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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: Ncl

Showing: 321 - 340 of 628 results

  1. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...are needed to optimize yield. You might want to include an extra wash step or use a different strain. For.... Look out for carbohydrates Certain strains, including HB101 and derivatives, release large amounts of...

  3. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...terrific getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs...regions” filter unchecked so that these regions are included in the search to maximize the alignment length...

  4. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...mammalian production system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese ...specifically. Possible applications for validation include western blot, immunohistochemistry, and immunocytochemistry...

  5. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...– it makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and...which have fragments of the AdV genome in them, including the E1 region). The space in the viral particle...

  6. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Biology (gDNA → mRNA → protein): their roadmap includes RNA → DNA (Cooper & Temin, 1974), as one of its...will simply express from whatever promoters were included in the vector (or are nearby in the host genome...

  7. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...the primer sequences. Multiple plasmids can be included in a single PCR, for example for subcloning genes...vector. The new codon and homologous region can be included at the 5’ end of either primer. Subcloning - To...

  8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...and its RNA directed binding site. These methods include the fusion of an activation domain such as VP16... or tracrRNA sequences can also be modified to include RNA domains that recruit additional transcription...

  9. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have been reported...al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated purification of a specific genomic...

  10. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... to a new plate containing the mastermix which includes primers and a ddPCR supermix. Note that the supermix...in the detection of microbes. Some applications include measuring viable probiotics and detecting circulating...

  11. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ... have been developed based off of this concept include: Self-labeling tags (e.g., SNAP-tag, HaloTag, ...innovative labeling strategies, several groups—including mine—have been revisiting the old chemistry of...

  12. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...accompanying cartoon, they have a multitude of uses including (but not limited to) purification, detection, ...combinations, though frequently those combinations still include at least one element from the original TAP tag....

  13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ... CRISPR complex much easier in various cells, including primary mouse cells. We believe that this system...sight in that Addgene takes care of everything including MTA and shipping. Addgene realizes the One-Stop...

  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...target the ubiquitin E3 ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein...mScarlet-I3 that were codon-optimized for C. elegans and included artificial introns to improve expression. When...

  15. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...show your enthusiasm for getting the position and include a brief summary of why you are a good applicant...taught for five years at [name of the university]” Include keywords from the job description to help the hiring...

  16. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...right: colony whiteness on inducer plates, white inclusions in cells with GVs via phase contrast microscopy...a time-intensive endeavor. That is why we also included an analogous set of transient constitutively-expressed...

  17. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...method can be used for an array of applications including clinical diagnostics (e.g. pathogen or virus detection...Cas12a is initiated. Surrounding trans-ssDNA, including the ssDNA-FQ reporter are subsequently degraded...

  18. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ... works on our site. For Addgenies, development includes everything from tracking plasmids from the moment...then go back to this area later and tidy it up. Conclusion While none of us are life science majors, we ...

  19. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...to the total number of viral capsids (which can include empty capsids that are devoid of a viral genome... et al., 2010). In addition to the AAV RSM, we include a second AAV reference sample of known titer, which...

  20. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ... in the linings of certain areas of the body, including the lung, the heart, and the abdomen. Long story...primary conventional treatments for mesothelioma include surgery to remove cancerous masses (tumor resection...
Showing: 321 - 340 of 628 results