,...chromoproteins. In some cases, cells containing high-copy plasmid-borne chromoproteins lost color overnight in liquid...problem. There is no single “ideal” chromoprotein plasmid that combines the most desirable features: intense...Chromoproteins for allelic exchange Figure 3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus...sequential homologous recombination events: The plasmid carrying the desired genetic modification integrates...

  5. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...Allen Institute for Cell Science Plasmid Collection Check Out Plasmids for FRET Check Out the Fluorescent..., most secreted proteins are produced in the endoplasmid reticulum, modified in the golgi and then transported... on the gene names in the figure below to find plasmids containing that particular gene. ... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers Institute...Prescott (Monash University). You can find more plasmids for labeling your subcellular structure of interest...interest in the Allen Institute for Cell Science Plasmid Collection. You can also use the library of full-length...Localization Poster here! References 1. Allen Institute Plasmid Pages 2. Cooper GM. 2000. The Cell: A Molecular...

  6. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ....  This is in contrast to confocal laser microscopy where the laser and the reflected light travel through...Voie, David Burns and Francis Spelman focused a laser beam into a thin sheet to illuminate a fluorescent...than the modern cameras used in LSFM. In LSFM the laser light sheet, typically 2-6  microns, illuminates...-bleaching or photo-damage than in conventional laser scanning microscopy . Since image acquisition is...Ultramicroscope.  In addition, adjusting the thickness of the laser sheet and using multi-view imaging can provide enhanced...compared to transgenic animals (17).  These viral plasmids are available through Addgene.  The combination...University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an...

  7. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids 101: Green Fluorescent Protein Resources...Fluorescent Protein Collection Find Plasmid Kits at Addgene Find Plasmid Cloning Protocols References 1....combination of different genetic sequences into plasmid DNA. In high throughput cloning techniques, although...MXS chaining MXS-chaining was designed to create plasmids for fluorescence imaging or flow cytometry applications...modules (Figure 2) are contained in individual plasmids and are each flanked by the same multiple cloning...throughput cloning is to facilitate the construction of plasmids with many components. The creators of the MXS-... plasmid cloning...

  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ... His solution? Bringing his research into the classroom. I had the opportunity to talk with Provost recently...interest. The result was a positive experience for the class but one that left him overwhelmed. Therefore, he...measured activity, MDH is particularly amenable to in-class labs. Provost ultimately settled on a two-semester...critical thinking skills (Knutson et al., 2010a,b). Classroom-based research projects have also been proposed...professional success (many of my students include their in-class research experience on their resumes). CUREs are... sounds like an exciting opportunity for your classroom, I highly recommend you try implementing one. ...on planarian flatworms both in and out of the classroom.   References and Resources References Bangera...

  9. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Using arabidopsis in the classroom Resources on Addgene.org Find plant plasmids Visit our plant resource...this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium tumeficans - a...available (Weigel and Glazebrook, 2002). Find plant plasmid resources at Addgene  Choosing an expression system...options, but here are a couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway...prefer Golden Gate assembly, there are a series of plasmids from the Dinneny lab that additionally allow for... plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are both great options for this, depending...culture you are growing definitely contains your plasmid. Grow lots of Agrobacterium: The more agrobacterium...

  10. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...enChIP Plasmids? All Hodaka Fujii lab plasmids Sign up to receive email alerts when new plasmids from ... shopping for a wide range of plasmids. The merit of depositing plasmids to Addgene has been spectacular...this technology and its advantages? Fujii: In the last several years, my lab has been working on development... researchers all over the world have requested plasmids for enChIP through Addgene. Retroviral Expression...user-friendly and precise. We will deposit the plasmids of these systems to Addgene in the near future...Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's Advisory...

  11. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs...countries enriched our repository with 15,020 new plasmids — a testament to the scientific community's commitment...next-generation sequencing, setting new standards for plasmid quality control. Through our partnership with the... viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged on demand...toward giving you more choice in how you receive plasmids — as DNA or bacterial stabs — ‌and reinventing...sequences and characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists...developers to unlock the potential of our extensive plasmid database — because we believe that innovation flourishes...

  12. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ... about the last 10 years @Addgene Check out our impressive stats from 10 Years of Plasmid Sharing Or ... into the successful, internationally renowned plasmid repository that it is today. Although I wish I ... founders, Melina Fan, Ken Fan, and Benjie Chen. Last week, the Addgene 10-year Anniversary Gala celebrated...music playing all night was hand-picked (like our plasmid samples!) by Addgenies. If you were there, you ...video highlighting the changes at Addgene over the last 10 years as well as the many people that make up...Addgene uses E. coli bacteria to generate copies of plasmids. The community of scientists, part of the Addgene... complicated process of requesting and sharing plasmids has become simplified through Addgene. Looking...

  13. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ....org Check out Our Hot Plasmids Articles to See Compiled Info on New Plasmids Review the CRISPR Field...beginning. I also recommend using the citation style of (Last name, Year) in the document while writing, because...style to whatever the journal requires. Using the (Last name, Year) format also has the benefit of exposing..., we summarized how frequently each member of a class of proteins was mutated, as reported in various ...We made a table that listed each protein in the class, then for each protein we listed all the studies... we made a schematic of all the proteins in the class that showed the relative sizes and the conserved...similarities and differences among proteins in the class. Offer your perspective It doesn’t have to be long...

  14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm? Or more troubling...other than the cytoplasm. Generally, FPs evolved in or were engineered for use in cytoplasmic environments...mitochondria and chloroplasts, the extracellular milieu, and gram negative bacteria periplasm. Efforts to ...FPs) localized to compartments other than the cytoplasm. That is, unfortunately, some investigators realize...quality control of secretory proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik...mediate mitotic inheritance independently of the endoplasmic reticulum." The Journal of cell biology 154.2...

  15. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...that they called a Glycosylase Base Editor, or GBE, for its reliance on the glycosylase UNG to crease abasic...Converts C → G; also called a glycosylase base editor (GBE). GBE Glycosylase Base Editor A base editor ...Uracil DNA N-Glycosylase Removes uracil from DNA, creating an abasic site. UGI Uracil Glycosylase Inhibitor...at its original job. They removed the uracil glycosylase inhibitor (UGI) components, which inhibit BER...instead introduced its opposite, a uracil DNA N-glycosylase (UNG) from E. coli. The resulting construct, ..., Rosser, S. J., Bi, C., & Zhang, X. (2021). Glycosylase base editors enable C-to-A and C-to-G base changes...Additional resources on Addgene.org Browse CRISPR plasmids for base editing Get a broad overview with our...

  16. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    Published
    April 12, 2018, 1:02 p.m.
    ...More than a model System - Arabidopsis in the classroom With funding from the American Society of Plant... the kits, known collectively as Greening the Classroom, were developed and tested by ABRC staff. These...an ordering account. Using Arabidopsis in the classroom has many benefits. Engaging students in active...science curriculum. Growing Arabidopsis in the classroom does not require a huge investment in resources.... Another benefit of using Arabidopsis in the classroom is that staff from the ABRC are here to support... people in science learning. Whether visiting classrooms, hosting field trips, conducting teacher workshops...resource or you want to introduce Arabidopsis to your class for the first time – we want to hear from you. Reach...

  17. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...Biotechnology which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen....with other fluorescent proteins. Find mNeonGreen plasmids here! mTFP1: a monomeric teal fluorescent protein...492 nm, respectively), they gave this a teal classification rather than cyan and named the protein mTFP1...physiologically relevant changes in pH. Find mTFP1 plasmids here! mWasabi: a bright green fluorescent protein...excite at 400 nm while EGFP does. Find mWasabi plasmids here! One of the most notable things about these...are best suited for certain studies. If you have plasmids containing mNeonGreen, mTFP1, or mWasabi that ...with the scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "...

  18. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts  Suggest Plasmid Tools for the ...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...to changing pH, and they can rupture E. coli spheroplasts to release encapsulated proteins. Video by ACS...these fluorescent R bodies can rupture E. coli spheroplasts (cells which have had their cell walls removed...produced R-bodies that could extend at different pHs. Plasmids expressing expressing both wild type and mutant...acssynbio.5b00237. PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni, ...

  19. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...cite the original paper but link to plasmids from the Promega Plasmid Collection that express similar constructs...Addgene Luciferase 101 Luciferase Plasmid Collection Promega Plasmid Collection ... might wonder what sets HiBiT apart. While the classic Green Fluorescent Protein (GFP) and other fluorescent...incorporate HiBiT with a wide range of workflows, from classic overexpression to CRISPR knock-in, letting you ...some proteins do not have such epitopes. These classical 'undruggable' targets are often intrinsically ...degradation in real time Proteins involved in endoplasmic reticulum quality control navigate a large and...cell and protein analysis products. You can find plasmids deposited by Promega scientists here.  References...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source...useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase itself, please ...genome fully intact, while, with a standard DNA plasmid transfection, a double-stranded break may occur...by utilizing a piggyBac™ transposon in a donor plasmid carrying the DNA edit of interest, along with a...piggyBac™ transposase enzyme [4]. By using a donor plasmid containing a negative selection marker such as ...kill off any cells that still contain the donor plasmid after excision by PBx, thereby leaving behind only...cytotoxicity [5]. Second, instead of using a donor-plasmid, you could use a single-stranded oligodeoxynucleotide...
Showing: 321 - 340 of 821 results