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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 662 results for: MAL

Showing: 321 - 340 of 662 results

  1. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...combination of the three (Servellita et al., 2023). Normally when children have acute viral hepatitis it is...commonly derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family,... adenoviral vectors in Addgene’s repository is smaller than for other vector types, we hope that this ...

  2. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...sterile flat toothpick or pipette tip and swirl in a small amount of sterile water.  Pick 3-10 colonies in ...to start liquid cultures. Lastly, you can start small overnight liquid cultures with the clones you pick...boiling the sample before use or by directly adding a small volume of the sample to the PCR reaction. The bacteria...

  3. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...the 3rd-generation packaging systems offer the maximal biosafety available for the technology. The minor... divided as follows: Transfer vectors contain minimal cis-acting HIV components: LTRs, PPT, RRE, and ...vectors are SIN. Packaging plasmid(s) contain the minimal number of HIV genes required for virus production...

  4. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... Pechmann and Frydman found that tracts of non-optimal codons are associated with specific secondary structures...coli strains express a variety of tRNAs that are normally found at low abundance in E. coli. The advantage... Frydman. "Evolutionary conservation of codon optimality reveals hidden signatures of cotranslational ...

  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...studies demonstrated successful base editing in mammals, but there is a lack of base editors for plant ...PAM). ABE7.10 can edit target 'A' if it lies in a small genomic window: at protospacer position 4-8 counting...Agrobacterium-mediated transformation. We also constructed smaller vectors suitable for transient assays using protoplast...

  6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...COVID-19 (Joung, Ladha et al., 2020). Cas13a in mammalian cells The Zhang lab made the exciting discovery...discovery that some Cas13a orthologs can function in mammalian and plant cells (Abudayyeh et al., 2017). They...superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage with...

  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...lentiviral expression of the human KRAS gene in mammalian cells. STEP 1: Generate an Entry Clone There are...expression level, and experimental purpose. For mammalian lentiviral expression, we could use a vector like...and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning vectors...

  8. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...adapted it for a host of different proteins and small molecules from a variety of sample types (Engvall... competitive or inhibition ELISA when measuring small molecules that cannot bind efficiently to two antibodies... develop assay Competitive Appropriate for small moleculesReduced chance of cross-reactivity Less...

  9. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...impacting prime editing and found the protein La (a small RNA-binding exonuclease protection factor) promotes...2024) Improving prime editing with an endogenous small RNA-binding protein. Nature. 2024 Apr 3. doi: https...trans-splicing. By splitting a large gene into smaller fragments, delivering them by individual AAVs, ...

  10. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...where one plasmid outcompetes the other, usually a smaller, higher copy plasmid can more effectively titrate...Environmental Plasmids blog). Scientists have designed small, high copy incompatible plasmids that lead to asymmetrical...2012) Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural...

  11. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...surprised at how this powerful tool is in such a small package. But don’t let the simple presentation deceive...freeze-thaw cycles needed when using the virus. A small aliquot is used to determine functional titer with...aliquot from an amplification done by Addgene.) This small aliquot is not enough to transfect producer cells...

  12. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...blind spots. Blind spots often result in less than optimal user experience and the overall soundness of the...development. Scrum teams plan and break up work into small, well defined chunks, called stories, and work on... to the story being scrapped or broken up into smaller stories. Since this occurs before a single line...

  13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...optimum of 30 °C and is therefore inefficient in mammalian cells (usually grown at 37 °C). Smart molecular...populations) within neuronal networks in living animals. To accomplish such cell specific manipulations.... “Conditional Site-Specific Recombination in Mammalian Cells Using a Ligand-Dependent Chimeric Cre Recombinase...

  14. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...for permanent reference by the Winnower. The only small downside to the AMA from my end as an organizer/...of great stuff there anyway), but this is just a small quibble. If you are not too worried about selling...resources to the basic questions and to provide a small bit of support (you are not alone!) for those going...

  15. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...reagents and process. It is important to note that optimal experimental conditions, including how much plasmid...backbone as Plasmid A—that does not target any mammalian gene. This non-targeting control is critical to...a gene, you should design a control that shows maximal activation. Likewise if you are trying to repress...

  16. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...pool down to plasmids expressing variants with optimal function. High-throughput DNA sequencing is then...ubiquitination activity assays, toxin activity, small molecule binding, growth rate, and many more. See..., Gibson assembly is better suited for creating small, focused scanning libraries. PFunkel: Of prior...

  17. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ... refer to antibodies and 'staining' to refer to small molecule dyes. Indirect labeling While antibodies...of those CD8+ T cells, require the cells to be minimally altered, so you would not be able to use antibodies... Lastly, stains, based not on antibodies but on small molecule dyes, are useful, most notably when it ...

  18. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...Approach: Viral RNA is extracted and subject to isothermal amplification by primers that target the E and...RNA is extracted from a sample and subject to isothermal recombinase polymerase amplification (RPA) using...contain reverse transcriptase to produce cDNA. Isothermal amplification is done at one constant temperature...

  19. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...Good fixation maintains sample morphology with minimal impact to the target epitope. There are multiple...Sample Preparation This step can involve multiple smaller steps that all help to ensure that antibodies can...Sample Preparation, this step can involve multiple smaller steps, but the aim is to preserve sample integrity...

  20. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...The new SuperPLDs work with high efficiency in mammalian cells and in vitro, opening up a wide realm of...directed-evolution methods, the activity-based selection in mammalian cells used here could be an attractive strategy...Activity-based directed evolution of a membrane editor in mammalian cells. Nature Chemistry, 15(7), 7. https://doi.org...
Showing: 321 - 340 of 662 results